Distribution of endocrine disruptors in the Mondego River estuary, Portugal

Recent studies in the Mondego River estuary demonstrated signs of pollution in the area, but the nature of the contamination remains unexplored. Because there seems to be any studies in that zone checking for the simultaneous presence of xenoestrogenic endocrine disrupting compounds, either of animal (estradiol and estrone), vegetal (daidzein, genistein and biochanin A), pharmaceutical (17α-ethynylestradiol) or industrial (bisphenol A, 4-octylphenol, 4-nonylphenol) origins, the main objective of this study was to investigate their presence, in every year season and at eight points along the estuarine gradient. For this propose, water samples (2 L) were collected in high and low tides, preconcentrated in the Oasis HLB cartridges and cleaned in silica cartridges before their analysis by HPLC-DAD and GC-MS. The current data showed the absence of contamination by animal or pharmaceutical estrogens and by alkylphenols, but demonstrated the presence of high levels of phytoestrogens (up to 1.1 μg/L) and of bisphenol A (up to 880.0 ng/L). Because these two chemicals existed in high environmental concentrations in the most upstream sampling station, and taking in account that these pollutants may additively contribute to endocrine disruption, namely on fish, it was concluded that at least this particularly area of the Mondego estuary deserves continuous monitoring programmes.

[1]  D. Barceló,et al.  Survey of organotin compounds in rivers and coastal environments in Portugal 1999-2000. , 2005, Environmental pollution.

[2]  A. D. Vethaak,et al.  An integrated assessment of estrogenic contamination and biological effects in the aquatic environment of The Netherlands. , 2005, Chemosphere.

[3]  B. Chang,et al.  Anaerobic degradation of nonylphenol in sludge. , 2005, Chemosphere.

[4]  C. Chichester,et al.  Review of evidence: are endocrine-disrupting chemicals in the aquatic environment impacting fish populations? , 2005, The Science of the total environment.

[5]  J. Vílchez,et al.  Sensitive gas chromatographic-mass spectrometric method for the determination of phthalate esters, alkylphenols, bisphenol A and their chlorinated derivatives in wastewater samples. , 2006, Journal of chromatography. A.

[6]  Jeong-Hun Kang,et al.  Bisphenol A degradation in seawater is different from that in river water. , 2005, Chemosphere.

[7]  D. Vagiona,et al.  Diversity of Salmonella spp. and fungi in northern Greek rivers and their correlation to fecal pollution indicators. , 2005, Environmental research.

[8]  Wolfgang Ruck,et al.  Liquid chromatography-tandem mass spectrometry analysis of estrogenic compounds in coastal surface water of the Baltic Sea. , 2005, Journal of chromatography. A.

[9]  M. Pardal,et al.  Seasonal Variation of Surface Sediments Composition in Mondego River Estuary , 2005, Journal of environmental science and health. Part A, Toxic/hazardous substances & environmental engineering.

[10]  W. Giger,et al.  Aerobic transformation of short-chain alkylphenol polyethoxylates by mixed bacterial cultures , 1994 .

[11]  John P. Sumpter,et al.  Widespread Sexual Disruption in Wild Fish , 1998 .

[12]  J. Marques,et al.  Bacterioplankton dynamics in the Mondego estuary (Portugal) , 2003 .

[13]  C. Metcalfe,et al.  Effects of the isoflavones genistein and equol on the gonadal development of Japanese medaka Oryzias latipes. , 2003, Environmental health perspectives.

[14]  Charles A. Staples,et al.  A Weight of Evidence Approach to the Aquatic Hazard Assessment of Bisphenoi A , 2002 .

[15]  S. Kim,et al.  Determination of phytoestrogens in traditional medicinal herbs using gas chromatography--mass spectrometry. , 2004, The Journal of nutritional biochemistry.

[16]  R. Cela,et al.  Determination of natural and synthetic estrogens in water by gas chromatography with mass spectrometric detection. , 2004, Journal of chromatography. A.

[17]  Gerhard Nahler,et al.  International Conference on Harmonisation (ICH) , 2009 .

[18]  Marques,et al.  Impact of macroalgal blooms and wader predation on intertidal macroinvertebrates: experimental evidence from the Mondego estuary (Portugal). , 2000, Journal of experimental marine biology and ecology.

[19]  D. Raffaelli,et al.  Macroinvertebrate response to different species of macroalgal mats and the role of disturbance history. , 2004 .

[20]  M. Pardal,et al.  Population dynamics of Cyathura carinata (Isopoda) in a eutrophic temperate estuary , 2004 .

[21]  M. Angove,et al.  Optimization of silylation using N-methyl-N-(trimethylsilyl)-trifluoroacetamide, N,O-bis-(trimethylsilyl)-trifluoroacetamide and N-(tert-butyldimethylsilyl)-N-methyltrifluoroacetamide for the determination of the estrogens estrone and 17alpha-ethinylestradiol by gas chromatography-mass spectrometry. , 2006, Journal of chromatography. A.

[22]  T. Romeo,et al.  Evaluation of ecotoxicological effects of endocrine disrupters during a four-year survey of the Mediterranean population of swordfish (Xiphias gladius). , 2004, Marine environmental research.

[23]  João Carlos Marques,et al.  Management of a shallow temperate estuary to control eutrophication: The effect of hydrodynamics on the system’s nutrient loading , 2005 .

[24]  S. Runswick,et al.  Daidzein and genistein content of cereals , 2002, European Journal of Clinical Nutrition.

[25]  E. Abad,et al.  Assessment of toxicological status of a SW Mediterranean segment population of striped dolphin (Stenella coeruleoalba) using skin biopsy. , 2004, Marine environmental research.

[26]  M. Pardal,et al.  Biology, population dynamics and secondary production of the green crab Carcinus maenas (L.) in a temperate estuary , 2005 .

[27]  J. Sumpter,et al.  Altered Sexual Maturation and Gamete Production in Wild Roach (Rutilus rutilus) Living in Rivers That Receive Treated Sewage Effluents1 , 2002, Biology of reproduction.

[28]  C. Ribeiro,et al.  Development and Validation of a HPLC‐DAD Method for Determination of Several Endocrine Disrupting Compounds in Estuarine Water , 2007 .

[29]  J. Sumpter,et al.  Reproductive effects of long-term exposure to Bisphenol A in the fathead minnow (Pimephales promelas). , 2001, Environmental science & technology.

[30]  N. Booth,et al.  Seasonal variation of red clover (Trifolium pratense L., Fabaceae) isoflavones and estrogenic activity. , 2006, Journal of agricultural and food chemistry.