CALCOFLUOR AS A FLUORESCENT PROBE TO DETECT BIOFILMS OF FOODBORNE PATHOGENS

Biofilms enable foodborne pathogens to resist removal from surfaces, survive disinfection and elude detection. This study evaluated the use of Calcofluor, which binds to polysaccharides containing β-D-glucans, to detect biofilms produced by Salmonella enterica serovar Berta and Salmonella enterica serovar Typhimurium DT104 (St DT104), Escherichia coli, Aeromonas hydrophila, Vibrio cholerae O139 and Hyphomonas adhaerens. Biofilms produced by St DT104, S. berta and V. cholerae on five types of surfaces (glass, polypropylene, Teflon™, stainless steel and aluminum) were detected by Calcofluor. Results suggest the potential use of Calcofluor as probes of foodborne pathogens in biofilms.

[1]  R. Weiner,et al.  Salmonella enterica Serovar Typhimurium DT104 Displays a Rugose Phenotype , 2001, Applied and Environmental Microbiology.

[2]  N. Tablante,et al.  A Management Technique to Identify Prime Locations of Salmonella Contamination on Broiler and Layer Farms , 2000 .

[3]  S. Joseph,et al.  Improved Plating Media for the Detection of Salmonella Species with Typical and Atypical Hydrogen Sulfide Production , 2000, Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc.

[4]  P. Bremer,et al.  The influence of welding procedures on bacterial colonization of stainless steel weldments , 1999 .

[5]  S. Wai,et al.  Vibrio cholerae O1 Strain TSI-4 Produces the Exopolysaccharide Materials That Determine Colony Morphology, Stress Resistance, and Biofilm Formation , 1998, Applied and Environmental Microbiology.

[6]  C. Kumar,et al.  Significance of microbial biofilms in food industry: a review. , 1998, International journal of food microbiology.

[7]  R. Weiner,et al.  Evidence for the Adhesive Function of the Exopolysaccharide of Hyphomonas Strain MHS-3 in Its Attachment to Surfaces , 1995, Applied and environmental microbiology.

[8]  D. Maneval,et al.  Preliminary structure determination of the capsular polysaccharide of Vibrio cholerae O139 Bengal Al1837 , 1995, Journal of bacteriology.

[9]  E. Russek-Cohen,et al.  Correlation of water activity and other environmental conditions with repeated detection of Salmonella contamination on poultry farms. , 1992, Avian diseases.

[10]  I. Goldstein,et al.  What should be called a lectin? , 1980, Nature.

[11]  P. Wood.  Specificity in the interaction of direct dyes with polysaccharides , 1980 .

[12]  R. Kolter,et al.  Biofilm formation as microbial development. , 2000, Annual review of microbiology.

[13]  E. Seagren,et al.  Bacterial survival in biofilms: probes for exopolysaccharide and its hydrolysis, and measurements of intra- and interphase mass fluxes. , 1999, Methods in enzymology.

[14]  L. Carr,et al.  AN ASSESSMENT OF LIVEHAUL POULTRY TRANSPORT CONTAINER DECONTAMINATION , 1999 .

[15]  K. Jann,et al.  Structure and biosynthesis of the capsular antigens of Escherichia coli. , 1990, Current topics in microbiology and immunology.

[16]  R. Weiner,et al.  Polysaccharide exopolymer adhesives from periphytic marine bacteria , 1989 .