Mixing enhancement by biologically inspired convection in a micro-chamber using alternating current galvanotactic control of the Tetrahymena pyriformis

Recently, there has been increasing interest in the swimming behavior of microorganisms and biologically inspired micro-robots. In this study, we investigated biologically induced convection flow with living microorganism using galvanotaxis. We fabricated and evaluated our micro-mixer with motile cells. For the cell based active micro-mixers, two miscible fluids were used to measure the mixing index. Under alternating current (AC) electric fields with varying frequency, a group of motile Tetrahymena pyriformis cells generated reciprocal motion with circulating flows around their pathline, enhancing the mixing ratio.

[1]  Elinore M Mercer,et al.  Microfluidic sorting of mammalian cells by optical force switching , 2005, Nature Biotechnology.

[2]  D A Weitz,et al.  Surface acoustic wave actuated cell sorting (SAWACS). , 2010, Lab on a chip.

[3]  M. J. Kim,et al.  Artificial magnetotactic motion control of Tetrahymena pyriformis using ferromagnetic nanoparticles: A tool for fabrication of microbiorobots , 2010 .

[4]  G. Whitesides,et al.  Microfluidic devices fabricated in Poly(dimethylsiloxane) for biological studies , 2003, Electrophoresis.

[5]  Dick Broer,et al.  Artificial cilia for active micro-fluidic mixing. , 2008, Lab on a chip.

[6]  Anthony W Smith,et al.  Biofilms and antibiotic therapy: is there a role for combating bacterial resistance by the use of novel drug delivery systems? , 2005, Advanced drug delivery reviews.

[7]  Masatoshi Ishikawa,et al.  A physical model for galvanotaxis of Paramecium cell. , 2006, Journal of theoretical biology.

[8]  J. Adler Chemotaxis in Bacteria , 1966, Science.

[9]  Min Jun Kim,et al.  Galvanotactic and phototactic control of Tetrahymena pyriformis as a microfluidic workhorse , 2009 .

[10]  Patrick Patrick Anderson,et al.  Active micromixer based on artificial cilia , 2007 .

[11]  L. Jang,et al.  Fabrication and Characterization of PZT Thick Films for Sensing and Actuation , 2007, Sensors (Basel, Switzerland).

[12]  M. J. Kim,et al.  Control of microfabricated structures powered by flagellated bacteria using phototaxis , 2007 .

[13]  Jihoon Kim,et al.  Quantitative measurement of dynamic flow induced by Tetrahymena pyriformis (T. pyriformis) using micro-particle image velocimetry , 2011, J. Vis..

[14]  V. Hessel,et al.  Micromixers—a review on passive and active mixing principles , 2005 .

[15]  J. Blake,et al.  A spherical envelope approach to ciliary propulsion , 1971, Journal of Fluid Mechanics.

[16]  G. A. Kerkut,et al.  Galvanotaxic response of tetrahymena vorax , 1981 .