Receptor binding and processing of epidermal growth factor by human breast cancer cells.

Epidermal growth factor (EGF) stimulates the growth of human breast cancer cells (MCF-7). To better define the mechanisms for these effects, we studied receptor binding and subsequent processing of [l25I]EGF in cells growing in monolayer culture under conditions in which an effect on growth is observed. Binding was temperature dependent and was higher at reduced temperature. At 37 C, binding rapidly reached a maximum (20–60 min) and then gradually decreased. Binding was slower at a reduced temperature. High affinity was demonstrated by the ability of unlabeled EGF to compete for [125I]EGF binding; hah7 maximal displacement was observed at an EGF concentration that also resulted in half-maximal biological activity (1–2 ng/ml). Scatchard plots derived from these data or from those of studies using increasing concentrations of [125I]EGF were curilinear and superimposable. Plots from binding data obtained at 0 C were also curvilinear, suggesting that the processing and degradation of tracer alone cannot expla...

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