ANTIBACTERIAL AND ANTIOXIDANT ACTIVITIES OF THE TISSUE EXTRACT OF PERNA VIRIDIS LINNAEUS, 1758 (MOLLUSCA: BIVALVIA) FROM VERSOVA COAST, MUMBAI

Objective: The study of marine organism for their bioactive potential, being a significant part of marine ecosystem has picked up the regularity in recent years with the growth recognition of their importance in human life as well as animals. Methods: In this present study methanol tissue extract of edible green mussel (P. viridis) was assayed for the antibacterial activity against six bacterial pathogens and antioxidant activities also determined. Results: The antibacterial activity of P. viridis tissue extract, showed maximum zone of inhibition (15mm) against Vibrio cholerae and minimum activity (5 mm) was observed in Klebsiella pneumoniae. Molecular size of green mussel protein was determined using Sodiumdodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). FTIR analysis reveals the presence of bioactive compounds signals at different ranges. The antioxidant activity of crude protein tissue extract from the P. viridis were measured in different system of assay such as DPPH assay (76.9%), total reducing power (27.8 μg), total antioxidant activity (174 μg), hydrogen peroxide scavenging (88.12 %) and nitrous oxide scavenging activity (62.5%) at 100μg/ml. Conclusion: crude tissue extract from P. viridis could be effectively used as alternative source of antimicrobial and antioxidant with subsequent health benefits.

[1]  A. Garateix,et al.  Bioactive peptides from marine sources: pharmacological properties and isolation procedures. , 2004, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences.

[2]  T. Singh,et al.  Purification of Anti-HIV Protein from Purple Fluid of the Sea Hare Bursatella leachii de Blainville , 2002, Marine Biotechnology.

[3]  T. P. Anand,et al.  Antimicrobial activity in the tissue extracts of five species of cowries Cypraea spp. (Mollusca: Gastropoda) and an ascidian Didemnum psammathodes (Tunicata: Didemnidae) , 2002 .

[4]  G. Schwartsmann,et al.  Natural products in anticancer therapy. , 2001, Current opinion in pharmacology.

[5]  R. Berlinck,et al.  Marine organisms as a source of new anticancer agents. , 2001, The Lancet. Oncology.

[6]  V. Agmon,et al.  Emergence of multidrug-resistant Salmonella enterica serotype Typhimurium phage-type DT104 among salmonellae causing enteritis in Israel , 1998, Epidemiology and Infection.

[7]  M. Úriz,et al.  Antimicrobial activity and surface bacterial film in marine sponges , 1994 .

[8]  U. K. Laemmli,et al.  Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4 , 1970, Nature.

[9]  Oliver H. Lowry,et al.  Protein measurement with the Folin phenol reagent. , 1951, The Journal of biological chemistry.

[10]  E. Dilipan,et al.  ANTIMICROBIAL PEPTIDE FROM CRAB HAEMOLYPMH OF OCYPODA MACROCERA (Milne Edwards 1852) WITH REFERENCE TO ANTIOXIDANT: A CASE STUDY , 2013 .

[11]  Wael E Houssen,et al.  Isolation of marine natural products. , 2012, Methods in molecular biology.

[12]  Sumita Sharma,et al.  Effect of Different Extraction Procedures on Antimicrobial Activity of Marine Bivalves: A Comparison , 2009 .

[13]  D. Faulkner,et al.  Marine pharmacology , 2004, Antonie van Leeuwenhoek.

[14]  D. Faulkner,et al.  Highlights of marine natural products chemistry (1972-1999). , 2000, Natural product reports.

[15]  A. Wright.  Isolation of Marine Natural Products , 1998 .

[16]  G. Torri,et al.  Structural differences between chitin polymorphs and their precipitates from solutions—evidence from CP-MAS 13C-NMR, FT-IR and FT-Raman spectroscopy , 1992 .

[17]  S. Løvtrup,et al.  Acid mucopolysaccharides in the skin of some lower vertebrates (hagfish, lamprey and Chimaera). , 1976, Comparative biochemistry and physiology. B, Comparative biochemistry.

[18]  S. Orr.  Infra-red spectroscopic studies of some polysaccharides. , 1954, Biochimica et biophysica acta.