Immobilization of Lipases on Different Carriers and Their Use in Synthesis of Pentyl Isovalerates

Abstract Porcine pancreatic lipase (PPL) and Candida cylindracea lipase (CCL) were immobilized on Celite and Amberlite IRA 938 by deposition from the aqueous solution by the addition of hexane. The influence of the immobilization on the activities of the immobilized lipase derivatives has been studied. The immobilized lipases were used in synthesis of pentyl isovalerates. Various reaction parameters affecting the synthesis of pentyl isovalerates were investigated. The reaction rates were compared with the rates of esterification with free lipases. The immobilized lipases were found to be very effective in the esterification reaction. The lipases immobilized on Celite 545 exhibited better operational stabilities than that of immobilized on Amberlite IRA‐938.

[1]  P. Adlercreutz,et al.  Immobilisation of lipases by adsorption and deposition: high protein loading gives lower water activity optimum , 2000, Biotechnology Letters.

[2]  D. Frense,et al.  Immobilization of Candida rugosa lipase in lyotropic liquid crystals and some properties of the immobilized enzyme , 1996, Biotechnology Letters.

[3]  Zheng Guo,et al.  Preparation and characterization of immobilized lipase on magnetic hydrophobic microspheres , 2003 .

[4]  M. Matsumoto,et al.  Effect of immobilization on thermostability of lipase from Candida rugosa , 2003 .

[5]  A. Kılınç,et al.  Chemical attachment of porcine pancreatic lipase to crosslinked poly(vinyl alcohol) by means of adipoyldichloride , 2002 .

[6]  Sajja Hari Krishna,et al.  Developments and trends in enzyme catalysis in nonconventional media. , 2002, Biotechnology advances.

[7]  N. S. Dosanjh,et al.  Immobilization, stability and esterification studies of a lipase from a Bacillus sp. , 2002, Biotechnology and applied biochemistry.

[8]  M. Reetz.  Lipases as practical biocatalysts. , 2002, Current opinion in chemical biology.

[9]  G. Pencréac’h,et al.  Comparison of hydrolytic activity in water and heptane for thirty-two commercial lipase preparations. , 2001, Enzyme and microbial technology.

[10]  A. Kiener,et al.  Industrial biocatalysis today and tomorrow , 2001, Nature.

[11]  A. Klibanov.  Improving enzymes by using them in organic solvents , 2001, Nature.

[12]  H. F. Castro,et al.  Immobilisation studies and catalytic properties of microbial lipase onto styrene–divinylbenzene copolymer , 2000 .

[13]  G. Zanin,et al.  Immobilization of porcine pancreatic lipase on celite for application in the synthesis of butyl butyrate in a nonaqueous system , 1999 .

[14]  R. Verger,et al.  Lipases: Interfacial Enzymes with Attractive Applications. , 1998, Angewandte Chemie.

[15]  M. Schneider,et al.  Methods for the immobilization of lipases and their use for ester synthesis , 1997 .

[16]  L. M. Simon,et al.  Immobilization and characterization of porcine pancreas lipase , 1997 .

[17]  C. Akoh,et al.  Enzymatic synthesis of geranyl acetate inn-hexane withCandida antarctica lipases , 1994 .

[18]  K. Poutanen,et al.  Applications of immobilized lipases to transesterification and esterification reactions in nonaqueous systems. , 1993, Enzyme and microbial technology.

[19]  A. Telefoncu,et al.  SYNTHESIS OF STEREOSPECIFIC ESTERS AND RESOLUTION OF RACEMIC ALCOHOLS WITHIMMOBILIZED LIPASES , 1993 .

[20]  A. Telefoncu,et al.  Preparation and characterization of pancreatic lipase immobilized in Eudragit-matrix , 1990, Applied biochemistry and biotechnology.

[21]  C. Laane,et al.  Optimization of biocatalysis in organic media. , 1987 .

[22]  A. Meffert.  Technical uses of fatty acid esters , 1984 .

[23]  O. H. Lowry,et al.  Protein measurement with the Folin phenol reagent. , 1951, The Journal of biological chemistry.