Optical Tools for Single-Cell Manipulation and Analysis

Experiments on individual cells require a range of extremely precise tools to permit their selection, manipulation, stimulation and analysis. This is further complicated by the cells’ sensitivity to their environment, meaning that such tools must also be very gentle (or at least very localised) to minimise the generation of artefacts. Optical tools provide ideal performance in a number of such roles, exhibiting high spatial and temporal selectivity while causing minimal non-specific effects. This chapter focuses upon the optical tools that have been developed for these purposes, ranging from optical trapping systems which provide a contact-free technique for the manipulation of micron-scale objects, through to a selection of the different optically mediated cell membrane disruption methods available for lysis and/or delivery of material.

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