Normal‐Phase and Polar Organic Solvents Chromatography

This chapter starts with a brief overview of chromatography and focuses on liquid chromatography, particularly NPLC and POSC. Retention mechanisms both for polarity-based separations seen in NPLC and POSC and for charge and size-based separations are explained. The second part of the chapter focuses on different stationary and mobile phases that can be combined together to give a chromatographic system with an acceptable separation and required selectivity. Factors that should be kept in mind when switching from one stationary or mobile phase to another are highlighted and examples that facilitate understanding are used. Keywords: normal-phase liquid chromatography; polar organic solvents chromatography; retention mechanisms; stationary phases; mobile phases

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