Involvement of hydrogenases in the formation of highly catalytic Pd(0) nanoparticles by bioreduction of Pd(II) using Escherichia coli mutant strains.

Escherichia coli produces at least three [NiFe] hydrogenases (Hyd-1, Hyd-2 and Hyd-3). Hyd-1 and Hyd-2 are membrane-bound respiratory isoenzymes with their catalytic subunits exposed to the periplasmic side of the membrane. Hyd-3 is part of the cytoplasmically oriented formate hydrogenlyase complex. In this work the involvement of each of these hydrogenases in Pd(II) reduction under acidic (pH 2.4) conditions was studied. While all three hydrogenases could contribute to Pd(II) reduction, the presence of either periplasmic hydrogenase (Hyd-1 or Hyd-2) was required to observe Pd(II) reduction rates comparable to the parent strain. An E. coli mutant strain genetically deprived of all hydrogenase activity showed negligible Pd(II) reduction. Electron microscopy suggested that the location of the resulting Pd(0) deposits was as expected from the subcellular localization of the particular hydrogenase involved in the reduction process. Membrane separation experiments established that Pd(II) reductase activity is membrane-bound and that hydrogenases are required to initiate Pd(II) reduction. The catalytic activity of the resulting Pd(0) nanoparticles in the reduction of Cr(VI) to Cr(III) varied according to the E. coli mutant strain used for the initial bioreduction of Pd(II). Optimum Cr(VI) reduction, comparable to that observed with a commercial Pd catalyst, was observed when the bio-Pd(0) catalytic particles were prepared from a strain containing an active Hyd-1. The results are discussed in the context of economic production of novel nanometallic catalysts.

[1]  I. Mikheenko,et al.  Bioaccumulation of Palladium by Desulfovibrio fructosivorans Wild-Type and Hydrogenase-Deficient Strains , 2008, Applied and Environmental Microbiology.

[2]  K. Deplanche,et al.  Biorecovery of gold by Escherichia coli and Desulfovibrio desulfuricans , 2008, Biotechnology and bioengineering.

[3]  F. Sargent,et al.  Dissecting the roles of Escherichia coli hydrogenases in biohydrogen production. , 2008, FEMS microbiology letters.

[4]  J. Wood,et al.  Novel supported Pd hydrogenation bionanocatalyst for hybrid homogeneous/heterogeneous catalysis , 2007 .

[5]  Mark D. Redwood,et al.  Bio-hydrogen and biomass supported palladium catalyst for energy production and waste minimisation. Ph.D. Thesis. University of Birmingham. , 2007 .

[6]  K. Deplanche,et al.  Biorecovery of Gold from Jewellery Wastes by Escherichia Coli and Biomanufacture of Active Au-Nanomaterial , 2007 .

[7]  H. M. Thompson,et al.  The decolorization of sugar liquor by bone charcoal , 2007 .

[8]  Stuart Harrad,et al.  Dehalogenation of polychlorinated biphenyls and polybrominated diphenyl ethers using a hybrid bioinorganic catalyst. , 2007, Journal of environmental monitoring : JEM.

[9]  D Barrie Johnson,et al.  The microbiology of biomining: development and optimization of mineral-oxidizing microbial consortia. , 2007, Microbiology.

[10]  I. Mikheenko,et al.  From bio-mineralisation to fuel cells: biomanufacture of Pt and Pd nanocrystals for fuel cell electrode catalyst , 2007, Biotechnology Letters.

[11]  Lynne E. Macaskie,et al.  Palladium and gold removal and recovery from precious metal solutions and electronic scrap leachates by Desulfovibrio desulfuricans , 2006, Biotechnology Letters.

[12]  S. Meroueh,et al.  Three-dimensional structure of the bacterial cell wall peptidoglycan. , 2006, Proceedings of the National Academy of Sciences of the United States of America.

[13]  L. Macaskie,et al.  Biorecovered precious metals from industrial wastes: single-step conversion of a mixed metal liquid waste to a bioinorganic catalyst with environmental application. , 2006, Environmental science & technology.

[14]  Toshiyuki Nomura,et al.  Intracellular recovery of gold by microbial reduction of AuCl4− ions using the anaerobic bacterium Shewanella algae , 2006 .

[15]  W. Verstraete,et al.  Bioreductive deposition of palladium (0) nanoparticles on Shewanella oneidensis with catalytic activity towards reductive dechlorination of polychlorinated biphenyls. , 2005, Environmental microbiology.

[16]  I. Mikheenko,et al.  Applications of bacterial hydrogenases in waste decontamination, manufacture of novel bionanocatalysts and in sustainable energy. , 2005, Biochemical Society transactions.

[17]  L. Macaskie,et al.  Continuous removal of Cr(VI) from aqueous solution catalysed by palladised biomass of Desulfovibrio vulgaris , 2004, Biotechnology Letters.

[18]  M. Faramarzi,et al.  Metal solubilization from metal-containing solid materials by cyanogenic Chromobacterium violaceum. , 2004, Journal of biotechnology.

[19]  I. Mikheenko,et al.  Sulphate-reducing bacteria, palladium and the reductive dehalogenation of chlorinated aromatic compounds , 2003, Biodegradation.

[20]  L. Macaskie,et al.  Biosorption of palladium and platinum by sulfate‐reducing bacteria , 2004 .

[21]  G. Sawers.  The hydrogenases and formate dehydrogenases ofEscherichia coli , 2004, Antonie van Leeuwenhoek.

[22]  B. Guigliarelli,et al.  Hydrogenases in sulfate-reducing bacteria function as chromium reductase , 2003, Applied Microbiology and Biotechnology.

[23]  J. Lloyd.  Microbial reduction of metals and radionuclides. , 2003, FEMS microbiology reviews.

[24]  Yung-Sheng Chang,et al.  Regulation of the Hydrogenase-4 Operon of Escherichia coli by the σ54-Dependent Transcriptional Activators FhlA and HyfR , 2002, Journal of bacteriology.

[25]  I. R. Harris,et al.  Bioreduction and biocrystallization of palladium by Desulfovibrio desulfuricans NCIMB 8307 , 2002, Biotechnology and bioengineering.

[26]  T. Palmer,et al.  How bacteria get energy from hydrogen: a genetic analysis of periplasmic hydrogen oxidation in Escherichia coli , 2002 .

[27]  J. Lloyd,et al.  Effect of complexing agents on reduction of Cr(VI) by Desulfovibrio vulgaris ATCC 29579. , 2002, Biotechnology and bioengineering.

[28]  I. R. Harris,et al.  Bioaccumulation of palladium by Desulfovibrio desulfuricans , 2002 .

[29]  G. De Luca,et al.  Reduction of Technetium(VII) byDesulfovibrio fructosovorans Is Mediated by the Nickel-Iron Hydrogenase , 2001, Applied and Environmental Microbiology.

[30]  Kelly P. Nevin,et al.  Reductive Precipitation of Gold by Dissimilatory Fe(III)-Reducing Bacteria andArchaea , 2001, Applied and Environmental Microbiology.

[31]  J. Lloyd,et al.  Metal reduction by sulphate-reducing bacteria: Physiological diversity and metal specificity , 2001 .

[32]  B. Berks,et al.  Sec-independent Protein Translocation in Escherichia coli , 1999, The Journal of Biological Chemistry.

[33]  J. Lloyd,et al.  Reduction of Technetium by Desulfovibrio desulfuricans: Biocatalyst Characterization and Use in a Flowthrough Bioreactor , 1999, Applied and Environmental Microbiology.

[34]  Lynne E. Macaskie,et al.  Enzymatic Recovery of Elemental Palladium by Using Sulfate-Reducing Bacteria , 1998, Applied and Environmental Microbiology.

[35]  J. Lloyd,et al.  Tc(VII) reduction and accumulation by immobilized cells of Escherichia coli. , 1997, Biotechnology and bioengineering.

[36]  J. Lloyd,et al.  Reduction and removal of heptavalent technetium from solution by Escherichia coli , 1997, Journal of bacteriology.

[37]  L. Yanke,et al.  Hydrogenase I of Clostridium pasteurianum functions as a novel selenite reductase. , 1995, Anaerobe.

[38]  D. Lovley,et al.  Dissimilatory metal reduction. , 1993, Annual review of microbiology.

[39]  D. Lovley,et al.  Reduction of uranium by Desulfovibrio desulfuricans , 1992, Applied and environmental microbiology.

[40]  D. Lide.  Handbook of Chemistry and Physics , 1992 .

[41]  B. Washburn,et al.  New method for generating deletions and gene replacements in Escherichia coli , 1989, Journal of bacteriology.

[42]  L. Tranvik,et al.  Microbial degradation of xenobiotic, aromatic pollutants in humic water , 1988, Applied and environmental microbiology.

[43]  R. Sawers,et al.  Differential expression of hydrogenase isoenzymes in Escherichia coli K-12: evidence for a third isoenzyme , 1985, Journal of bacteriology.

[44]  D. Boxer,et al.  Nickel-containing hydrogenase isoenzymes from anaerobically grown Escherichia coli K-12 , 1985, Journal of bacteriology.

[45]  S. Cohen,et al.  Lactose genes fused to exogenous promoters in one step using a Mu-lac bacteriophage: in vivo probe for transcriptional control sequences. , 1979, Proceedings of the National Academy of Sciences of the United States of America.

[46]  R. C. Weast.  CRC Handbook of Chemistry and Physics , 1973 .