Analysis of Immunoglobulin A Antibodies toHelicobacter pylori in Serum and Gastric Juice in Relation to Mucosal Inflammation

ABSTRACT Helicobacter pylori is a major etiologic agent in gastroduodenal disorders. In this study, immunoglobulin A (IgA) antibodies to H. pylori antigens were evaluated in serum and gastric juice specimens obtained from patients with gastritis or peptic ulcers by utilizing antibody capture enzyme-linked immunosorbent assays (ACELISAs). Urease α subunit (UA), urease β subunit (UB), the 66-kDa heat shock protein (HSP), and the 25-kDa protein (25K) were used as antigens for the ACELISAs. The antibody titers of the ACELISAs reflect the ratio of H. pylori-specific IgA to total IgA. The ratio is stable, although the antibody concentration fluctuates in gastric juice. By using ACELISAs it was possible to evaluate quantitatively not only serum IgA antibodies but also gastric juice secretory IgA (S-IgA) antibodies. In both serum IgA and gastric juice S-IgA ACELISAs, the titers of antibody to HSP and 25K were remarkably correlated with the histologic grade of gastritis, whereas those to UA and UB were not strongly correlated with histologic grade. Thus, it is useful for estimating the histologic grade of gastritis to quantify serum IgA and gastric juice S-IgA antibodies to HSP and 25K.

[1]  T. Sugiyama,et al.  Mucosal immune response to Helicobacter pylori and cytotoxic mechanism. , 1995, Scandinavian journal of gastroenterology. Supplement.

[2]  B. Coffin,et al.  Secretion of immunoglobulins and plasma proteins from the colonic mucosa: an in vivo study in man , 1995, Clinical and experimental immunology.

[3]  R. Williams,et al.  Inhibition of Bacterial Adherence by Secretory Immunoglobulin A: A Mechanism of Antigen Disposal , 1972, Science.

[4]  M. Dixon,et al.  Chronic gastritis—a pathogenetic approach , 1988, The Journal of pathology.

[5]  J. Sever,et al.  Rapid detection of herpes simplex virus in clinical specimens by use of a capture biotin-streptavidin enzyme-linked immunosorbent assay , 1984, Journal of clinical microbiology.

[6]  J. Hodgson,et al.  Evaluation of a commercial antibody capture enzyme immunoassay for the detection of rubella specific IgM. , 1984, Journal of clinical pathology.

[7]  A. Kai,et al.  Isolation of Campylobacter pyloridis from Human Gastric Mucosa and Characterization of the Isolates , 1987, Microbiology and immunology.

[8]  S. Czinn,et al.  Diagnosis of gastritis caused by Helicobacter pylori in children by means of an ELISA. , 1991, Reviews of infectious diseases.

[9]  D. Burke,et al.  Antibody Capture Immunoassay Detection of Japanese Encephalitis Virus Immunoglobulin M and G Antibodies in Cerebrospinal Fluid , 1982, Journal of clinical microbiology.

[10]  K. Yokota,et al.  A novel enzyme immunoassay for serodiagnosis of Helicobacter pylori infection. , 1991, Gastroenterology.

[11]  T. Monath,et al.  Oral immunization with recombinant Helicobacter pylori urease induces secretory IgA antibodies and protects mice from challenge with Helicobacter felis. , 1995, The Journal of infectious diseases.

[12]  T. Sugiyama,et al.  Heat Shock Protein Produced by Helicobacter pylori , 1994, Microbiology and immunology.

[13]  A. Blum,et al.  Immunization of BALB/c mice against Helicobacter felis infection with Helicobacter pylori urease. , 1994, Gastroenterology.

[14]  A. Moran,et al.  Identification of the N-acetylneuraminyllactose-specific laminin-binding protein of Helicobacter pylori , 1997, Infection and immunity.

[15]  A. Yachi,et al.  The IgA Type Parietal Cell Antibody in Gastric Juice , 1985 .

[16]  T. Sugiyama,et al.  Quantitative detection of secretory immunoglobulin A to Helicobacter pylori in gastric juice: Antibody capture enzyme‐linked immunosorbent assay , 1996, Journal of clinical laboratory analysis.

[17]  C. Vincent,et al.  Serum and bile secretory immunoglobulins and secretory component during the early postoperative course after liver transplantation , 1991, Hepatology.

[18]  R. J. Owen,et al.  RAPID UREA HYDROLYSIS BY GASTRIC CAMPYLOBACTERS , 1985, The Lancet.

[19]  S. Ichiyama,et al.  Interleukin-8 activity correlates with histological severity in Helicobacter pylori-associated antral gastritis. , 1996, The American journal of gastroenterology.

[20]  J. Biewenga,et al.  Albumin and immunoglobulin levels in nasal secretions of patients with nasal polyps treated with endoscopic sinus surgery and topical corticosteroids. , 1995, The Journal of allergy and clinical immunology.

[21]  J. Fox,et al.  Effect of oral immunization with recombinant urease on murine Helicobacter felis gastritis , 1995, Infection and immunity.

[22]  T. A. S. Group.  An international association between Helicobacter pylori infection and gastric cancer , 1993, The Lancet.

[23]  M. Blaser,et al.  Correlation between serological and mucosal inflammatory responses to Helicobacter pylori. , 1994, Clinical and diagnostic laboratory immunology.

[24]  C. Mcnulty,et al.  RAPID DIAGNOSIS OF CAMPYLOBACTER-ASSOCIATED GASTRITIS , 1985, The Lancet.

[25]  C. Self,et al.  Enzyme amplification--a general method applied to provide an immunoassisted assay for placental alkaline phosphatase. , 1985, Journal of immunological methods.

[26]  T. Sugiyama,et al.  The effect of the immune response to Helicobacter pylori in the development of intestinal metaplasia. , 1994, European Journal of Gastroenterology and Hepathology.

[27]  S. Czinn,et al.  Protection of germ-free mice from infection by Helicobacter felis after active oral or passive IgA immunization. , 1993, Vaccine.

[28]  C H Self,et al.  Enzyme amplification can enhance both the speed and the sensitivity of immunoassays. , 1985, Journal of immunological methods.

[29]  B. Marshall,et al.  UNIDENTIFIED CURVED BACILLI ON GASTRIC EPITHELIUM IN ACTIVE CHRONIC GASTRITIS , 1983, The Lancet.

[30]  D. Graham.  I. Helicobacter pylori: Its epidemiology and its role in duodenal ulcer disease , 1991, Journal of gastroenterology and hepatology.

[31]  R. Negrini,et al.  Helicobacter pylori infection induces antibodies cross-reacting with human gastric mucosa. , 1991, Gastroenterology.

[32]  J. Pfeifer,et al.  Paracrystalline inclusions of a novel ferritin containing nonheme iron, produced by the human gastric pathogen Helicobacter pylori: evidence for a third class of ferritins , 1993, Journal of bacteriology.

[33]  Mark Borodovsky,et al.  The complete genome sequence of the gastric pathogen Helicobacter pylori , 1997, Nature.