Voltammetric determination of binding constant and stoichiometry of albumin (human, bovine, ovine) - drugs complexes.

The parameters characterizing the formation of complexes with albumin (in particular, human serum albumin - HSA) are fundamental for the characterization of a drug for commercialization purposes and for the determination of common pharmacokinetic parameters. Electrochemical methods appear particularly attractive for the determination of the complexation constant, complex stoichiometry, and percentage of free/bound drug, due to the ease of operation and the wide availability. In this article, we propose an electrochemical method based on differential pulse voltammetry for the determination of albumin - drugs interaction parameters, including the replacement of the drug - albumin adduct by a competitive compound, namely sulfanilamide. The formation of either single or multiple complexes between the considered drug and albumin has been considered. Typically, the method operates with a glassy carbon electrode in NaCl 0.9% as the supporting electrolyte.

[1]  E. Ngameni,et al.  Voltammetric behavior of Mammeisin (MA) at a glassy carbon electrode and its interaction with Bovine Serum Albumin (BSA). , 2018, Bioelectrochemistry.

[2]  S. Mitra,et al.  Caffeine and sulfadiazine interact differently with human serum albumin: A combined fluorescence and molecular docking study. , 2016, Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy.

[3]  N. Rajendiran,et al.  Interaction of sulfanilamide and sulfamethoxazole with bovine serum albumin and adenine: spectroscopic and molecular docking investigations. , 2015, Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy.

[4]  Bin Liu,et al.  Spectroscopic investigation on the food components-drug interaction: the influence of flavonoids on the affinity of nifedipine to human serum albumin. , 2015, Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association.

[5]  C. Wanner,et al.  Clearance of drugs for multiple myeloma therapy during in vitro high-cutoff hemodialysis. , 2014, Artificial organs.

[6]  Jingwei Shao,et al.  Compilation of 222 drugs' plasma protein binding data and guidance for study designs. , 2012, Drug discovery today.

[7]  Xiangjing Wang,et al.  Comparative studies on the interaction of genistein, 8-chlorogenistein, and 3',8-dichlorogenistein with bovine serum albumin. , 2011, Journal of agricultural and food chemistry.

[8]  Shilpi Agarwal,et al.  Voltammetric techniques for the assay of pharmaceuticals--a review. , 2011, Analytical biochemistry.

[9]  L. Bruzzone,et al.  Binding of several benzodiazepines to bovine serum albumin: Fluorescence study. , 2010, Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy.

[10]  Julie Schappler,et al.  Drug–protein binding: a critical review of analytical tools , 2010, Analytical and bioanalytical chemistry.

[11]  M. L. Howard,et al.  Plasma protein binding in drug discovery and development. , 2010, Combinatorial chemistry & high throughput screening.

[12]  You-nian Liu,et al.  Estimation of Binding Constants for Diclofenac Sodium and Bovine Serum Albumin by Affinity Capillary Electrophoresis and Fluorescence Spectroscopy , 2008 .

[13]  Yi Liu,et al.  Interaction of rofecoxib with human serum albumin: Determination of binding constants and the binding site by spectroscopic methods , 2008 .

[14]  G. Trainor,et al.  The importance of plasma protein binding in drug discovery , 2007, Expert opinion on drug discovery.

[15]  Wei Sun,et al.  Linear sweep voltammetric studies on the interaction of brilliant cresyl blue with nucleic acids and its analytical application. , 2006, Annali di chimica.

[16]  Jahan B. Ghasemi,et al.  Determination of Trace Amounts of Lorazepam by Adsorptive Cathodic Differential Pulse Stripping Method in Pharmaceutical Formulations and Biological Fluids , 2006 .

[17]  Enzo Terreno,et al.  The extraordinary ligand binding properties of human serum albumin , 2005, IUBMB life.

[18]  Hongyuan Chen,et al.  Determination of Binding Constants for Basic Drugs with Serum Albumin by Affinity Capillary Electrophoresis with the Partial Filling Technique , 2005 .

[19]  J. Mendes,et al.  Evolution of the thyroid hormone-binding protein, transthyretin. , 2000, General and comparative endocrinology.

[20]  G Houin,et al.  Diclofenac binding to albumin and lipoproteins in human serum. , 1985, Biochemical pharmacology.

[21]  J. Vallner.  Binding of drugs by albumin and plasma protein. , 1977, Journal of pharmaceutical sciences.

[22]  Hanqi Zhang,et al.  Investigation of ketoprofen binding to human serum albumin by spectral methods. , 2011, Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy.

[23]  Y. Ni,et al.  Spectrometric and voltammetric studies of the interaction between quercetin and bovine serum albumin using warfarin as site marker with the aid of chemometrics. , 2009, Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy.

[24]  Wei Sun,et al.  Studies on the interaction of protein with acid chrome blue K by electrochemical method and its analytical application. , 2006, Bioelectrochemistry.