The influence of cellular source on periodontal regeneration using calcium phosphate coated polycaprolactone scaffold supported cell sheets.

Cell-based therapy is considered a promising approach to achieving predictable periodontal regeneration. In this study, the regenerative potential of cell sheets derived from different parts of the periodontium (gingival connective tissue, alveolar bone and periodontal ligament) were investigated in an athymic rat periodontal defect model. Periodontal ligament (PDLC), alveolar bone (ABC) and gingival margin-derived cells (GMC) were obtained from human donors. The osteogenic potential of the primary cultures was demonstrated in vitro. Cell sheets supported by a calcium phosphate coated melt electrospun polycaprolactone (CaP-PCL) scaffold were transplanted to denuded root surfaces in surgically created periodontal defects, and allowed to heal for 1 and 4 weeks. The CaP-PCL scaffold alone was able to promote alveolar bone formation within the defect after 4 weeks. The addition of ABC and PDLC sheets resulted in significant periodontal attachment formation. The GMC sheets did not promote periodontal regeneration on the root surface and inhibited bone formation within the CaP-PCL scaffold. In conclusion, the combination of either PDLC or ABC sheets with a CaP-PCL scaffold could promote periodontal regeneration, but ABC sheets were not as effective as PDLC sheets in promoting new attachment formation.

[1]  W. Young,et al.  Tissue engineering for bone regeneration using differentiated alveolar bone cells in collagen scaffolds. , 2003, Tissue engineering.

[2]  E. Putnins,et al.  Periodontal regeneration using engineered bone marrow mesenchymal stromal cells. , 2010, Biomaterials.

[3]  T. Okano,et al.  Periodontal ligament cell sheet promotes periodontal regeneration in athymic rats. , 2008, Journal of clinical periodontology.

[4]  Seong-Ho Choi,et al.  Isolation and characterization of human periodontal ligament (PDL) stem cells (PDLSCs) from the inflamed PDL tissue: in vitro and in vivo evaluations. , 2011, Journal of clinical periodontology.

[5]  S. Gronthos,et al.  Stem cells in the periodontal ligament. , 2006, Oral diseases.

[6]  Masayuki Yamato,et al.  Comparison of different tissue-derived stem cell sheets for periodontal regeneration in a canine 1-wall defect model. , 2011, Biomaterials.

[7]  J. Ingle,et al.  Bisphosphonates directly regulate cell proliferation, differentiation, and gene expression in human osteoblasts. , 2000, Cancer research.

[8]  Ying Zheng,et al.  Periodontal Ligament Stem Cell‐Mediated Treatment for Periodontitis in Miniature Swine , 2008, Stem cells.

[9]  S. Shi,et al.  Mesenchymal Stem Cells Derived from Human Gingiva Are Capable of Immunomodulatory Functions and Ameliorate Inflammation-Related Tissue Destruction in Experimental Colitis1 , 2009, The Journal of Immunology.

[10]  飛田 護邦.  Periodontal tissue regeneration with adipose-derived stem cells , 2010 .

[11]  Faming Chen,et al.  Stem cell-delivery therapeutics for periodontal tissue regeneration. , 2012, Biomaterials.

[12]  N. Takahashi,et al.  Fibroblastic cells derived from bovine periodontal ligaments have the phenotypes of osteoblasts. , 1990, Journal of periodontal research.

[13]  L. Bonassar,et al.  A novel approach to regenerating periodontal tissue by grafting autologous cultured periosteum. , 2006, Tissue engineering.

[14]  S. Gronthos,et al.  Stem cells and future periodontal regeneration. , 2009, Periodontology 2000.

[15]  S. Ivanovski.  Periodontal regeneration. , 2009, Australian dental journal.

[16]  T. Okano,et al.  Cell sheet engineering and other novel cell-based approaches to periodontal regeneration. , 2009, Periodontology 2000.

[17]  T. Karring,et al.  The regenerative potential of the periodontal ligament. An experimental study in the monkey. , 1982, Journal of clinical periodontology.

[18]  G. Tomar,et al.  Human gingiva-derived mesenchymal stem cells are superior to bone marrow-derived mesenchymal stem cells for cell therapy in regenerative medicine. , 2010, Biochemical and biophysical research communications.

[19]  S. Sälzer,et al.  Periodontal regeneration employing gingival margin-derived stem/progenitor cells: an animal study. , 2012, Journal of clinical periodontology.

[20]  Byung-Ho Choi,et al.  A comparative qualitative histological analysis of tissue-engineered bone using bone marrow mesenchymal stem cells, alveolar bone cells, and periosteal cells. , 2006, Oral surgery, oral medicine, oral pathology, oral radiology, and endodontics.

[21]  Masayuki Yamato,et al.  Human periodontal ligament cell sheets can regenerate periodontal ligament tissue in an athymic rat model. , 2005, Tissue engineering.

[22]  K. Mrozik,et al.  Isolation and characterization of mesenchymal stem cell-like cells from healthy and inflamed gingival tissue: potential use for clinical therapy. , 2012, Regenerative medicine.

[23]  C. Vaquette,et al.  Increasing electrospun scaffold pore size with tailored collectors for improved cell penetration. , 2011, Acta biomaterialia.

[24]  Saso Ivanovski,et al.  Effect of culture conditions and calcium phosphate coating on ectopic bone formation. , 2013, Biomaterials.

[25]  Stan Gronthos,et al.  Investigation of multipotent postnatal stem cells from human periodontal ligament , 2004, The Lancet.

[26]  F. Feng,et al.  Utility of PDL progenitors for in vivo tissue regeneration: a report of 3 cases. , 2010, Oral diseases.

[27]  B. Coulomb,et al.  Multipotent progenitor cells in gingival connective tissue. , 2010, Tissue engineering. Part A.

[28]  Wei Wang,et al.  Allogeneic Periodontal Ligament Stem Cell Therapy for Periodontitis in Swine , 2010, Stem cells.

[29]  Yan Jin,et al.  Heterogeneous dental follicle cells and the regeneration of complex periodontal tissues. , 2012, Tissue engineering. Part A.

[30]  S. Hsu,et al.  Enhanced chondrogenic differentiation potential of human gingival fibroblasts by spheroid formation on chitosan membranes. , 2012, Tissue engineering. Part A.

[31]  M. Somerman,et al.  Role of two mineral-associated adhesion molecules, osteopontin and bone sialoprotein, during cementogenesis. , 1995, Connective tissue research.

[32]  Nesrine Z. Mostafa,et al.  In Vitro Osteogenic Induction Of Human Gingival Fibroblasts For Bone Regeneration , 2010, The open dentistry journal.

[33]  Masayuki Yamato,et al.  Periodontal regeneration with multi-layered periodontal ligament-derived cell sheets in a canine model. , 2009, Biomaterials.

[34]  L. Tang,et al.  Characterization of mesenchymal stem cells from human normal and hyperplastic gingiva , 2011, Journal of cellular physiology.

[35]  J. Wennström,et al.  New attachment formation in the human periodontium by guided tissue regeneration. Case reports. , 1986, Journal of clinical periodontology.

[36]  S. Ivanovski,et al.  Isolation and characterization of fibroblasts derived from regenerating human periodontal defects. , 2001, Archives of oral biology.

[37]  C. Dörfer,et al.  Isolation and characterization of multipotent postnatal stem/progenitor cells from human alveolar bone proper. , 2012, Journal of cranio-maxillo-facial surgery : official publication of the European Association for Cranio-Maxillo-Facial Surgery.

[38]  Newell W Johnson,et al.  Periodontal diseases. , 2005, Lancet.

[39]  Wei Fan,et al.  A biphasic scaffold design combined with cell sheet technology for simultaneous regeneration of alveolar bone/periodontal ligament complex. , 2012, Biomaterials.

[40]  Y. Tabata,et al.  In situ tissue engineering of periodontal tissues by seeding with periodontal ligament-derived cells. , 2004, Tissue engineering.

[41]  V. Arana-Chavez,et al.  In vitro effects of ascorbic acid and β-glycerophosphate on human gingival fibroblast cells. , 2012, Tissue & cell.

[42]  M. Somerman,et al.  Human periodontal cells initiate mineral-like nodules in vitro. , 1991, Journal of periodontology.

[43]  T. Karring,et al.  New attachment following surgical treatment of human periodontal disease. , 1982, Journal of clinical periodontology.

[44]  M. Tanaka,et al.  Periodontal tissue engineering by transplantation of multilayered sheets of phenotypically modified gingival fibroblasts. , 2008, Journal of periodontal research.

[45]  M. Waters,et al.  Growth hormone regulates osteogenic marker mRNA expression in human periodontal fibroblasts and alveolar bone-derived cells. , 2003, Journal of periodontal research.

[46]  K. Omori,et al.  A tissue-engineered trachea derived from a framed collagen scaffold, gingival fibroblasts and adipose-derived stem cells. , 2010, Biomaterials.

[47]  S. Ivanovski,et al.  An immunohistochemical study of matrix molecules associated with barrier membrane-mediated periodontal wound healing. , 2000, Journal of periodontal research.

[48]  T. Okano,et al.  Assessment of cell sheets derived from human periodontal ligament cells: a pre-clinical study , 2010, Cell and Tissue Research.

[49]  W L Lin,et al.  Differentiation of periodontal ligament fibroblasts into osteoblasts during socket healing after tooth extraction in the rat , 1994, The Anatomical record.

[50]  G. R. Imm,et al.  A Comparative Study of Human Periodontal Ligament Cells and Gingival Fibroblasts in vitro , 1988, Journal of dental research.