Tuning nano electric field to affect restrictive membrane area on localized single cell nano-electroporation

Interaction of electric field with biological cells is an important phenomenon for field induced drug delivery system. We demonstrate a selective and localized single cell nano-electroporation (LSCNEP) by applying an intense electric field on a submicron region of the single cell membrane, which can effectively allow high efficient molecular delivery but low cell damage. The delivery rate is controlled by adjusting transmembrane potential and manipulating membrane status. Thermal and ionic influences are deteriorated from the cell membrane by dielectric passivation. Either reversible or irreversible by LSCNEP can fully controlled with potential applications in medical diagnostics and biological studies.

[1]  H P Schwan,et al.  Cellular membrane potentials induced by alternating fields. , 1992, Biophysical journal.

[2]  Dong-Chul Han,et al.  An impulsive, electropulsation-driven backflow in microchannels during electroporation. , 2008, Lab on a chip.

[3]  T. Tsong,et al.  Electroporation of cell membranes. , 1991, Biophysical journal.

[4]  Keunchang Cho,et al.  A novel electroporation method using a capillary and wire-type electrode. , 2008, Biosensors & bioelectronics.

[5]  Saeid Movahed,et al.  Microfluidics cell electroporation , 2011 .

[6]  U. Zimmermann,et al.  Electric field-mediated fusion and related electrical phenomena. , 1982, Biochimica et biophysica acta.

[7]  R. Schmid,et al.  Liposome mediated gene transfer into the rat oesophagus , 1997, Gut.

[8]  M. Davidson,et al.  Protein adsorption onto polystyrene surfaces studied by XPS and AFM , 2004 .

[9]  Fan-Gang Tseng,et al.  Delivery of molecules into cells using localized single cell electroporation on ITO micro-electrode based transparent chip , 2012, Biomedical microdevices.

[10]  Dong-Chul Han,et al.  Electrotransfection of mammalian cells using microchannel-type electroporation chip. , 2004, Analytical chemistry.

[11]  Rashid Bashir,et al.  Ultra-localized single cell electroporation using silicon nanowires. , 2013, Lab on a chip.

[12]  O Orwar,et al.  Altering the biochemical state of individual cultured cells and organelles with ultramicroelectrodes. , 1998, Proceedings of the National Academy of Sciences of the United States of America.

[13]  Dharmakeerthi Nawarathna,et al.  Localized electroporation and molecular delivery into single living cells by atomic force microscopy , 2008 .

[14]  E. Neumann,et al.  Gene transfer into mouse lyoma cells by electroporation in high electric fields. , 1982, The EMBO journal.

[15]  J Teissié,et al.  Direct observation in the millisecond time range of fluorescent molecule asymmetrical interaction with the electropermeabilized cell membrane. , 1997, Biophysical journal.

[16]  D. Miklavčič,et al.  Electroporation in dense cell suspension--theoretical and experimental analysis of ion diffusion and cell permeabilization. , 2007, Biochimica et biophysica acta.

[17]  J Teissié,et al.  Electropermeabilization of mammalian cells to macromolecules: control by pulse duration. , 1998, Biophysical journal.

[18]  E. Tekle,et al.  Electro-permeabilization of cell membranes: effect of the resting membrane potential. , 1990, Biochemical and biophysical research communications.

[19]  Yu-Cheng Lin,et al.  Electroporation microchips for in vitro gene transfection , 2001 .

[20]  Bo Yu,et al.  Nanochannel electroporation delivers precise amounts of biomolecules into living cells. , 2011, Nature nanotechnology.