WebSET is an Internet based educational tool that can be used on any standard personal computer. It has been developed by a European collaboration and integrates high quality courseware, self-assessment exercises, video clips and VR simulation. This pilot study was carried out to validate the lumbar puncture (LP) module in training. Subjects who had never performed an LP participated in the study. The subjects were randomized into 2 groups: 1) underwent training and 2) control group. Both groups performed a pre-test and post-test LP on a synthetic mannequin. There was a significant improvement in the training group in terms of performance assessment as compared to the control group. This study showed that there was a significant improvement in the performance of the procedure and in procedural knowledge after training with WebSET.