Monitoring of phosphorus bioavailability in water by an immobilized luminescent cyanobacterial reporter strain.

Massive growth of cyanobacteria, known as "algal blooms", has become a major concern for water monitoring. It has been observed that environmental factors like temperature, light, and certain patterns of availability of nutrients such as P, N, Fe influence cyanobacterial proliferation and toxin production. In order to monitor nutrients in aquatic ecosystems, an assay for monitoring phosphorus bioavailability to cyanobacteria was developed. The test consists of an immobilized luminescent reporter strain of Synechococcus PCC 7942, designated APL. The reporter strain harbours the gene coding the reporter protein luciferase from Vibrio harveyi under control of the inducible alkaline phosphatase promoter from Synechococcus PCC 7942, and can be induced under phosphorus limitation. The resultant CyanoSensor detects PO(3-)(4)-P in a concentration range of 0.3-8 microM after a sample incubation time of 8 h under continuous illumination (50 microE m(-2) s(-1)). The sensor also responded to a variety of organic phosphorus sources and was storable for 3 weeks at 4 degrees C. It could be demonstrated that the CyanoSensor for bioavailability monitoring is an improvement to conventional phosphorus detection methods.

[1]  R. Wetzel,et al.  Uptake of dissolved inorganic and organic bphosphorus compounds by phytoplankton and bacterioplankton , 1992 .

[2]  F. Wagner,et al.  Concomitant Changes in Phosphate Uptake and Photophosphorylation in the Blue-Green Alga Anacystis nidulans during Adaptation to Phosphate Deficiency , 1992 .

[3]  J. Patočka,et al.  The toxins of Cyanobacteria. , 2001, Acta medica.

[4]  T. Mizuno,et al.  The sphR product, a two-component system response regulator protein, regulates phosphate assimilation in Synechococcus sp. strain PCC 7942 by binding to two sites upstream from the phoA promoter , 1994, Journal of bacteriology.

[5]  D. Bhaya,et al.  Isolation, transcription, and inactivation of the gene for an atypical alkaline phosphatase of Synechococcus sp. strain PCC 7942 , 1991, Journal of bacteriology.

[6]  R. Armstrong.  An optimization‐based model of iron—light—ammonium colimitation of nitrate uptake and phytoplankton growth , 1999 .

[7]  J. Keasling,et al.  Modulation of the phosphate‐starvation response in Escherichia coli by genetic manipulation of the polyphosphate pathways , 2000, Biotechnology and Bioengineering.

[8]  L. Sicko-Goad,et al.  PHOSPHATE METABOLISM IN BLUE‐GREEN ALGAE. II. CHANGES IN PHOSPHATE DISTRIBUTION DURING STARVATION AND THE “POLYPHOSPHATE OVERPLUS” PHENOMENON IN PLECTONEMA BORYANUM , 1976 .

[9]  A. C. Redfield.  The biological control of chemical factors in the environment. , 1960, Science progress.

[10]  L. Sicko-Goad,et al.  Phosphate metabolism in blue-green algae. III. The effect of fixation and post-staining on the morphology of polyphosphate bodies in Plectonema boryanum. , 1977, Cytologia.

[11]  Rolf D. Schmid,et al.  Detection of 4-chlorobenzoate using immobilized recombinant Escherichia coli reporter strains , 2000 .

[12]  D. Bryant.  The Molecular Biology of Cyanobacteria , 1994, Advances in Photosynthesis.

[13]  A. Grossman,et al.  Chlorosis induced by nutrient deprivation in Synechococcus sp. strain PCC 7942: not all bleaching is the same , 1992, Journal of bacteriology.

[14]  J. Keasling,et al.  Manipulation of independent synthesis and degradation of polyphosphate in Escherichia coli for investigation of phosphate secretion from the cell , 1997, Applied and environmental microbiology.

[15]  G. Gil,et al.  A biosensor for the detection of gas toxicity using a recombinant bioluminescent bacterium. , 2000, Biosensors & bioelectronics.

[16]  J. Gibson,et al.  Regulation of Phosphate Accumulation in the Unicellular Cyanobacterium Synechococcus , 1979, Journal of bacteriology.

[17]  J. Batterton,et al.  Phosphorus deficiency and phosphate uptake in the blue-green alga Anacystis nidulans. , 1968, Canadian Journal of Microbiology (print).

[18]  M Aizawa,et al.  Fiber-optic-based biomonitoring of benzene derivatives by recombinant E. coli bearing luciferase gene-fused TOL-plasmid immobilized on the fiber-optic end. , 1997, Analytical chemistry.

[19]  G. Jackson,et al.  Importance of dissolved organic nitrogen and phosphorus to biological nutrient cycling , 1985 .

[20]  W. Doolittle,et al.  Control of gene expression in blue-green algae , 1975, Nature.

[21]  B. Masepohl,et al.  The cyanobacterium Synechococcus sp. strain PCC 7942 contains a second alkaline phosphatase encoded by phoV. , 1995, Microbiology.

[22]  J. Houmard.  How Do Cyanobacteria Perceive and Adjust to Their Environment , 1995 .

[23]  W. Simonis,et al.  Photophosphorylation In Vivo , 1973 .

[24]  G. Sayler,et al.  Optical biosensor for environmental on-line monitoring of naphthalene and salicylate bioavailability with an immobilized bioluminescent catabolic reporter bacterium , 1994, Applied and environmental microbiology.

[25]  P. Dillon,et al.  A Simple Method for Predicting the Capacity of a Lake for Development Based on Lake Trophic Status , 1975 .

[26]  T. Mizuno,et al.  Sensor and regulator proteins from the cyanobacterium Synechococcus species PCC7942 that belong to the bacterial signal‐transduction protein families: implication in the adaptive response to phosphate limitation , 1993, Molecular microbiology.

[27]  J. Keasling,et al.  A Dynamic Model of theEscherichia coliPhosphate-Starvation Response , 1998 .

[28]  H. Paerl.  A comparison of cyanobacterial bloom dynamics in freshwater, estuarine and marine environments , 1996 .

[29]  J. Keasling,et al.  Effect of Polyphosphate Metabolism on the Escherichia coli Phosphate‐Starvation Response , 1999, Biotechnology progress.

[30]  Jamie Bartram,et al.  Toxic Cyanobacteria in Water: a Guide to Their Public Health Consequences, Monitoring and Management Chapter 2. Cyanobacteria in the Environment 2.1 Nature and Diversity 2.1.1 Systematics , 2022 .

[31]  T. Mizuno,et al.  A novel gene whose expression is regulated by the response‐regulator, SphR, in response to phosphate limitation in Synechococcus species PCC7942 , 1994, Molecular microbiology.

[32]  A. Post,et al.  PHOSPHORUS BIOAVAILABILITY MONITORING BY A BIOLUMINESCENT CYANOBACTERIAL SENSOR STRAIN 1 , 2002 .

[33]  R. Vollenweider,et al.  Scientific fundamentals of the eutrophication of lakes and flowing waters , 1968 .

[34]  D. Chamovitz,et al.  Carotenoids in Cyanobacteria , 1994 .

[35]  H. Utkilen,et al.  Iron-stimulated toxin production in Microcystis aeruginosa , 1995, Applied and environmental microbiology.

[36]  R D Schmid,et al.  Reporter gene bioassays in environmental analysis , 2000, Fresenius' journal of analytical chemistry.

[37]  H. Utkilen,et al.  Toxin Production by Microcystis aeruginosa as a Function of Light in Continuous Cultures and Its Ecological Significance , 1992, Applied and environmental microbiology.

[38]  How do cells express nutrient limitation at the molecular level , 1995 .

[39]  R. Rippka.  Isolation and purification of cyanobacteria. , 1988, Methods in enzymology.