Electro-Fermentation: How To Drive Fermentation Using Electrochemical Systems.

Electro-fermentation (EF) is a novel process that consists of electrochemically controlling microbial fermentative metabolism with electrodes. The electrodes can act as either electron sinks or sources that allow unbalanced fermentation. They can also modify the medium by changing the redox balance. Such electrochemical control exerts significant effects not only on microbial metabolism and cellular regulation but also on interspecies interactions and the selection of bacterial populations in mixed microbial cultures. In this paper we propose some basics and principles to better define the EF concept within the field of bioelectrochemistry. We also explore the up-to-date strategies to put EF into practice and propose hypothetical mechanisms that could explain the first EF results reported in the literature.

[1]  T. Wu,et al.  A review of sustainable hydrogen production using seed sludge via dark fermentation , 2014 .

[2]  Jens O Krömer,et al.  Electrifying white biotechnology: engineering and economic potential of electricity-driven bio-production. , 2015, ChemSusChem.

[3]  S. Venkata Mohan,et al.  Synergistic yield of dual energy forms through biocatalyzed electrofermentation of waste: Stoichiometric analysis of electron and carbon distribution , 2015 .

[4]  S. Freguia,et al.  Development of bioelectrocatalytic activity stimulates mixed-culture reduction of glycerol in a bioelectrochemical system , 2015, Microbial biotechnology.

[5]  Kenneth H. Nealson,et al.  Microbial metabolic networks in a complex electrogenic biofilm recovered from a stimulus-induced metatranscriptomics approach , 2015, Scientific Reports.

[6]  D. Lovley Reach out and touch someone: potential impact of DIET (direct interspecies energy transfer) on anaerobic biogeochemistry, bioremediation, and bioenergy , 2011 .

[7]  Say Chye Joachim Loo,et al.  Metabolite-enabled mutualistic interaction between Shewanella oneidensis and Escherichia coli in a co-culture using an electrode as electron acceptor , 2015, Scientific Reports.

[8]  Raffaello Cossu,et al.  Urban mining: Concepts, terminology, challenges. , 2015, Waste management.

[9]  Kelly P. Nevin,et al.  Interspecies Electron Transfer via Hydrogen and Formate Rather than Direct Electrical Connections in Cocultures of Pelobacter carbinolicus and Geobacter sulfurreducens , 2012, Applied and Environmental Microbiology.

[10]  Jeffrey A. Gralnick,et al.  Enabling Unbalanced Fermentations by Using Engineered Electrode-Interfaced Bacteria , 2010, mBio.

[11]  Korneel Rabaey,et al.  Carbon and electron fluxes during the electricity driven 1,3-propanediol biosynthesis from glycerol. , 2013, Environmental science & technology.

[12]  K. Chandrasekhar,et al.  Solid phase bio-electrofermentation of food waste to harvest value-added products associated with waste remediation. , 2015, Waste management.

[13]  H. Beyenal,et al.  Microscale gradients and their role in electron-transfer mechanisms in biofilms. , 2012, Biochemical Society transactions.

[14]  Kelly P. Nevin,et al.  Carbon cloth stimulates direct interspecies electron transfer in syntrophic co-cultures. , 2014, Bioresource technology.

[15]  Bruce E. Rittmann,et al.  Syntrophic interactions among anode respiring bacteria (ARB) and Non‐ARB in a biofilm anode: electron balances , 2009, Biotechnology and bioengineering.

[16]  W. Verstraete,et al.  Microbial fuel cells: novel biotechnology for energy generation. , 2005, Trends in biotechnology.

[17]  Willy Verstraete,et al.  100 years of microbial electricity production: three concepts for the future , 2012, Microbial biotechnology.

[18]  Zhen He,et al.  Microbial desalination cells as a versatile technology: Functions, optimization and prospective , 2015 .

[19]  Valeria Mapelli,et al.  Electrochemical startup increases 1,3-propanediol titers in mixed-culture glycerol fermentations , 2015 .

[20]  O. Choi,et al.  Butyrate production enhancement by Clostridium tyrobutyricum using electron mediators and a cathodic electron donor , 2012, Biotechnology and bioengineering.

[21]  Jiangning Song,et al.  Metabolic Changes in Klebsiella oxytoca in Response to Low Oxidoreduction Potential, as Revealed by Comparative Proteomic Profiling Integrated with Flux Balance Analysis , 2014, Applied and Environmental Microbiology.

[22]  F. Bai,et al.  Redox potential control and applications in microaerobic and anaerobic fermentations. , 2013, Biotechnology advances.

[23]  Sven Kerzenmacher,et al.  Unbalanced fermentation of glycerol in Escherichia coli via heterologous production of an electron transport chain and electrode interaction in microbial electrochemical cells. , 2015, Bioresource technology.

[24]  P. Parameswaran,et al.  Relieving the fermentation inhibition enables high electron recovery from landfill leachate in a microbial electrolysis cell , 2016 .

[25]  Byung Hong Kim,et al.  Electron flow shift inClostridiumacetobutylicum fermentation by electrochemically introduced reducing equivalent , 1988, Biotechnology Letters.

[26]  K. Rabaey,et al.  Microbial electrosynthesis — revisiting the electrical route for microbial production , 2010, Nature Reviews Microbiology.

[27]  Bruce E Logan,et al.  Microbial electrolysis cells for high yield hydrogen gas production from organic matter. , 2008, Environmental science & technology.

[28]  L. Pan,et al.  Effects of culture redox potential on succinic acid production by Corynebacterium crenatum under anaerobic conditions , 2012 .

[29]  Kelly P. Nevin,et al.  Electrobiocommodities: powering microbial production of fuels and commodity chemicals from carbon dioxide with electricity. , 2013, Current opinion in biotechnology.

[30]  Korneel Rabaey,et al.  Dynamics of Cathode-Associated Microbial Communities and Metabolite Profiles in a Glycerol-Fed Bioelectrochemical System , 2013, Applied and Environmental Microbiology.

[31]  Low-Potential Respirators Support Electricity Production in Microbial Fuel Cells , 2015, Microbial Ecology.

[32]  C. Du,et al.  Use of oxidoreduction potential as an indicator to regulate 1,3-propanediol fermentation by Klebsiella pneumoniae , 2005, Applied Microbiology and Biotechnology.

[33]  Gemma Reguera,et al.  Fermentation of glycerol into ethanol in a microbial electrolysis cell driven by a customized consortium. , 2014, Environmental science & technology.

[34]  Frauke Kracke,et al.  Identifying target processes for microbial electrosynthesis by elementary mode analysis , 2014, BMC Bioinformatics.

[35]  S Venkata Mohan,et al.  Closed circuitry operation influence on microbial electrofermentation: Proton/electron effluxes on electro-fuels productivity. , 2015, Bioresource technology.

[36]  Kelly P. Nevin,et al.  Electrical Conductivity in a Mixed-Species Biofilm , 2012, Applied and Environmental Microbiology.

[37]  Irini Angelidaki,et al.  Microbial electrolysis cells turning to be versatile technology: recent advances and future challenges. , 2014, Water research.

[38]  Kelly P. Nevin,et al.  Enhancing syntrophic metabolism in up-flow anaerobic sludge blanket reactors with conductive carbon materials. , 2015, Bioresource technology.

[39]  S. Venkata Mohan,et al.  Microbial fuel cell: Critical factors regulating bio-catalyzed electrochemical process and recent advancements , 2014 .

[40]  J. C. Thrash,et al.  Review: Direct and indirect electrical stimulation of microbial metabolism. , 2008, Environmental science & technology.

[41]  Wan Ramli Wan Daud,et al.  Biocathode in microbial electrolysis cell; Present status and future prospects , 2015 .

[42]  Damien J Batstone,et al.  Regulation mechanisms in mixed and pure culture microbial fermentation , 2014, Biotechnology and bioengineering.

[43]  Piet N.L. Lens,et al.  A review on dark fermentative biohydrogen production from organic biomass: Process parameters and use of by-products , 2015 .

[44]  Jeffrey Green,et al.  Bacterial redox sensors , 2004, Nature Reviews Microbiology.

[45]  Byoung-In Sang,et al.  Extracellular electron transfer from cathode to microbes: application for biofuel production , 2016, Biotechnology for Biofuels.

[46]  S. Venkata Mohan,et al.  Electro-fermentation of real-field acidogenic spent wash effluents for additional biohydrogen production with simultaneous treatment in a microbial electrolysis cell , 2015 .

[47]  Bruce E Logan,et al.  The electric picnic: synergistic requirements for exoelectrogenic microbial communities. , 2011, Current opinion in biotechnology.

[48]  Bernhard Schink,et al.  Batch and continuous production of propionic acid from whey permeate by Propionibacterium acidi-propionici in a three-electrode amperometric culture system , 1992, Applied Microbiology and Biotechnology.

[49]  Youngsoon Um,et al.  Electricity-driven metabolic shift through direct electron uptake by electroactive heterotroph Clostridium pasteurianum , 2014, Scientific Reports.

[50]  A. Boetius,et al.  Intercellular wiring enables electron transfer between methanotrophic archaea and bacteria , 2015, Nature.

[51]  Stefano Freguia,et al.  Microbial fuel cells: methodology and technology. , 2006, Environmental science & technology.

[52]  D. Lovley,et al.  Direct Interspecies Electron Transfer between Geobacter metallireducens and Methanosarcina barkeri , 2014, Applied and Environmental Microbiology.

[53]  Bernhard Schink,et al.  Enhanced Propionate Formation by Propionibacterium freudenreichii subsp. freudenreichii in a Three-Electrode Amperometric Culture System , 1990, Applied and environmental microbiology.

[54]  Tian Zhang,et al.  Geobacter: the microbe electric's physiology, ecology, and practical applications. , 2011, Advances in microbial physiology.

[55]  Jean-Philippe Steyer,et al.  Hydrogen production from agricultural waste by dark fermentation: A review , 2010 .

[56]  Kazuya Watanabe,et al.  Microbial interspecies interactions: recent findings in syntrophic consortia , 2015, Front. Microbiol..

[57]  Min Jiang,et al.  Effect of redox potential regulation on succinic acid production by Actinobacillus succinogenes , 2010, Bioprocess and biosystems engineering.

[58]  L. T. Angenent,et al.  Microbial electrochemistry and technology: terminology and classification , 2015 .

[59]  D. Lovley Microbial fuel cells: novel microbial physiologies and engineering approaches. , 2006, Current opinion in biotechnology.