Inhibition of Receptor Internalization by Monodansylcadaverine Selectively Blocks p55 Tumor Necrosis Factor Receptor Death Domain Signaling*

The 55-kDa receptor for tumor necrosis factor (TR55) triggers multiple signaling cascades initiated by adapter proteins like TRADD and FAN. By use of the primary amine monodansylcadaverine (MDC), we addressed the functional role of tumor necrosis factor (TNF) receptor internalization for intracellular signal distribution. We show that MDC does not prevent the interaction of the p55 TNF receptor (TR55) with FAN and TRADD. Furthermore, the activation of plasmamembrane-associated neutral sphingomyelinase activation as well as the stimulation of proline-directed protein kinases were not affected in MDC-treated cells. In contrast, activation of signaling enzymes that are linked to the “death domain” of TR55, like acid sphingomyelinase and c-Jun-N-terminal protein kinase as well as TNF signaling of apoptosis in U937 and L929 cells, are blocked in the presence of MDC. The results of our study suggest a role of TR55 internalization for the activation of select TR55 death domain signaling pathways including those leading to apoptosis.

[1]  D. Adam,et al.  Induction of stress-activated protein kinases/c-Jun N-terminal kinases by the p55 tumour necrosis factor receptor does not require sphingomyelinases. , 1998, The Biochemical journal.

[2]  M. Krönke,et al.  TNF Receptor Death Domain-associated Proteins TRADD and FADD Signal Activation of Acid Sphingomyelinase* , 1998, The Journal of Biological Chemistry.

[3]  G. Condorelli,et al.  Insulin-like Growth Factor-I Receptor Internalization Regulates Signaling via the Shc/Mitogen-activated Protein Kinase Pathway, but Not the Insulin Receptor Substrate-1 Pathway* , 1998, The Journal of Biological Chemistry.

[4]  W. Falk,et al.  Activation of Acid Sphingomyelinase by Interleukin-1 (IL-1) Requires the IL-1 Receptor Accessory Protein* , 1997, The Journal of Biological Chemistry.

[5]  A. Samanta,et al.  Receptor-mediated endocytosis of IL-8: a fluorescent microscopic evidence and implication of the process in ligand-induced biological response in human neutrophils. , 1997, Cytokine.

[6]  B. Aggarwal,et al.  Early events in TNF signaling: a story of associations and dissociations , 1997, Journal of leukocyte biology.

[7]  R. Kolesnick,et al.  Kinase Suppressor of Ras Is Ceramide-Activated Protein Kinase , 1997, Cell.

[8]  P. Scheurich,et al.  Membrane Tumor Necrosis Factor (TNF) Induced Cooperative Signaling of TNFR60 and TNFR80 Favors Induction of Cell Death Rather Than Virus Production in HIV-infected T Cells , 1997, The Journal of experimental medicine.

[9]  J. Farber,et al.  The Cytotoxicity of Tumor Necrosis Factor Depends on Induction of the Mitochondrial Permeability Transition* , 1996, The Journal of Biological Chemistry.

[10]  Michael Karin,et al.  Ultraviolet Light and Osmotic Stress: Activation of the JNK Cascade Through Multiple Growth Factor and Cytokine Receptors , 1996, Science.

[11]  David Baltimore,et al.  An Essential Role for NF-κB in Preventing TNF-α-Induced Cell Death , 1996, Science.

[12]  Michael Karin,et al.  Dissection of TNF Receptor 1 Effector Functions: JNK Activation Is Not Linked to Apoptosis While NF-κB Activation Prevents Cell Death , 1996, Cell.

[13]  J. Schneider-Mergener,et al.  FAN, a Novel WD-Repeat Protein, Couples the p55 TNF-Receptor to Neutral Sphingomyelinase , 1996, Cell.

[14]  D. Adam,et al.  A Novel Cytoplasmic Domain of the p55 Tumor Necrosis Factor Receptor Initiates the Neutral Sphingomyelinase Pathway* , 1996, The Journal of Biological Chemistry.

[15]  David Wallach,et al.  Involvement of MACH, a Novel MORT1/FADD-Interacting Protease, in Fas/APO-1- and TNF Receptor–Induced Cell Death , 1996, Cell.

[16]  Matthias Mann,et al.  FLICE, A Novel FADD-Homologous ICE/CED-3–like Protease, Is Recruited to the CD95 (Fas/APO-1) Death-Inducing Signaling Complex , 1996, Cell.

[17]  D. Goeddel,et al.  TNF-dependent recruitment of the protein kinase RIP to the TNF receptor-1 signaling complex. , 1996, Immunity.

[18]  B. Aggarwal,et al.  Tumor necrosis factors: developments during the last decade. , 1996, European cytokine network.

[19]  A. Chinnaiyan,et al.  FADD/MORT1 Is a Common Mediator of CD95 (Fas/APO-1) and Tumor Necrosis Factor Receptor-induced Apoptosis (*) , 1996, The Journal of Biological Chemistry.

[20]  A. Samanta,et al.  Dansyl cadaverine regulates ligand induced endocytosis of interleukin‐8 receptor in human polymorphonuclear neutrophils , 1996, FEBS Letters.

[21]  George Kollias,et al.  The transmembrane form of tumor necrosis factor is the prime activating ligand of the 80 kDa tumor necrosis factor receptor , 1995, Cell.

[22]  Richard G. W. Anderson,et al.  Compartmentalized Production of Ceramide at the Cell Surface (*) , 1995, The Journal of Biological Chemistry.

[23]  J. Jaffrezou,et al.  Low Temperatures and Hypertonicity Do Not Block Cytokine-induced Stimulation of the Sphingomyelin Pathway but Inhibit Nuclear Factor-κB Activation (*) , 1995, The Journal of Biological Chemistry.

[24]  D. Goeddel,et al.  TRAF2-mediated activation of NF-kappa B by TNF receptor 2 and CD40 , 1995, Science.

[25]  T. Mak,et al.  CD28 signals through acidic sphingomyelinase , 1995, The Journal of experimental medicine.

[26]  P. Leder,et al.  RIP: A novel protein containing a death domain that interacts with Fas/APO-1 (CD95) in yeast and causes cell death , 1995, Cell.

[27]  D. Green,et al.  The end of the (cell) line: methods for the study of apoptosis in vitro. , 1995, Methods in cell biology.

[28]  H. K. Sluss,et al.  Signal transduction by tumor necrosis factor mediated by JNK protein kinases , 1994, Molecular and cellular biology.

[29]  Y. Rikihisa,et al.  Inhibition of infection of macrophages with Ehrlichia risticii by cytochalasins, monodansylcadaverine, and taxol , 1994, Infection and immunity.

[30]  M. Krönke,et al.  Functional dichotomy of neutral and acidic sphingomyelinases in tumor necrosis factor signaling , 1994, Cell.

[31]  P. Scheurich,et al.  TNF receptors TR60 and TR80 can mediate apoptosis via induction of distinct signal pathways. , 1994, Journal of immunology.

[32]  R. Heller,et al.  Tumor necrosis factor receptor-mediated signaling pathways , 1994, The Journal of cell biology.

[33]  B. Aggarwal,et al.  TNF induces internalization of the p60 receptor and shedding of the p80 receptor. , 1994, Journal of immunology.

[34]  W. Fiers,et al.  Molecular mechanisms of tumor necrosis factor‐induced cytotoxicity , 1994, FEBS letters.

[35]  F. Porteu,et al.  Tumor necrosis factor induces a selective shedding of its p75 receptor from human neutrophils. , 1994, The Journal of biological chemistry.

[36]  R. Kolesnick,et al.  Renaturation and tumor necrosis factor-alpha stimulation of a 97-kDa ceramide-activated protein kinase. , 1994, The Journal of biological chemistry.

[37]  L. Tartaglia,et al.  Stimulation of human T-cell proliferation by specific activation of the 75-kDa tumor necrosis factor receptor. , 1993, Journal of immunology.

[38]  R. Kolesnick,et al.  Substrate recognition by ceramide-activated protein kinase. Evidence that kinase activity is proline-directed. , 1993, The Journal of biological chemistry.

[39]  Wei Li,et al.  Tumor necrosis factor-induced activation and increased tyrosine phosphorylation of mitogen-activated protein (MAP) kinase in human fibroblasts. , 1993, The Journal of biological chemistry.

[40]  W. Fiers,et al.  Depletion of the mitochondrial electron transport abrogates the cytotoxic and gene‐inductive effects of TNF. , 1993, The EMBO journal.

[41]  R. Kolesnick,et al.  Sphingomyelinase and ceramide activate mitogen-activated protein kinase in myeloid HL-60 cells. , 1993, The Journal of biological chemistry.

[42]  T. Mak,et al.  Mice deficient for the 55 kd tumor necrosis factor receptor are resistant to endotoxic shock, yet succumb to L. monocytogenes infection , 1993, Cell.

[43]  Mike Rothe,et al.  Tumor necrosis factor's cytotoxic activity is signaled by the p55 TNF receptor , 1993, Cell.

[44]  M. Krönke,et al.  TNF activates NF-κB by phosphatidylcholine-specific phospholipase C-induced “Acidic” sphingomyelin breakdown , 1992, Cell.

[45]  M. Krönke,et al.  Human 55-kDa receptor for tumor necrosis factor coupled to signal transduction cascades. , 1992, The Journal of biological chemistry.

[46]  R. Gentz,et al.  Both tumor necrosis factor receptor types mediate proliferative signals in human mononuclear cell activation. , 1992, Journal of immunology.

[47]  C. Unger,et al.  Tumor necrosis factor induces rapid production of 1'2'diacylglycerol by a phosphatidylcholine-specific phospholipase C , 1991, The Journal of experimental medicine.

[48]  W. Fiers.  Tumor necrosis factor Characterization at the molecular, cellular and in vivo level , 1991, FEBS letters.

[49]  F. Balkwill.  Cytokines : a practical approach , 1991 .

[50]  E. Weber,et al.  Internalization of endothelin by cultured human vascular smooth muscle cells: characterization and physiological significance. , 1990, Molecular pharmacology.

[51]  K. Pfizenmaier,et al.  Tumor necrosis factor signal transduction. Cell-type-specific activation and translocation of protein kinase C. , 1990, Journal of immunology.

[52]  K. Matsushima,et al.  Interleukin 8 (monocyte-derived neutrophil chemotactic factor) dynamically regulates its own receptor expression on human neutrophils. , 1990, The Journal of biological chemistry.

[53]  H. Kuriyama,et al.  Continuous internalization of tumor necrosis factor receptors in a human myosarcoma cell line. , 1988, The Journal of biological chemistry.

[54]  B. Beutler,et al.  Cachectin and tumour necrosis factor as two sides of the same biological coin , 1986, Nature.

[55]  D. Goeddel,et al.  Tumor necrosis factors: gene structure and biological activities. , 1986, Cold Spring Harbor symposia on quantitative biology.

[56]  W. Duckworth,et al.  Selective effects of inhibitors of hormone processing on insulin action in isolated hepatocytes. , 1984, Endocrinology.

[57]  Richard G. W. Anderson,et al.  Depletion of intracellular potassium arrests coated pit formation and receptor-mediated endocytosis in fibroblasts , 1983, Cell.

[58]  I. Pastan,et al.  Amantadine and dansylcadaverine inhibit vesicular stomatitis virus uptake and receptor-mediated endocytosis of alpha 2-macroglobulin. , 1982, Proceedings of the National Academy of Sciences of the United States of America.

[59]  P. Cuatrecasas,et al.  Possible requirement of internalization in the mechanism of in vitro cytotoxicity in tumor necrosis serum. , 1981, Cancer research.

[60]  I. Pastan,et al.  Dansylcadaverine inhibits internalization of 125I-epidermal growth factor in BALB 3T3 cells. , 1980, The Journal of biological chemistry.

[61]  I. Pastan,et al.  Transglutaminase is essential in receptor-mediated endocytosis of α2-macroglobulin and polypeptide hormones , 1980, Nature.

[62]  I. Pastan,et al.  Amines inhibit the clustering of α2-macroglobulin and EGF on the fibroblast cell surface , 1979, Nature.

[63]  J. E. Folk,et al.  Molecular and catalytic properties of transglutaminases. , 1973, Advances in enzymology and related areas of molecular biology.