Recent Advances in Fluorescent Labeling Techniques for Fluorescence Microscopy

Tremendous progress in recent computer-controlled systems for fluorescence and laser-confocal microscopy has provided us with powerful tools to visualize and analyze molecular events in the cells. Various fluorescent staining and labeling techniques have also been developed to be used with these powerful instruments. Fluorescent proteins such as green fluorescent protein (GFP) allow us to directly label particular proteins of interest in living cells. This technique has been extended over a large area of cell biology, and a variety of fluorescent protein-derived techniques have been developed to visualize the functions and conditions of the molecules within living cells. In this review, we summarize the techniques for fluorescent staining and labeling for recent fluorescence microscopy.

[1]  K. Takata,et al.  Transport of glucose across the blood-tissue barriers. , 1997, International review of cytology.

[2]  K. Takata,et al.  Nuclear Staining for Laser Confocal Microscopy , 1997 .

[3]  S. Lukyanov,et al.  GFP‐like chromoproteins as a source of far‐red fluorescent proteins , 2001, FEBS letters.

[4]  P. Agre.  Aquaporin Water Channels in Kidney , 2000 .

[5]  S. Jakobs,et al.  Short tetracysteine tags to beta-tubulin demonstrate the significance of small labels for live cell imaging. , 2004, Molecular biology of the cell.

[6]  K. Takata,et al.  Apical Localization of Sodium-Dependent Glucose Transporter SGLT1 is Maintained by Cholesterol and Microtubules , 2006, Acta histochemica et cytochemica.

[7]  Takeharu Nagai,et al.  Shift anticipated in DNA microarray market , 2002, Nature Biotechnology.

[8]  K. Takata,et al.  Aquaporins: water channel proteins of the cell membrane. , 2004, Progress in histochemistry and cytochemistry.

[9]  K. Takata,et al.  Water Channel Aquaporin 1 (AQP1) Is Present in the Perineurium and Perichondrium , 2005 .

[10]  R. Tsien,et al.  A monomeric red fluorescent protein , 2002, Proceedings of the National Academy of Sciences of the United States of America.

[11]  S. Ohmori,et al.  Spatiotemporal Analysis of the Molecular Interaction between PICK1 and PKC , 2006, Acta histochemica et cytochemica.

[12]  David M. Miller,et al.  Chapter 16 Immunofluorescence Microscopy , 1995 .

[13]  K. Takata,et al.  Localization of Golgi 58K protein (formiminotransferase cyclodeaminase) to the centrosome , 2006, Histochemistry and Cell Biology.

[14]  M. Matsuda,et al.  Visualizing the Signal Transduction Pathways in Living Cells with GFP-Based FRET Probes , 2004 .

[15]  A. Miyawaki.  Visualization of the spatial and temporal dynamics of intracellular signaling. , 2003, Developmental cell.

[16]  A. Miyawaki,et al.  Spatio-temporal images of growth-factor-induced activation of Ras and Rap1 , 2001, Nature.

[17]  A. Nakano,et al.  Live imaging of yeast Golgi cisternal maturation , 2006, Nature.

[18]  R Y Tsien,et al.  Specific covalent labeling of recombinant protein molecules inside live cells. , 1998, Science.

[19]  J. Lippincott-Schwartz,et al.  Maintenance of Golgi structure and function depends on the integrity of ER export , 2001, The Journal of cell biology.

[20]  S. Carrasco,et al.  Diacylglycerol-dependent binding recruits PKCtheta and RasGRP1 C1 domains to specific subcellular localizations in living T lymphocytes. , 2004, Molecular biology of the cell.

[21]  R. Tsien,et al.  The Fluorescent Toolbox for Assessing Protein Location and Function , 2006, Science.

[22]  T. Matsuzaki,et al.  An Anti-Peptide Antibody that Recognized Unexpected Protein -A Case Report , 2000 .

[23]  R. Bartenschlager,et al.  Targeting of Hepatitis C Virus Core Protein to Mitochondria through a Novel C-Terminal Localization Motif , 2004, Journal of Virology.

[24]  D. Sakai,et al.  Cooperative action of Sox9, Snail2 and PKA signaling in early neural crest development , 2006, Development.

[25]  A. Joshi,et al.  Cloning, Expression, Characterization, and Interaction of Two Components of a Human Mitochondrial Fatty Acid Synthase , 2003, Journal of Biological Chemistry.

[26]  R L Shoemaker,et al.  Digital microscopy. , 1984, Monographs in clinical cytology.

[27]  Thomas J Deerinck,et al.  Multicolor and Electron Microscopic Imaging of Connexin Trafficking , 2002, Science.

[28]  J. Kere,et al.  SLC26A7: a basolateral Cl-/HCO3- exchanger specific to intercalated cells of the outer medullary collecting duct. , 2004, American journal of physiology. Renal physiology.

[29]  Takeshi Suzuki,et al.  Fluorescence Counter-Staining of Cell Nuclear DNA for Multi-Color Laser Confocal Microscopy , 1998 .

[30]  Hideo Tanaka,et al.  Spatiotemporal Visualization of Intracellular Ca2+ in Living Heart Muscle Cells Viewed by Confocal Laser Scanning Microscopy , 2003 .

[31]  P. Várnai,et al.  Visualization and manipulation of phosphoinositide dynamics in live cells using engineered protein domains , 2007, Pflügers Archiv - European Journal of Physiology.

[32]  M. Tamura,et al.  Microenvironment Analysis in Squid Axons Using Fluorescence Correlation Spectroscopy and Laser Scanning Microscopy , 2002 .

[33]  Alexander Kamb,et al.  Stable expression of Anthozoa fluorescent proteins in mammalian cells. , 2002, Cytometry.

[34]  D. Shakes,et al.  Immunofluorescence microscopy. , 1995, Methods in cell biology.

[35]  Nathan C Shaner,et al.  A guide to choosing fluorescent proteins , 2005, Nature Methods.

[36]  K. Takata,et al.  DNA Staining for Fluorescence and Laser Confocal Microscopy , 1997, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.