Long Noncoding RNA LINC00173 Promotes NUTF2 Expression Through Sponging miR-765 and Facilitates Tumorigenesis in Glioma

Background Glioma is one of the leading causes of cancer-related deaths. This study aimed to investigate the function and mechanism of long noncoding RNA (lncRNA) LINC00173 in the regulation of glioma progression. Methods LINC00173 expression was measured using qRT-PCR. Survival rate was analyzed through Kaplan–Meier method. CCK8, colony formation and EdU assays were performed to measure cell proliferation while transwell was used to determine cell migration and invasion. Luciferase reporter assay was conducted to test RNA interaction. Results LINC00173 expression was elevated in glioma tissues and cells. LINC00173 high expression predicted poor prognosis. Loss of LINC00173 inhibited proliferation, migration and invasion. LINC00173 interacted with miR-765 to enhance NUTF2 expression. MiR-765 expression was negatively correlated with LINC00173 and NUTF2 in glioma tissues. NUTF2 level was increased in glioma tissues. NUTF2 overexpression rescued the potential of proliferation, migration and invasion in LINC00173-silenced cells. Conclusion Our research demonstrated that LINC00173 promotes glioma progression through targeting miR-765/NUTF2 axis.

[1]  Huiping Wang,et al.  Chlamydial-Secreted Protease Chlamydia High Temperature Requirement Protein A (cHtrA) Degrades Human Cathelicidin LL-37 and Suppresses Its Anti-Chlamydial Activity , 2020, Medical science monitor : international medical journal of experimental and clinical research.

[2]  Chao Yang,et al.  Long non-coding RNA NCK1-AS1 promotes the tumorigenesis of glioma through sponging microRNA-138-2-3p and activating the TRIM24/Wnt/β-catenin axis , 2020, Journal of Experimental & Clinical Cancer Research.

[3]  Mei-Yi Tao,et al.  LncRNA CCAT2 promotes angiogenesis in glioma through activation of VEGFA signalling by sponging miR-424 , 2020, Molecular and Cellular Biochemistry.

[4]  Dong-hao Wu,et al.  MicroRNA-126 Inhibit Viability of Colorectal Cancer Cell by Repressing mTOR Induced Apoptosis and Autophagy , 2020, OncoTargets and therapy.

[5]  Bin Sun,et al.  Long noncoding RNA PVT1 contributes to vascular endothelial cell proliferation via inhibition of miR-190a-5p in diagnostic biomarker evaluation of chronic heart failure , 2020, Experimental and therapeutic medicine.

[6]  Yan Shi,et al.  Down-regulation of lncRNA LINC00152 Suppresses Gastric Cancer Cell Migration and Invasion Through Inhibition of the ERK/MAPK Signaling Pathway , 2020, OncoTargets and Therapy.

[7]  L. Zhuang,et al.  Long non‐coding RNA SNHG6 promotes the growth and invasion of non‐small cell lung cancer by downregulating miR‐101‐3p , 2020, Thoracic cancer.

[8]  Hongyi Liu,et al.  Long Noncoding RNA LINC00467 Promotes Glioma Progression through Inhibiting P53 Expression via Binding to DNMT1 , 2020, Journal of Cancer.

[9]  Jing Yuan,et al.  LncRNA LINC00173 enhances triple-negative breast cancer progression by suppressing miR-490-3p expression. , 2020, Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie.

[10]  Z. Kong,et al.  Progress and Prospects of Recurrent Glioma: A Recent Scientometric Analysis of the Web of Science in 2019 , 2020 .

[11]  Xiaobo Wang,et al.  PIK3C3 Acts as a Tumor Suppressor in Esophageal Squamous Cell Carcinoma and Was Regulated by MiR-340-5p , 2020, Medical science monitor : international medical journal of experimental and clinical research.

[12]  Yang Xiao,et al.  Regulating of cell cycle progression by the lncRNA CDKN2B-AS1/miR-324-5p/ROCK1 axis in laryngeal squamous cell cancer , 2020, The International journal of biological markers.

[13]  Qinghua Wang,et al.  Long non-coding RNA TTN-AS1 promotes breast cancer cell migration and invasion via sponging miR-140-5p , 2019, Oncology letters.

[14]  Xia Zeng,et al.  Long non‑coding RNA LINC00312 regulates breast cancer progression through the miR‑9/CDH1 axis. , 2019, Molecular medicine reports.

[15]  Yanjiao Li,et al.  LINC00703 Acts as a Tumor Suppressor via Regulating miR-181a/KLF6 Axis in Gastric Cancer , 2019, Journal of gastric cancer.

[16]  Xiaomei Li,et al.  Oncogenic microRNA‐765 promotes the growth and metastasis of breast carcinoma by directly targeting ING4 , 2019, Journal of cellular biochemistry.

[17]  Linlang Guo,et al.  Linc00173 promotes chemoresistance and progression of small cell lung cancer by sponging miR-218 to regulate Etk expression , 2019, Oncogene.

[18]  Qian Yang,et al.  Diminished LINC00173 expression induced miR-182-5p accumulation promotes cell proliferation, migration and apoptosis inhibition via AGER/NF-κB pathway in non-small-cell lung cancer. , 2019, American journal of translational research.

[19]  K. Gao,et al.  MicroRNA-765 targets MTUS1 to promote the progression of osteosarcoma via mediating ERK/EMT pathway. , 2019, European review for medical and pharmacological sciences.

[20]  Guoan Chen,et al.  MicroRNA-765 is pregulated in multiple myeloma and serves an oncogenic role by directly targeting SOX6. , 2019, Experimental and therapeutic medicine.

[21]  J. Mao,et al.  Long Noncoding RNA ASB16-AS1 Promotes Proliferation, Migration, and Invasion in Glioma Cells , 2019, BioMed research international.

[22]  Yang Niu,et al.  LINC00994 promoted invasion and proliferation of gastric cancer cell via regulating miR-765-3p. , 2019, American journal of translational research.

[23]  J. Barnholtz-Sloan,et al.  CBTRUS Statistical Report: Primary Brain and Other Central Nervous System Tumors Diagnosed in the United States in 2012-2016. , 2019, Neuro-oncology.

[24]  Ying Chen,et al.  Long non-coding RNA ROR promotes radioresistance in hepatocelluar carcinoma cells by acting as a ceRNA for microRNA-145 to regulate RAD18 expression. , 2018, Archives of biochemistry and biophysics.

[25]  Sen Yang,et al.  LINC00511 interacts with miR‐765 and modulates tongue squamous cell carcinoma progression by targeting LAMC2 , 2018, Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology.

[26]  Zhongxue Wu,et al.  Neuroprotective effects of miR-27a against traumatic brain injury via suppressing FoxO3a-mediated neuronal autophagy. , 2017, Biochemical and biophysical research communications.

[27]  J. Zhao,et al.  Expression and Functional Role of Reprogramming-Related Long Noncoding RNA (lincRNA-ROR) in Glioma , 2015, Journal of Molecular Neuroscience.