Selective uricase biosensor based on polyaniline synthesized in ionic liquid

Abstract The selective uricase biosensor prepared with electroactive intrinsic polyaniline (PANI) at high pH is firstly reported in this paper. Effects of electroactive interferents, such as acetaminophen, glutathione, l -cysteine and ascorbic acid on the current response of PANI–uricase biosensor were examined in the presence of their physiological normal levels with uric acid (HUr) concentration at 0.5 mmol dm−3. These well-known interferents have been shown to have no measurable effect on the biosensor's current response. In the range from 0.20 to 0.50 V, the current response increased with increasing potential. In the wide pH range from 2 to 12, current response increased with increasing pH, and the optimum pH was not appearing. We suggested the reason why the optimum pH of the PANI–uricase biosensor does not appear. The relationship between current response and uric acid concentration is good linear in the range from 1.0 × 10−3 to 1.0 mmol dm−3. The Michaelis–Menten constant K ′ m is 7.83 mmol dm−3 and the maximum current response imax is 58.07 μA. The current response of this biosensor increased with increasing temperature and Ea is 40.18 kJ mol−1 in the B–R buffer. The biosensor was characterized with FT-IR, UV–vis spectra and AC impedance.

[1]  Asha Chaubey,et al.  Application of conducting polymers to biosensors. , 2002, Biosensors & bioelectronics.

[2]  B. D. Malhotra,et al.  Application of polyaniline as enzyme based biosensor , 2005 .

[3]  G. S. Wilson,et al.  Rotating ring-disk enzyme electrode for surface catalysis studies. , 1976, Analytical chemistry.

[4]  T. Fuchigami,et al.  Electrooxidative polymerization of aromatic compounds in 1-ethyl-3-methylimidazolium trifluoromethanesulfonate room-temperature ionic liquid , 2003 .

[5]  H. Britton,et al.  LXI.—The use of the antimony–antimonous oxide electrode in the determination of the concentration of hydrogen ions and in potenliometric titrations. The Prideaux–Ward universal buffer mixture , 1931 .

[6]  J. A. Laszlo,et al.  Bioelectrocatalysis in ionic liquids. Examining specific cation and anion effects on electrode-immobilized cytochrome c. , 2006, Bioelectrochemistry.

[7]  G. Evtugyn,et al.  Polyaniline-modified cholinesterase sensor for pesticide determination. , 2002, Bioelectrochemistry.

[8]  Yongfang Li,et al.  Effect of nonionic surfactant additives on the electropolymerization of pyrrole in aqueous solutions , 2000 .

[9]  Mu Shaolin.  Bioelectrochemical response of the polyaniline galactose oxidase electrode , 1994 .

[10]  Suman,et al.  Galactose sensor based on galactose oxidase immobilized in polyvinyl formal , 2006 .

[11]  G. Wallace,et al.  Conducting polymer nanoparticles synthesized in an ionic liquid by chemical polymerisation , 2006 .

[12]  H. Ju,et al.  Preparation of ormosil and its applications in the immobilizing biomolecules , 2006 .

[13]  Bansi D. Malhotra,et al.  Cholesterol biosensor based on cholesterol esterase, cholesterol oxidase and peroxidase immobilized onto conducting polyaniline films , 2006 .

[14]  Rongguan Lv,et al.  Effect of ethanol on properties of electrochemically synthesized polyaniline , 2004 .

[15]  J. Kan,et al.  Polyaniline-uricase biosensor prepared with template process. , 2004, Biosensors & bioelectronics.

[16]  Yanrong Li,et al.  Fabrication of self-assembled polyaniline films by doping-induced deposition , 2000 .

[17]  E. Min,et al.  Ionic liquids: applications in catalysis , 2002 .

[18]  D. Bélanger,et al.  Electrochemistry of the polypyrrole glucose oxidase electrode , 1989 .

[19]  Mu Shaolin.  The kinetics of activated uricase immobilized on a polypyrrole film , 1994 .

[20]  Mu Shaolin,et al.  BIOELECTROCHEMICAL RESPONSES OF THE POLYANILINE GLUCOSE OXIDASE ELECTRODE , 1991 .

[21]  Mu Shaolin,et al.  Bioelectrochemical responses of the polyaniline uricase electrode , 1992 .

[22]  Huaiguo Xue,et al.  Bioelectrochemical response of the polypyrrole xanthine oxidase electrode , 1995 .

[23]  R. P. Swatloski,et al.  Efficient, halide free synthesis of new, low cost ionic liquids: 1,3-dialkylimidazolium salts containing methyl- and ethyl-sulfate anions , 2002 .

[24]  G. Wallace,et al.  Electrochemical synthesis of polypyrrole in ionic liquids , 2004 .

[25]  L. C. Clark,et al.  ELECTRODE SYSTEMS FOR CONTINUOUS MONITORING IN CARDIOVASCULAR SURGERY , 1962 .

[26]  F. Kuralay,et al.  Potentiometric enzyme electrode for urea determination using immobilized urease in poly(vinylferrocenium) film , 2005 .

[27]  Huang,et al.  Polaron lattice in highly conducting polyaniline: Theoretical and optical studies. , 1987, Physical review letters.

[28]  J. Kan,et al.  Preparation and properties of an uricase biosensor based on copolymer of o-aminophenol-aniline , 2006 .

[29]  B. Saidani,et al.  Electropolymerization of polypyrrole, modified with germanium, on a passivated titanium electrode in aqueous nitrate solution: new results on catalytic reduction of protons and dissolved oxygen , 2001 .