Near Infrared and Aquaphotomic analysis of water absorption in lactate containing media

Increased concentrations of lactate levels in blood are often seen in patients with life-threatening cellular hypoperfusion or infections. State-of-the-art techniques used in clinical practice for measuring serum lactate concentrations rely on intermittent blood sampling and do not permit continuous monitoring of this all important parameter in critical care environments.In recent years, Near Infrared (NIR) Spectroscopy has been established as a possible alternative to existing methods that can mitigate these constraints and be used for non-invasive continuous monitoring of lactate. Nevertheless, the dominant absorption of -OH overtone bands of water in the NIR presents a challenge and complicates the accurate detection of other absorbers such as lactate. For this reason, comprehensive analysis of the -OH overtone bands with systematic lactate concentration changes is essential. This paper reports on the analysis of NIR spectra of two aqueous systems of varying concentrations of lactate in saline and whole blood using the principles of Aquaphotomics.The results show distinctive conformational and structural differences in lactate-water binding, which arise due to the molecular interactions of bonds present in respective solvents.

[1]  F. Caputo,et al.  Intracellular Shuttle: The Lactate Aerobic Metabolism , 2012, TheScientificWorldJournal.

[2]  Mark A. Arnold,et al.  Simultaneous Measurements of Glucose, Glutamine, Ammonia, Lactate, and Glutamate in Aqueous Solutions by Near-Infrared Spectroscopy , 1996 .

[3]  Y. Nakano,et al.  Measurement of the Concentrations of Glucose and Citric Acid in the Aqueous Solution of a Blood Anticoagulant Using near Infrared Spectroscopy , 2001 .

[4]  R. Tsenkova,et al.  Aquaphotomics—From Innovative Knowledge to Integrative Platform in Science and Technology , 2019, Molecules.

[5]  L. Lands,et al.  Near-Infrared Spectroscopic Measurement of Lactate in Human Plasma , 2000 .

[6]  Roumiana Tsenkova,et al.  Aquaphotomics: Near Infrared Spectroscopy and Water States in Biological Systems. , 2015, Sub-cellular biochemistry.

[7]  G. Lighthall,et al.  Lactate Predicts Both Short- and Long-Term Mortality in Patients With and Without Sepsis , 2019, Infectious diseases.

[8]  P. Tolstoy,et al.  Water molecules in hydroxy/acid networks as a competition between dynamics and bonding. Synthesis of a wet hydrophobic pore. , 2004, Bioorganic & medicinal chemistry.

[9]  Yuan-Chuan Tai,et al.  Penetration depth of photons in biological tissues from hyperspectral imaging in shortwave infrared in transmission and reflection geometries , 2016, Journal of biomedical optics.

[10]  Denis Lafrance,et al.  Measurement of lactate in whole human blood with near-infrared transmission spectroscopy. , 2003, Talanta.

[11]  D. Pang,et al.  Lactate in veterinary critical care: pathophysiology and management. , 2007, Journal of the American Animal Hospital Association.

[12]  Roumiana Tsenkova,et al.  Essentials of Aquaphotomics and Its Chemometrics Approaches , 2018, Front. Chem..

[13]  Roumiana Tsenkova,et al.  Aquaphotomics: Dynamic Spectroscopy of Aqueous and Biological Systems Describes Peculiarities of Water , 2009 .

[14]  N Baishya,et al.  Near Infrared Spectrometric Investigations on the behaviour of Lactate , 2019, 2019 41st Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC).

[15]  L. Lands,et al.  In vivo lactate measurement in human tissue by near-infrared diffuse reflectance spectroscopy , 2004 .

[16]  Michael J. McShane,et al.  Near-Infrared Spectroscopy for Determination of Glucose, Lactate, and Ammonia in Cell Culture Media , 1998 .

[17]  Céline Occelli,et al.  Blood lactate measurement within the emergency department: A two‐year retrospective analysis , 2019, The American journal of emergency medicine.

[18]  Valery V Tuchin,et al.  Simple multimodal optical technique for evaluation of free/bound water and dispersion of human liver tissue , 2017, Journal of biomedical optics.