Bio-medical waste management rules were formulated in response to the worldwide public concern over medical waste. The practice of separation into different types of waste in health care institutes should be evaluated more scientifically. Due to a lack of data from the Indian sub-continent, this study was initiated at a tertiary care hospital. Samples were collected from different types of waste at the hospital, at different time intervals, for microbiological evaluation. The results reveal that the microbial flora isolated from infectious waste and general waste from the hospital are similar. The samples from general waste in this study reveal many types of pathogens. The bacteria present in the waste initially was low in quantity, but they replicated rapidly over time so that significant numbers were detected by 24 hours, due to environmental factors which were favorable for growth during this period. This study strongly suggests that waste should be removed from the hospital within 24 hours of its generation to prevent environmental contamination caused by any accidental spillage of waste. General waste generated in the hospital should be treated similar to infectious waste, as it can be equally hazardous.
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