In vitro Antihyperglycemic and Chelating Potential of Selected Ayurvedic Medicinal Plants

Seven medicinal plants used in folk medicine to relieve various diseases including diabetes, were investigated for antihyperglycemic activities. Two types of extracts for each plant were processed by decoction and by soaking in an aqueous-acetone mixture. The inhibitory activities were tested on α-amylase and α-glucosidase along with chelating activity. The aqueous-acetone extracts of Feretia apodanthera and Ozoroa insignis exerted strong inhibition on α-glucosidase (96.51±0.14 and 96.44±0.68 %, respectively) but lower inhibition on α-amylase (21.60±1.69 and 93.85±6.58 %, respectively) and displayed a weak chelating activity as compared to the respective standards acarbose and ethylenediaminetetraacetic acid. Subsequent column fractionation resulted in selectively strong inhibition of α-glucosidase. The inhibitory activities of α-amylase and α-glucosidase were weakly correlated (R=0,389) and both were inversely correlated with metal chelating activity (R=-0.731 and R=-0.809, respectively). Volatile compounds, 1-nonadecene and 1-heptadecene identified by GC-MS were related to the inhibitory activities of α-amylase and α-glucosidase enzymes. This potent inhibition of sugar degrading enzymes may help to manage the hyperglycemia occurring in type 2 diabetes.

[1]  Mengtao Zhou,et al.  High Glucose-Induced ROS Production Stimulates Proliferation of Pancreatic Cancer via Inactivating the JNK Pathway , 2018, Oxidative medicine and cellular longevity.

[2]  Duangjai Tungmunnithum,et al.  Flavonoids and Other Phenolic Compounds from Medicinal Plants for Pharmaceutical and Medical Aspects: An Overview , 2018, Medicines.

[3]  Shin-Hye Kim,et al.  Effects of growth hormone on glucose metabolism and insulin resistance in human , 2017, Annals of pediatric endocrinology & metabolism.

[4]  D. Mears,et al.  Glucose-Dependent Insulin Secretion in Pancreatic β-Cell Islets from Male Rats Requires Ca2+ Release via ROS-Stimulated Ryanodine Receptors , 2015, PloS one.

[5]  S. Sulaiman,et al.  Bioprospecting medicinal plants for antioxidant components. , 2014, Asian Pacific journal of tropical medicine.

[6]  T. Das,et al.  Oxidative Stress Gated by Fenton and Haber Weiss Reactions and Its Association With Alzheimer’s Disease , 2014 .

[7]  A. Verberne,et al.  Neural pathways that control the glucose counterregulatory response , 2014, Front. Neurosci..

[8]  S. Sulaiman,et al.  Antioxidant and α-glucosidase inhibitory activities of cucurbit fruit vegetables and identification of active and major constituents from phenolic-rich extracts of Lagenaria siceraria and Sechium edule. , 2013, Journal of agricultural and food chemistry.

[9]  U. Palanisamy,et al.  Tropical Plant Extracts as Potential Antihyperglycemic Agents , 2012, Molecules.

[10]  P. Wongsa,et al.  In vitro screening of phenolic compounds, potential inhibition against α-amylase and α-glucosidase of culinary herbs in Thailand , 2012 .

[11]  Mei Lan Tan,et al.  Cytotoxic, apoptotic and anti-α-glucosidase activities of 3,4-di-O-caffeoyl quinic acid, an antioxidant isolated from the polyphenolic-rich extract of Elephantopus mollis Kunth. , 2011, Journal of ethnopharmacology.

[12]  S. Zinjarde,et al.  Potent α-amylase inhibitory activity of Indian Ayurvedic medicinal plants , 2011, BMC complementary and alternative medicine.

[13]  J. Crandall,et al.  Type 2 Diabetes Prevention: A Review , 2010, Clinical Diabetes.

[14]  F. Lajolo,et al.  Potential of Ginkgo biloba L. leaves in the management of hyperglycemia and hypertension using in vitro models. , 2009, Bioresource technology.

[15]  M. Ebrahimzadeh,et al.  IRON CHELATING ACTIVITY, PHENOL AND FLAVONOID CONTENT OF SOME MEDICINAL PLANTS FROM IRAN , 2008 .

[16]  S. Withers,et al.  The Search for Novel Human Pancreatic α‐Amylase Inhibitors: High‐Throughput Screening of Terrestrial and Marine Natural Product Extracts , 2008, Chembiochem : a European journal of chemical biology.

[17]  N. Van Chuyen,et al.  Alpha-glucosidase inhibitory and antioxidant activities of Vietnamese edible plants and their relationships with polyphenol contents. , 2007, Journal of nutritional science and vitaminology.

[18]  M. Loizzo,et al.  Composition and α-amylase inhibitory effect of essential oils from Cedrus libani , 2007 .

[19]  J. Prousek.  Fenton chemistry in biology and medicine , 2007 .

[20]  M. Schubert-Zsilavecz,et al.  Screening of herbal extracts for activation of the human peroxisome proliferator-activated receptor. , 2006, Die Pharmazie.

[21]  P. Houghton,et al.  Leads from Indian medicinal plants with hypoglycemic potentials. , 2006, Journal of ethnopharmacology.

[22]  L. Pari,et al.  Antidiabetic effect of Scoparia dulcis: effect on lipid peroxidation in streptozotocin diabetes. , 2005, General physiology and biophysics.

[23]  I. G. Fantus,et al.  Oral antihyperglycemic therapy for type 2 diabetes mellitus , 2005, Canadian Medical Association Journal.

[24]  L. Pari,et al.  Protective role of Scoparia dulcis plant extract on brain antioxidant status and lipidperoxidation in STZ diabetic male Wistar rats , 2004, BMC complementary and alternative medicine.

[25]  F. Abas,et al.  Antioxidant and α-glucosidase inhibitory activities of the leaf and stem of selected traditional medicinal plants , 2014 .

[26]  K. S. D. A. S.M.Gopinath.  ANTIDIABETIC EFFECT OF A POLY HERBAL FORMULATION (D) ON HUMAN PANCREATICAMYLASE , 2013 .

[27]  M. Andjelkovic,et al.  Iron-chelation properties of phenolic acids bearing catechol and galloyl groups , 2006 .