Procollagen III peptide (PIIIP): Can it reflect hepatic fibrosis?

Immunolocalization of type III collagen and procollagen in cirrhotic human liver was studied using monoclonal antibody specific for the helical determinant of type III collagen extracted from human placenta. Deparaffinized, trypsin‐treated cirrhotic liver sections from 8 autopsy cases were examined by the unlabeled peroxidase‐antiperoxidase and immunofluorescence techniques. These techniques revealed the localization of this epitope shared by type III collagen and procollagen not only in the extracellular matrix of hepatocytes and sinusoidal cells but also in the cytoplasm. In hepatocellular carcinoma concurrent with cirrhosis, neoplastic cells were shown to react with this antibody as well.

[1]  J. Davies.  Pathology annual volume 21, part 2. S. C. Sommers, P. P. Rosen, R. E. Fechner (Eds). Appleton‐Century‐Crofts, Norwalk, Connecticut, USA, 1986. No. of pages: vii + 377. Price: £64.15. ISBN: 0 8385 7777 6 , 1987 .

[2]  R. Timpl,et al.  Type III collagen aminopropeptide levels in serum of patients with progressive systemic scleroderma. , 1986, The Journal of investigative dermatology.

[3]  W. Franke,et al.  Change of cytokeratin organization during development of Mallory bodies as revealed by a monoclonal antibody. , 1986, Laboratory investigation; a journal of technical methods and pathology.

[4]  H. Denk,et al.  Cytoskeleton in Liver Diseases , 1986, Seminars in liver disease.

[5]  C. Lieber,et al.  Ultrastructural localization of type III procollagen in baboon liver. , 1986, The American journal of pathology.

[6]  A. Zimmermann,et al.  The N‐Terminal Propeptide of Collagen Type III in Serum Reflects Activity and Degree of Fibrosis in Patients with Chronic Liver Disease , 1984, Hepatology.

[7]  B. Kommerell,et al.  Biliary excretion of procollagen type III peptide in healthy humans and in patients with alcoholic cirrhosis of the liver. , 1983, Gastroenterology.

[8]  J. Risteli,et al.  Aminoterminal propeptide of type III procollagen in serum in alcoholic liver disease , 1983 .

[9]  K. Cutroneo,et al.  Lavage type III procollagen N-terminal peptides in human pulmonary fibrosis and sarcoidosis. , 1983, Laboratory investigation; a journal of technical methods and pathology.

[10]  I. Murray-Lyon,et al.  NATURAL HISTORY OF LIVER DISEASE IN CHRONIC HEPATITIS B SURFACE ANTIGEN CARRIERS Survey of 100 Patients from Great Britain , 1981, The Lancet.

[11]  W. Franke,et al.  Constitutive aggregates of intermediate-sized filaments of the vimentin and cytokeratin type in cultured hepatoma cells and their dispersal by butyrate. , 1980, Experimental cell research.

[12]  R. Timpl,et al.  Radioimmunoassay for type III procollagen peptide and its application to human liver disease , 1979, European journal of clinical investigation.

[13]  B. Davis,et al.  Type I and type III procollagen peptides during hepatic fibrogenesis. An immunohistochemical and ELISA serum study in the CCl4 rat model. , 1987, The American journal of pathology.

[14]  R. Wiesner,et al.  Serum type III procollagen peptide concentrations in severe chronic active hepatitis: Relationship to cirrhosis and disease activity , 1987, Hepatology.

[15]  R. Wiesner,et al.  Serial determinations of the amino-terminal peptide of type III procollagen in severe chronic active hepatitis. , 1987, The Journal of laboratory and clinical medicine.

[16]  K. Kovitz,et al.  Serum levels of type I and III procollagen fragments in Paget's disease of bone. , 1984, The Journal of clinical endocrinology and metabolism.

[17]  S. French.  Present understanding of the development of Mallory's body. , 1983, Archives of pathology & laboratory medicine.