Removal of synthetic dyes by an eco‐friendly strategy

In this paper an eco‐friendly strategy for the removal of synthetic dyes from aqueous solutions was proposed. Thus, in a first step the dyes were adsorbed onto sunflower seed shells (SS) by using a batch technique. Subsequently, in a second step, these dyed SS were used as support‐substrates to produce laccase by the white‐rot fungus Trametes pubescens under semi‐solid‐state conditions. The effect of inducer addition on laccase production was studied. The optimum conditions (addition of both 0.5 mM Cu+2 and 50 μM tannic acid on the 3rd day of cultivation) led to a maximum laccase activity of 30272 U/L. Further, the system was efficiently scaled‐up to laboratory bioreactors producing a maximum activity of 40172 U/L together with a total decolouration of the adsorbed dye. In addition, SDS‐PAGE showed that laccases were the only enzymes present in the extracellular fluid. Therefore, apart from dye removal this approach allowed the production of high titres of laccase, which was obtained almost pure (only needed to be ultra‐filtrated).

[1]  J. Toca-Herrera,et al.  Sunflower seed shells: a novel and effective low-cost adsorbent for the removal of the diazo dye Reactive Black 5 from aqueous solutions. , 2007, Journal of hazardous materials.

[2]  S. R. Couto,et al.  Mandarin peelings: the best carbon source to produce laccase by static cultures of Trametes pubescens. , 2007, Chemosphere.

[3]  S. R. Couto,et al.  Banana skin: A novel waste for laccase production by Trametes pubescens under solid-state conditions. Application to synthetic dye decolouration , 2007 .

[4]  Jurg Keller,et al.  Optimization of integrated chemical-biological degradation of a reactive azo dye using response surface methodology. , 2006, Journal of hazardous materials.

[5]  A. R. Binupriya,et al.  Biosorption of Metal Ions from Aqueous Solution and Electroplating Industry Wastewater by Aspergillus japonicus: Phytotoxicity Studies , 2006, Bulletin of environmental contamination and toxicology.

[6]  C. Galli,et al.  New mediators for the enzyme laccase: mechanistic features and selectivity in the oxidation of non-phenolic substrates , 2005 .

[7]  M. A. Sanromán,et al.  Stainless steel sponge: a novel carrier for the immobilisation of the white-rot fungus Trametes hirsuta for decolourization of textile dyes. , 2004, Bioresource technology.

[8]  S. Shleev,et al.  Comparison of physico-chemical characteristics of four laccases from different basidiomycetes. , 2004, Biochimie.

[9]  L. Viikari,et al.  Purification, characterization and sequence analysis of a laccase from the ascomycete Mauginiella sp. , 2003 .

[10]  Sanjeev Chaudhari,et al.  Decolorization of indigo and azo dyes in semicontinuous reactors with long hydraulic retention time , 2003 .

[11]  A. Demirbas,et al.  Fuel analyses of selected oilseed shells and supercritical fluid extraction in alkali medium , 2002 .

[12]  W. Steiner,et al.  Production of laccase by a newly isolated strain of Trametes modesta. , 2002, Bioresource technology.

[13]  B. Hinterstoisser,et al.  Increased production of laccase by the wood-degrading basidiomycete Trametes pubescens , 2002 .

[14]  P. Baldrian,et al.  Copper and cadmium increase laccase activity in Pleurotus ostreatus. , 2002, FEMS microbiology letters.

[15]  G. Marino,et al.  Purification, Characterization, and Functional Role of a Novel Extracellular Protease from Pleurotus ostreatus , 2001, Applied and Environmental Microbiology.

[16]  E. Srebotnik,et al.  Degradation of nonphenolic lignin by the laccase/1-hydroxybenzotriazole system. , 2000, Journal of biotechnology.

[17]  A. Pilosof,et al.  Biomass estimation in solid state fermentation by modeling dry matter weight loss , 1999 .

[18]  P. Collins,et al.  Regulation of Laccase Gene Transcription in Trametes versicolor , 1997, Applied and environmental microbiology.

[19]  C. Vergoignan,et al.  Biomass estimation in solid state fermentation , 1997 .

[20]  L. Raaska,et al.  Detection of white-rot fungi by a non-toxic stain , 1990 .

[21]  A. Leonowicz,et al.  Comparative Studies of Extracellular Fungal Laccases , 1984, Applied and environmental microbiology.

[22]  M. Kuwahara,et al.  Separation and characterization of two extracelluar H2O2‐dependent oxidases from ligninolytic cultures of Phanerochaete chrysosporium , 1984 .

[23]  M. Tien,et al.  Lignin-degrading enzyme from Phanerochaete chrysosporium: Purification, characterization, and catalytic properties of a unique H(2)O(2)-requiring oxygenase. , 1984, Proceedings of the National Academy of Sciences of the United States of America.

[24]  C. Ginther.  Sporulation and the Production of Serine Protease and Cephamycin C by Streptomyces lactamdurans , 1979, Antimicrobial Agents and Chemotherapy.

[25]  U. K. Laemmli,et al.  Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4 , 1970, Nature.