论文信息 - Scanning slit aperture confocal microscopy for three‐dimensional imaging

Scanning slit aperture confocal microscopy for three‐dimensional imaging

A scanning confocal microscope using stationary slit apertures of variable width is described. Scanning is achieved by employing an oscillating mirror galvanometer capable of scanning images at 160 frames/s. The microscope depth-of-focus is characterized for several slit widths and shown to approach diffraction-limited performance as width decreases. Reduction of flare in images taken with narrow slit widths is demonstrated. In addition, the optical sectioning capability of the system is demonstrated using polymer casts of the vasculature of rat kidney.

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