Optical Characteristics of Greenhouse Plastic Films Affect Yield and Some Quality Traits of Spinach (Spinacia oleracea L.) Subjected to Different Nitrogen Doses

Light and nitrogen strongly affect the growth, yield, and quality of food crops, with greater importance in green leafy vegetables for their tendency to accumulate nitrate in leaves. The purpose of this research was to explore the effect of two greenhouse films (Film A and B) on yield, and quality of spinach grown under different nitrogen regimes (not fertilized—N0%; sub-optimal N dose—N50%; optimal N dose—N100%). Film A and Film B were used as clear and diffused light films, with 75% and 87% thermicity, and 85% and 90% total transmittivity, respectively, where only Film B had a UV-B window. Film B elicited an increase in yield (22%) and soil–plant analysis development (SPAD) index (4.6%) compared to the clear film, but did not affect chlorophyll a, b, and total chlorophyll content. In addition, the diffuse film significantly decreased ascorbic acid in the crop but had no effect on lipophilic antioxidant activity and phenols content, but decreased ascorbic acid content. Finally, nitrate content was strongly increased both by nitrogen dose (about 50-fold more than N0%) and greenhouse films (about six-fold higher under diffuse light film), but within the legal limit fixed by European Commission. Therefore, irrespective of N levels, the use of diffuse-light film in winter boosts spinach yield without depressing quality.

[1]  M. Mori,et al.  Plant-Based Protein Hydrolysate Improves Salinity Tolerance in Hemp: Agronomical and Physiological Aspects , 2021, Agronomy.

[2]  M. Mori,et al.  Effect of seaweed (Ecklonia maxima) extract and legume-derived protein hydrolysate biostimulants on baby leaf lettuce grown on optimal doses of nitrogen under greenhouse conditions , 2020 .

[3]  L. Formisano,et al.  Shading Affects Yield, Elemental Composition and Antioxidants of Perennial Wall Rocket Crops Grown from Spring to Summer in Southern Italy , 2020, Plants.

[4]  S. Grundy,et al.  A Review of Environment Effects on Nitrate Accumulation in Leafy Vegetables Grown in Controlled Environments , 2020, Foods.

[5]  Hou Jiang,et al.  Surface Diffuse Solar Radiation Determined by Reanalysis and Satellite over East Asia: Evaluation and Comparison , 2020, Remote. Sens..

[6]  M. Mori,et al.  The potential of greenhouse diffusing cover material on yield and nutritive values of lamb’s lettuce grown under diverse nitrogen regimes , 2020 .

[7]  Yanwei Hao,et al.  Nutritional quality, mineral and antioxidant content in lettuce affected by interaction of light intensity and nutrient solution concentration , 2020, Scientific Reports.

[8]  M. Mori,et al.  Nitrogen Use and Uptake Efficiency and Crop Performance of Baby Spinach (Spinacia oleracea L.) and Lamb’s Lettuce (Valerianella locusta L.) Grown under Variable Sub-Optimal N Regimes Combined with Plant-Based Biostimulant Application , 2020, Agronomy.

[9]  B. Shahmoradi,et al.  The nitrate content of fresh and cooked vegetables and their health-related risks , 2020, PloS one.

[10]  M. Mori,et al.  Plant-Based Biostimulants Influence the Agronomical, Physiological, and Qualitative Responses of Baby Rocket Leaves under Diverse Nitrogen Conditions , 2019, Plants.

[11]  M. Mori,et al.  Effect of Vegetal- and Seaweed Extract-Based Biostimulants on Agronomical and Leaf Quality Traits of Plastic Tunnel-Grown Baby Lettuce under Four Regimes of Nitrogen Fertilization , 2019, Agronomy.

[12]  S. Pascale,et al.  Assessing the effects of salinity on yield, leaf gas exchange and nutritional quality of spring greenhouse lettuce , 2018, Acta Horticulturae.

[13]  Y. Rouphael,et al.  Nitrate in fruits and vegetables , 2018, Scientia Horticulturae.

[14]  S. Neugart,et al.  UVB and UVA as eustressors in horticultural and agricultural crops , 2018 .

[15]  R. Paradiso,et al.  Morphophysiological Traits and Nitrate Content of Greenhouse Lettuce as Affected by Irrigation with Saline Water , 2017 .

[16]  P. Riga,et al.  Effects of light-diffusing plastic film on lettuce production and quality attributes , 2017 .

[17]  Juan Yu,et al.  Interaction effects of light intensity and nitrogen concentration on growth, photosynthetic characteristics and quality of lettuce (Lactuca sativa L. Var. youmaicai) , 2017 .

[18]  I. Noble,et al.  On the direct effect of clouds and atmospheric particles on the productivity and structure of vegetation , 2001, Oecologia.

[19]  Y. Rouphael,et al.  Nutritional quality of ten leafy vegetables harvested at two light intensities. , 2016, Food chemistry.

[20]  A. Galieni,et al.  Shading and nitrogen management affect quality, safety and yield of greenhouse-grown leaf lettuce , 2015 .

[21]  Tatas H. P. Brotosudarmo,et al.  Analysis on the Chlorophyll Content of Commercial Green Leafy Vegetables , 2015 .

[22]  T. Hirano,et al.  Environmental dependence and seasonal variation of diffuse solar radiation in tropical peatland , 2014 .

[23]  Bupinder Singh,et al.  Enhancement of growth, photosynthetic performance and yield by exclusion of ambient UV components in C3 and C4 plants. , 2013, Journal of photochemistry and photobiology. B, Biology.

[24]  G. Giacomelli,et al.  Influence of the interaction between light intensity and CO2 concentration on productivity and quality of spinach (Spinacia oleracea L.) grown in fully controlled environment , 2013 .

[25]  J. Janse,et al.  Influence of diffuse glass on the growth and production of tomato , 2012 .

[26]  N. Paul,et al.  Ecological responses to UV radiation: interactions between the biological effects of UV on plants and on associated organisms. , 2012, Physiologia plantarum.

[27]  Peter R. J. North,et al.  Control of atmospheric particles on diffuse radiation and terrestrial plant productivity , 2012 .

[28]  Martin Navrátil,et al.  Impact of clear and cloudy sky conditions on the vertical distribution of photosynthetic CO2 uptake within a spruce canopy , 2012 .

[29]  W. L. Zhou,et al.  Quality changes in hydroponic lettuce grown under pre-harvest short-duration continuous light of different intensities , 2012 .

[30]  Nilton Nélio Cometti,et al.  Nitrate concentration in lettuce leaves depending on photosynthetic photon flux and nitrate concentration in the nutrient solution , 2011 .

[31]  Muhammad Iqbal,et al.  Nitrate accumulation in plants, factors affecting the process, and human health implications. A review , 2011, Agronomy for Sustainable Development.

[32]  N. Bryan,et al.  Nutritional epidemiology in the context of nitric oxide biology: a risk-benefit evaluation for dietary nitrite and nitrate. , 2010, Nitric oxide : biology and chemistry.

[33]  Tarek G. Ammari,et al.  Nitrate accumulation in spinach (Spinacia oleracea L.) tissues under different fertilization regimes , 2010 .

[34]  H. Yoshida,et al.  Spinach and Health , 2010 .

[35]  Zhujun Zhu,et al.  Effects of Nitrogen and Sulfur on Total Phenolics and Antioxidant Activity in Two Genotypes of Leaf Mustard , 2008 .

[36]  A. Souza,et al.  Nitrate production and accumulation in lettuce as affected by mineral Nitrogen supply and organic fertilization , 2008 .

[37]  F. Stagnari,et al.  Effects of N fertilizers and rates on yield, safety and nutrients in processing spinach genotypes , 2007 .

[38]  C. Lister Nutritional attributes of spinach , silver beet and eggplant , 2007 .

[39]  N. Katsoulas,et al.  Effect of two UV-absorbing greenhouse-covering films on growth and yield of an eggplant soilless crop , 2006 .

[40]  P. Santamaria Nitrate in vegetables: toxicity, content, intake and EC regulation , 2006 .

[41]  M. Iqbal,et al.  USE OF VEGETABLES AS NUTRITIONAL FOOD: ROLE IN HUMAN HEALTH , 2006 .

[42]  N. Gruda Impact of Environmental Factors on Product Quality of Greenhouse Vegetables for Fresh Consumption , 2005 .

[43]  A. Steiner,et al.  Aerosol-induced thermal effects increase modelled terrestrial photosynthesis and transpiration , 2005 .

[44]  T. Breimer Environmental factors and cultural measures affecting the nitrate content in spinach , 1982, Fertilizer research.

[45]  Lee Siyoung,et al.  Environments and canopy productivity of green pepper (Capsicum annuum L.) in a greenhouse using light-diffused woven film. , 2005 .

[46]  U. Praeger,et al.  PRECISION STRESSING BY UV-B RADIATION TO IMPROVE QUALITY OF SPINACH UNDER PROTECTED CULTIVATION , 2004 .

[47]  W. Schnitzler,et al.  EFFECT OF PAR AND UV-B RADIATION ON THE QUALITY AND QUANTITY OF THE ESSENTIAL OIL IN SWEET BASIL (OCIMUM BASILICUM L.) , 2004 .

[48]  D. Vodnik,et al.  The Effect of Light-dependent Application of Nitrate on the Growth of Aeroponically Grown Lettuce (Lactuca sativa L.) , 2004 .

[49]  W. Weerakkody Nutritional Value of Fresh Leafy Vegetables as Affected by Pre-harvest Factors , 2003 .

[50]  P. Brandt,et al.  Nitrite and potential endogenous formation of N-nitroso compounds; Safety evaluation of certain food additives, JECFA , 2003 .

[51]  N. Smirnoff Ascorbate biosynthesis and function in photoprotection. , 2000, Philosophical transactions of the Royal Society of London. Series B, Biological sciences.

[52]  Y. Shinohara,et al.  Effects of Air Temperature and Light Intensity on β-carotene Concentration in Spinach and Lettuce , 1999 .

[53]  M. Peet GREENHOUSE CROP STRESS MANAGEMENT , 1999 .

[54]  C. Rice-Evans,et al.  [34] Screening of dietary carotenoids and carotenoid-rich fruit extracts for antioxidant activities applying 2,2′-azinobis(3-ethylenebenzothiazoline-6-sulfonic acid radical cation decolorization assay , 1999 .

[55]  R. Lamuela-Raventós,et al.  Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent , 1999 .

[56]  Victor Gaba,et al.  Higher plants and UV-B radiation: balancing damage, repair and acclimation , 1998 .

[57]  Y. P. Abrol,et al.  Exclusion of UV-B radiation from normal solar spectrum on the growth of mung bean and maize , 1997 .

[58]  D. Inzé,et al.  Extraction and determination of ascorbate and dehydroascorbate from plant tissue. , 1995, Analytical biochemistry.

[59]  J B Kaneene,et al.  The effects of nitrate, nitrite and N-nitroso compounds on human health: a review. , 1993, Veterinary and human toxicology.

[60]  M. Hannah,et al.  Light Interception and Photosynthetic Efficiency in Some Glasshouse Crops , 1992 .

[61]  Gary E. Weir,et al.  The Department of Energy , 1989 .

[62]  K. Goh,et al.  Effects of fertilisers on vegetable production 2. Effects of nitrogen fertilisers on nitrogen content and nitrate accumulation of spinach and beetroot , 1986 .

[63]  A. Wellburn,et al.  Determinations of total carotenoids and chlorophylls a and b of leaf extracts in different solvents , 1983 .

[64]  A. Barker,et al.  Nutritional factors affecting nitrate accumulation in spinach , 1971 .