Lactobacillus johnsonii La1 shares carbohydrate-binding specificities with several enteropathogenic bacteria.

The carbohydrate-binding specificities of the probiotic lactic acid bacterium Lactobacillus johnsonii La1 (a health-beneficial bacterial strain able to be incorporated into the human intestinal microflora) were investigated in vitro. First various soluble complex carbohydrates were tested as potential inhibitors of the strain adhesion onto Caco-2 intestinal epithelial cells, and then bacterial binding to glycolipids immobilized on TLC plates was probed. Two major carbohydrate-binding specificities of Lactobacillus johnsonii La1 were identified. A first one for an Endo-H treated yeast cell wall mannoprotein carrying mainly O:-linked oligomannosides, and a second one for the gangliotri- and gangliotetra-osylceramides (asialo-GM1). Similar carbohydrate-binding specificities are known to be expressed on cell surface adhesins of several enteropathogens, enabling them to adhere to the host gut mucosa. These findings corroborate the hypothesis that selected probiotic bacterial strains could be able to compete with enteropathogens for the same carbohydrate receptors in the gut.

[1]  W. Hammes,et al.  Activation of Human Peripheral Blood Mononuclear Cells by Nonpathogenic Bacteria In Vitro: Evidence of NK Cells as Primary Targets , 2000, Infection and Immunity.

[2]  D. Brassart,et al.  Cell Surface-Associated Lipoteichoic Acid Acts as an Adhesion Factor for Attachment of Lactobacillus johnsoniiLa1 to Human Enterocyte-Like Caco-2 Cells , 1999, Applied and Environmental Microbiology.

[3]  D. Brassart,et al.  The use of probiotics to reinforce mucosal defence mechanisms , 1997 .

[4]  D. Brassart,et al.  The human Lactobacillus acidophilus strain LA1 secretes a nonbacteriocin antibacterial substance(s) active in vitro and in vivo , 1997, Applied and environmental microbiology.

[5]  B. Finlay,et al.  Exploitation of mammalian host cell functions by bacterial pathogens. , 1997, Science.

[6]  J. Forstner,et al.  Proteinaceous factor(s) in culture supernatant fluids of bifidobacteria which prevents the binding of enterotoxigenic Escherichia coli to gangliotetraosylceramide , 1997, Applied and environmental microbiology.

[7]  R. Berg,et al.  The indigenous gastrointestinal microflora. , 1996, Trends in microbiology.

[8]  H. Kumagai,et al.  Binding Specificity ofLactobacillusto Glycolipids , 1996 .

[9]  S. Ahrné,et al.  A mannose-specific adherence mechanism in Lactobacillus plantarum conferring binding to the human colonic cell line HT-29 , 1996, Applied and environmental microbiology.

[10]  S. Salminen,et al.  The coming of age of probiotics , 1995 .

[11]  N. Kalkkinen,et al.  A Collagen-Binding S-Layer Protein in Lactobacillus crispatus , 1995, Applied and environmental microbiology.

[12]  J. Roth,et al.  Virulence Mechanisms of Bacterial Pathogens , 1995 .

[13]  E. Schiffrin,et al.  Immunomodulation of human blood cells following the ingestion of lactic acid bacteria. , 1995, Journal of dairy science.

[14]  C. Wennerås,et al.  Binding of the fibrillar CS3 adhesin of enterotoxigenic Escherichia coli to rabbit intestinal glycoproteins is competitively prevented by GalNAc beta 1-4Gal-containing glycoconjugates , 1995, Infection and immunity.

[15]  F. Rochat,et al.  Modulation of a specific humoral immune response and changes in intestinal flora mediated through fermented milk intake. , 1994, FEMS immunology and medical microbiology.

[16]  P. Klemm.  Fimbriae Adhesion, Genetics, Biogenesis, and Vaccines , 1994 .

[17]  D. Brassart,et al.  Lactobacillus acidophilus LA 1 binds to cultured human intestinal cell lines and inhibits cell attachment and cell invasion by enterovirulent bacteria. , 1994, Gut.

[18]  R. Hodges,et al.  The binding of Pseudomonas aeruginosa pili to glycosphingolipids is a tip‐associated event involving the C‐terminal region of the structural pilin subunit , 1994, Molecular microbiology.

[19]  B. Finlay,et al.  Enteropathogenic Escherichia coli decreases the transepithelial electrical resistance of polarized epithelial monolayers , 1993, Infection and immunity.

[20]  A. Surolia,et al.  Identification of carbohydrate structures as receptors for localised adherent enteropathogenic Escherichia coli. , 1991, Microbial pathogenesis.

[21]  B. Finlay,et al.  Salmonella interactions with polarized human intestinal Caco-2 epithelial cells. , 1990, The Journal of infectious diseases.

[22]  A. Kolstø,et al.  Identification of asialo GM1 as a binding structure for Escherichia coli colonization factor antigens , 1990 .

[23]  J. Neeser,et al.  Adhesion of colonization factor antigen II-positive enterotoxigenic Escherichia coli strains to human enterocytelike differentiated HT-29 cells: a basis for host-pathogen interactions in the gut , 1989, Infection and immunity.

[24]  D. Roberts,et al.  Many pulmonary pathogenic bacteria bind specifically to the carbohydrate sequence GalNAc beta 1-4Gal found in some glycolipids. , 1988, Proceedings of the National Academy of Sciences of the United States of America.

[25]  G. Teti,et al.  Adherence of group B streptococci to adult and neonatal epithelial cells mediated by lipoteichoic acid , 1987, Infection and immunity.

[26]  G. Teti,et al.  Mediation of Staphylococcus saprophyticus adherence to uroepithelial cells by lipoteichoic acid , 1987, Infection and immunity.

[27]  D. Savage,et al.  Lipoteichoic acids in Lactobacillus strains that colonize the mouse gastric epithelium , 1986, Applied and environmental microbiology.

[28]  A. Dell,et al.  Characterization of acetylated and acetolyzed glycoprotein high-mannose core oligosaccharides by fast-atom-bombardment mass spectrometry. , 1986, Proceedings of the National Academy of Sciences of the United States of America.

[29]  B. Koellreutter,et al.  Oligomannoside-type glycopeptides inhibiting adhesion of Escherichia coli strains mediated by type 1 pili: preparation of potent inhibitors from plant glycoproteins , 1986, Infection and immunity.

[30]  J. Feeney,et al.  Structural analysis of the O-glycosidically linked core-region oligosaccharides of human meconium glycoproteins which express oncofoetal antigens. , 1985, European journal of biochemistry.

[31]  K. Karlsson,et al.  Carbohydrate-specific adhesion of bacteria to thin-layer chromatograms: a rationalized approach to the study of host cell glycolipid receptors. , 1985, Analytical biochemistry.

[32]  C. Ballou,et al.  Saccharomyces cerevisiae structural cell wall mannoprotein. , 1985, Biochemistry.

[33]  N. Firon,et al.  Carbohydrate specificity of the surface lectins of Escherichia coli, Klebsiella pneumoniae, and Salmonella typhimurium. , 1983, Carbohydrate research.

[34]  J. Prestegard,et al.  High-resolution proton NMR studies of gangliosides. 1. Use of homonuclear two-dimensional spin-echo J-correlated spectroscopy for determination of residue composition and anomeric configurations. , 1983, Biochemistry.

[35]  E. Beachey,et al.  Epithelial cell binding of group A streptococci by lipoteichoic acid on fimbriae denuded of M protein , 1976, The Journal of experimental medicine.

[36]  E. Beachey,et al.  Cell membrane-binding properties of group A streptococcal lipoteichoic acid , 1975, The Journal of experimental medicine.

[37]  S. Hakomori,et al.  Determination of aminosugar linkages in glycolipids by methylation. Aminosugar linkages of ceramide pentasaccharides of rabbit erythrocytes and of Forssman antigen. , 1973, Archives of biochemistry and biophysics.

[38]  S. Hakomori,et al.  A sphingolipid having a novel type of ceramide and lacto-N-fucopentaose 3. , 1971, The Journal of biological chemistry.

[39]  M. Bennett,et al.  Murine natural killer cells and marrow graft rejection. , 1992, Annual review of immunology.

[40]  K. Karlsson,et al.  Mass spectrometry of mixtures of intact glycosphingolipids. , 1990, Methods in enzymology.

[41]  K. Karlsson.  Animal glycosphingolipids as membrane attachment sites for bacteria. , 1989, Annual review of biochemistry.

[42]  L. Payne,et al.  Protein antigen-monoclonal antibody contact sites investigated by limited proteolysis of monoclonal antibody-bound antigen: protein "footprinting". , 1988, Proceedings of the National Academy of Sciences of the United States of America.

[43]  K. Karlsson.  Preparation of total nonacid glycolipids for overlay analysis of receptors for bacteria and viruses and for other studies. , 1987, Methods in enzymology.

[44]  M. Pinto,et al.  Enterocyte-like differentiation and polarization of the human colon carcinoma cell line Caco-2 in culture , 1983 .

[45]  C. Ballou.  Yeast Cell Wall and Cell Surface , 1982 .

[46]  J. Broach,et al.  The Molecular biology of the yeast Saccharomyces : metabolism and gene expression , 1982 .

[47]  K. Karlsson,et al.  Proton nuclear magnetic resonance analysis of anomeric structure of glycosphingolipids. The globo-series (one to five sugars). , 1979, Archives of biochemistry and biophysics.

[48]  K. Karlsson,et al.  Proton nuclear magnetic resonance analysis of anomeric structure of glycosphingolipids. Blood group ABH-active substances. , 1979, Archives of biochemistry and biophysics.