Western blot is an efficient tool in differential diagnosis 1 between paracoccidioidomycosis and pulmonary tuberculosis 2 3

3 Thâmara Aline Bertoni Maysa Cláudia Zolin Perenha-Viana, Eliana Valéria Patussi, 4 Rosilene Fressatti Cardoso, Terezinha Inez Estivalet Svidzinski. 5 6 Laboratory of Medical Mycology, Department of Clinical Analysis, Maringá, PR, 7 Brazil, Laboratory of Micobateria, Department of Clinical Analysis, Maringá, PR, 8 Brazil. Correspondence author: terezinha.svidzinski@gmail.com 9 10 Running title: differential diagnosis between PCM and TB 11 12 Mailing Address: T. I. E. Svidzinski, Ph.D. Medical Mycology Laboratory, Clinical 13 Analysis Department, Universidade Estadual de Maringá, Maringá, Paraná State, Brazil. 14 87020-900. Tel: +55 44 3011-4809. E-mail: terezinha.svidzinski@gmail.com 15 16 ABSTRACT 17 Sputum and sera from 134 patients screened for tuberculosis (TB) were analyzed to 18 investigate TB and paracoccidioidomycosis (PCM). Of these patients, 11 (8.2%) were 19 confirmed to have TB, but six (4.5%) were positive only for PCM. All patients with 20 PCM presented anti 43KDa component antibodies in Western Blot (WB), while in the 21 TB-positive patients it did not appear. This preliminary study suggests WB as a 22 potential tool for the differential laboratory diagnosis between TB and PCM. 23 24 Key-words: Paracoccidioidomycosis. Tuberculosis. Differential diagnosis. Serology 25 Copyright © 2012, American Society for Microbiology. All Rights Reserved. Clin. Vaccine Immunol. doi:10.1128/CVI.00252-12 CVI Accepts, published online ahead of print on 12 September 2012

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