Physical and mechanical properties of the fully interconnected chitosan ice‐templated scaffolds

Porous chitosan scaffolds were prepared with a freeze-casting technique with different concentrations, 1.5 and 3 wt %, and also different cooling rates, 1 and 4 � C/min. The pore morphology, porosity, pore size, mechanical properties, and water absorp- tion characteristics of the scaffolds were studied. Scanning electron microscopy images showed that the freeze-cast scaffolds were fully interconnected because of the existence of pores on the chitosan walls in addition to many unidirectionally elongated pores. Increases in the chitosan concentration and freezing rate led to elevations in the thickness of the chitosan walls and reductions in the pores size, respectively. These two results led to the enhancement of the compressive strength from 34 to 110 kPa for the scaffolds that had 96-98% porosity. Also, augmentation of the chitosan concentration and decreases in the freezing rate led to the reduction of the number of pores on the chitosan walls. Furthermore, the volume of water absorption increased with a reduction in the chitosan con- centration and cooling rate from 690 to 1020%. V C 2014 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2015, 132, 41476.

[1]  A. Zamanian,et al.  Effect of cooling rate and gelatin concentration on the microstructural and mechanical properties of ice template gelatin scaffolds , 2013, Biotechnology and applied biochemistry.

[2]  Changren Zhou,et al.  Chitosan-halloysite nanotubes nanocomposite scaffolds for tissue engineering. , 2013, Journal of materials chemistry. B.

[3]  M. Elsabee,et al.  Chitosan based nanofibers, review. , 2012, Materials science & engineering. C, Materials for biological applications.

[4]  Wenle Li,et al.  Freeze casting of porous materials: review of critical factors in microstructure evolution , 2012 .

[5]  S. Mishra,et al.  Organic-inorganic hybrid of chitosan/organoclay bionanocomposites for hexavalent chromium uptake. , 2011, Journal of colloid and interface science.

[6]  Jonghwi Lee,et al.  Chitosan fibrous 3D networks prepared by freeze drying , 2011 .

[7]  A. Khademhosseini,et al.  Fabrication of porous chitosan scaffolds for soft tissue engineering using dense gas CO2. , 2011, Acta biomaterialia.

[8]  Yunfeng Shi,et al.  Preparation, structure and crystallinity of chitosan nano-fibers by a solid-liquid phase separation technique , 2011 .

[9]  S. Deville.  Freeze-Casting of Porous Biomaterials: Structure, Properties and Opportunities , 2010, Materials.

[10]  Jiecai Han,et al.  Camphene-based freeze-cast ZrO2 foam with high compressive strength , 2010 .

[11]  S. Nair,et al.  Nanocomposite scaffolds of bioactive glass ceramic nanoparticles disseminated chitosan matrix for tissue engineering applications , 2010 .

[12]  J. Covington,et al.  Conducting Nanocomposite Polymer Foams from Ice‐Crystal‐Templated Assembly of Mixtures of Colloids , 2009 .

[13]  Hyoun‐Ee Kim,et al.  Fabrication of porous titanium scaffolds with high compressive strength using camphene-based freeze casting , 2009 .

[14]  Jiecai Han,et al.  Ultra-high-porosity zirconia ceramics fabricated by novel room-temperature freeze-casting , 2009 .

[15]  Z. Cui,et al.  Collagen–chitosan polymer as a scaffold for the proliferation of human adipose tissue-derived stem cells , 2009, Journal of materials science. Materials in medicine.

[16]  G. Wallace,et al.  Characterisation of porous freeze dried conducting carbon nanotube–chitosan scaffolds , 2008 .

[17]  S. Deville.  Freeze‐Casting of Porous Ceramics: A Review of Current Achievements and Issues , 2008, 1710.04201.

[18]  M. Gutiérrez,et al.  Ice-Templated Materials: Sophisticated Structures Exhibiting Enhanced Functionalities Obtained after Unidirectional Freezing and Ice-Segregation-Induced Self-Assembly† , 2008 .

[19]  L. Gibson,et al.  Mechanical characterization of collagen-glycosaminoglycan scaffolds. , 2007, Acta biomaterialia.

[20]  Hui Yang,et al.  Gel-casting without de-airing process using silica sol as a binder , 2007 .

[21]  Eduardo Saiz,et al.  Freeze casting of hydroxyapatite scaffolds for bone tissue engineering. , 2006, Biomaterials.

[22]  Makarand V Risbud,et al.  Chitosan: a versatile biopolymer for orthopaedic tissue-engineering. , 2005, Biomaterials.

[23]  O. Kwon,et al.  Electrospinning of chitosan dissolved in concentrated acetic acid solution. , 2005, Biomaterials.

[24]  D. Kaplan,et al.  Porosity of 3D biomaterial scaffolds and osteogenesis. , 2005, Biomaterials.

[25]  Miqin Zhang,et al.  Chitosan-alginate hybrid scaffolds for bone tissue engineering. , 2005, Biomaterials.

[26]  T. Hsien,et al.  Preparation and characterization of RGD-immobilized chitosan scaffolds. , 2005, Biomaterials.

[27]  Mingzhong Li,et al.  Preparation and Structure of Porous Silk Sericin Materials , 2005 .

[28]  L. Gibson,et al.  The effect of pore size on cell adhesion in collagen-GAG scaffolds. , 2005, Biomaterials.

[29]  S. Semiatin,et al.  Thermal Conductivity of Plasma-Sprayed Monolithic and Multilayer Coatings of Alumina and Yttria-Stabilized Zirconia , 2004 .

[30]  M. Tuszynski,et al.  The fabrication and characterization of linearly oriented nerve guidance scaffolds for spinal cord injury. , 2004, Biomaterials.

[31]  A R Boccaccini,et al.  Porous poly(alpha-hydroxyacid)/Bioglass composite scaffolds for bone tissue engineering. I: Preparation and in vitro characterisation. , 2004, Biomaterials.

[32]  Jiang Chang,et al.  Preparation and characterization of macroporous chitosan/wollastonite composite scaffolds for tissue engineering , 2004, Journal of materials science. Materials in medicine.

[33]  Lie Ma,et al.  Collagen/chitosan porous scaffolds with improved biostability for skin tissue engineering. , 2003, Biomaterials.

[34]  Eugene Khor,et al.  Implantable applications of chitin and chitosan. , 2003, Biomaterials.

[35]  Bin Wu,et al.  Evaluation of the biocompatibility of a chitosan scaffold in mice. , 2002, Journal of biomedical materials research.

[36]  Scott J Hollister,et al.  Mechanical and in vivo performance of hydroxyapatite implants with controlled architectures. , 2002, Biomaterials.

[37]  D. Hutmacher,et al.  Scaffolds in tissue engineering bone and cartilage. , 2000, Biomaterials.

[38]  J. Leroux,et al.  Novel injectable neutral solutions of chitosan form biodegradable gels in situ. , 2000, Biomaterials.

[39]  J. Leroux,et al.  Characterization of thermosensitive chitosan gels for the sustained delivery of drugs. , 2000, International journal of pharmaceutics.

[40]  I. Heschel,et al.  Dendritic ice morphology in unidirectionally solidified collagen suspensions , 2000 .

[41]  S. Madihally,et al.  Porous chitosan scaffolds for tissue engineering. , 1999, Biomaterials.

[42]  H. Kaş.  Chitosan: properties, preparations and application to microparticulate systems. , 1997, Journal of microencapsulation.

[43]  Keshun Liu,et al.  Soybeans: Chemistry, Technology and Utilization , 1997 .

[44]  R. Muzzarelli,et al.  Stimulatory effect on bone formation exerted by a modified chitosan. , 1994, Biomaterials.

[45]  Jiecai Han,et al.  Highly porous ZrO2 ceramics fabricated by a camphene-based freeze-casting route: Microstructure and properties , 2010 .

[46]  L. A. Genova,et al.  Effect of starch filler content and sintering temperature on the processing of porous 3Y–ZrO2 ceramics , 2009 .

[47]  In-Yong Kim,et al.  Chitosan and its derivatives for tissue engineering applications. , 2008, Biotechnology advances.

[48]  Juin-Yih Lai,et al.  Preparation of porous scaffolds by using freeze-extraction and freeze-gelation methods. , 2004, Biomaterials.

[49]  J M Powers,et al.  Fabrication of biodegradable polymer scaffolds to engineer trabecular bone. , 1995, Journal of biomaterials science. Polymer edition.