Identification of resistance gene analogue markers closely linked to wheat powdery mildew resistance gene Pm31

Specific oligonucleotide primers, designed for the sequences of known plant disease resistance genes, were used to amplify resistance gene analogues (RGAs) from wheat genomic DNA. This method was applied in a bulked segregant analysis to screen for the RGA markers linked to the powdery mildew resistance gene Pm31, introgressed into common wheat from wild emmer. Two RGA markers (RGA200 and RGA390) were found to be closely linked to Pm31 and completely co-segregating with the marker allele of Xpsp3029 linked to Pm31, with a genetic distance of 0.6 cM. These two RGA markers were then integrated into the formerly established microsatellite map of Pm31 region. The result showed the effectiveness of the RGA approach for developing molecular markers linked to disease resistance genes and demonstrated the efficiency of denaturing polyacrylamide-gel electrophoresis for detecting polymerase chain reaction polymorphism.

[1]  M. Feldman,et al.  A new powdery mildew resistance gene: Introgression from wild emmer into common wheat and RFLP-based mapping , 2000, Euphytica.

[2]  E. Nevo,et al.  Molecular characterization of a novel powdery mildew resistance gene Pm30 in wheat originating from wild emmer , 2004, Euphytica.

[3]  A. Adam-Blondon,et al.  Identification of resistance gene analogs linked to a powdery mildew resistance locus in grapevine , 2002, Theoretical and Applied Genetics.

[4]  E. Nevo,et al.  Chromosomal location of a Triticum dicoccoides-derived powdery mildew resistance gene in common wheat by using microsatellite markers , 2002, Theoretical and Applied Genetics.

[5]  H. Peusha,et al.  Chromosomal location of powdery mildew resistance genes and cytogenetic analysis of meiosis in common wheat cultivar Meri. , 2004, Hereditas.

[6]  A. Laroche,et al.  NBS-LRR sequence family is associated with leaf and stripe rust resistance on the end of homoeologous chromosome group 1S of wheat , 2000, Theoretical and Applied Genetics.

[7]  K. Devos,et al.  Chromosomal location of a Triticum timopheevii - derived powdery mildew resistance gene transferred to common wheat , 2000 .

[8]  H. Leung,et al.  Genome scanning for resistance-gene analogs in rice, barley, and wheat by high-resolution electrophoresis , 1998, Theoretical and Applied Genetics.

[9]  Jonathan D. G. Jones,et al.  PLANT DISEASE RESISTANCE GENES. , 1997, Annual review of plant physiology and plant molecular biology.

[10]  B. Keller,et al.  Molecular cloning of a new receptor-like kinase gene encoded at the Lr10 disease resistance locus of wheat. , 1997, The Plant journal : for cell and molecular biology.

[11]  Y. G. Yu,et al.  Isolation of a superfamily of candidate disease-resistance genes in soybean based on a conserved nucleotide-binding site. , 1996, Proceedings of the National Academy of Sciences of the United States of America.

[12]  M. Daly,et al.  MAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. , 1987, Genomics.

[13]  R. Jorgensen,et al.  Ribosomal DNA spacer-length polymorphisms in barley: mendelian inheritance, chromosomal location, and population dynamics. , 1984, Proceedings of the National Academy of Sciences of the United States of America.