Chicken infectious anemia in Mexico: virus identification and serology survey.

Three chicken infectious anemia (CIA) virus strains were isolated from 10 different sick broiler and replacement chicken flocks with the MDCC-MSB1 cell line. One-day-old specific-pathogen-free chicks were inoculated later, with the three original samples being positive in tissue culture; one induced signs and lesions, another only lesions typical for CIA. One isolate was selected for further trials and showed resistance to chloroform and heat (75 C for 5 min) and passed through a 45-nm filter membrane but did not pass through the 22-nm filter. These characteristics were similar to the Del Rose reference strain of chicken anemia virus. By electron microscopy, the diameter of particles obtained from the pellet of infected cell cultures was between 22 and 27 nm. Serology survey carried out with 580 serum samples from different poultry farms all over the country with a commercial enzyme-linked immunosorbent assay kit gave proof of widespread seroconversion, indicating that CIA should be considered endemic to Mexico.

[1]  A. Coombes,et al.  Recommended storage and resuscitation conditions for the MDCC-MSB1 cell line. , 1998, Avian diseases.

[2]  N. Saubí,et al.  Experimental evaluation of an inactivated vaccine against chicken anaemia virus. , 1997, Avian pathology : journal of the W.V.P.A.

[3]  T. Campbell Avian Hematology and Cytology , 1995 .

[4]  J. Wicht,et al.  Chicken anaemia agent in South Africa , 1993, Veterinary Record.

[5]  D. Mackie,et al.  A sequential histopathologic and immunocytochemical study of chicken anemia virus infection at one day of age. , 1993, Avian diseases.

[6]  S. Taylor The effect of acetone on the viability of chicken anemia agent. , 1992, Avian diseases.

[7]  É. Sághy,et al.  Isolation of chicken anaemia virus from broiler chickens. , 1992, Acta veterinaria Hungarica.

[8]  C. Pope,et al.  Chicken anemia agent. , 1991, Veterinary immunology and immunopathology.

[9]  N. Morés,et al.  Isolation and Identification of Chicken Infectious Anemia Virus in China , 1991 .

[10]  M. Mcnulty Chicken anaemia agent: a review. , 1991, Avian pathology : journal of the W.V.P.A.

[11]  M. Mcnulty,et al.  Economic effects of subclinical chicken anemia agent infection in broiler chickens. , 1991, Avian diseases.

[12]  G. Firth,et al.  Isolation of chicken anaemia agent from Australian poultry. , 1990, Australian Veterinary Journal.

[13]  D. Mackie,et al.  Chicken anemia agent: an electron microscopic study. , 1990, Avian diseases.

[14]  K. Schat,et al.  Identification of the chicken anemia agent, reproduction of the disease, and serological survey in the United States. , 1990, Avian diseases.

[15]  F. McNeilly,et al.  Preliminary characterisation of isolates of chicken anaemia agent from the United Kingdom. , 1990, Avian pathology : journal of the W.V.P.A.

[16]  J. Rosenberger,et al.  The isolation and characterization of chicken anemia agent (CAA) from broilers in the United States. , 1989, Avian diseases.

[17]  T. Umemura,et al.  Histopathology of chicks inoculated with chicken anaemia agent (MSB1-TK5803 strain). , 1989, Avian pathology : journal of the W.V.P.A.

[18]  H. Landgraf,et al.  Anaemia-dermatitis of broilers: field observations on its occurrence, transmission and prevention. , 1988, Avian pathology : journal of the W.V.P.A.

[19]  O. Fossum,et al.  Blue wing disease of chickens: experimental infection with a Swedish isolate of chicken anaemia agent and an avian reovirus. , 1988, Avian pathology : journal of the W.V.P.A.

[20]  T. Umemura,et al.  Serial propagation and purification of chicken anaemia agent in MDCC-MSB1 cell line. , 1987, Avian pathology : journal of the W.V.P.A.

[21]  T. Umemura,et al.  Isolation of an agent inducing chicken anaemia. , 1985, Avian pathology : journal of the W.V.P.A.

[22]  T. Taniguchi,et al.  Isolation and some characteristics of an agent inducing anemia in chicks. , 1979 .

[23]  C. N. Burke,et al.  A manual of basic virological techniques , 1973 .