Quantitative analysis of total phenolic, flavonoid contents and HPTLC fingerprinting of flower extracts of Phlogacanthus thyrsiflorus Nees

Objective: To quantify total phenolic, flavonoid contents in Phlogacanthus thyrsiflorus to evaluate the antioxidant potential along with HPTLC fingerprinting for identification of phytocompounds present in this medicinal plant. Methods: Phytochemical screening, content of total phenolics and flavonoids were determined. The HPTLC fingerprinting of methanolic flower extract (MFE) of the Phlogacanthus thyrsiflorus was also carried out using CAMAG-HPTLC system. Results: Phytochemical investigations revealed the presence of alkaloids, terpenes, tannins, saponins, glycosides and flavonoids. Total phenolic and flavonoid contents were found to be 65 μg/mg and 46 μg/mg, respectively. HPTLC analysis of MFE revealed numerous bands, indicating the presence of diverse groups of phytocompounds and also confirmed naringenin and ascorbic acid in the MFE sample. Conclusions: It can be concluded that HPTLC fingerprint analysis of MFE of Phlogacanthus thyrsiflorus can be used as a diagnostic tool for the correct identification of the plant and it is also useful as a phytochemical marker.

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