Spatial-Temporal Codec Accuracy Calibration for Multi-scale Giga-Pixel Macroscope

The video-rate giga-pixel imaging macroscope with centimeter scale field of view and micron resolution, is an indispensable tool for the study of system biology. Its camera array based intrinsic nature brings a significant increase in throughput, but also challenges the applicability and accuracy of existing camera calibration methods. Here we proposed a spatial-temporal coded accuracy calibration method for multi-scale giga-pixel macroscope, which can achieve 0.199 pixels re-projection error. Meanwhile, a vignetting correction is also performed, for the seamless stitching under the condition of multi-camera brightness response anisotropy.

[1]  X. Xie,et al.  Nonperturbative chemical imaging of organelle transport in living cells with coherent anti-stokes Raman scattering microscopy. , 2006, Biophysical journal.

[2]  Jiakun Bao,et al.  An effective consistency correction and blending method for camera-array-based microscopy imaging , 2017, 2017 International Conference on Systems, Signals and Image Processing (IWSSIP).

[3]  Peter Jansen,et al.  Challenges in gigapixel multiscale image formation , 2012 .

[4]  Zhengyou Zhang,et al.  A Flexible New Technique for Camera Calibration , 2000, IEEE Trans. Pattern Anal. Mach. Intell..

[5]  Robert C. Bolles,et al.  Random sample consensus: a paradigm for model fitting with applications to image analysis and automated cartography , 1981, CACM.

[6]  T Meyer,et al.  Seamless stitching of tile scan microscope images , 2015, Journal of microscopy.

[7]  Paul R. Cohen,et al.  Camera Calibration with Distortion Models and Accuracy Evaluation , 1992, IEEE Trans. Pattern Anal. Mach. Intell..

[8]  M. Wand,et al.  Multivariate Locally Weighted Least Squares Regression , 1994 .

[9]  Roger Y. Tsai,et al.  A versatile camera calibration technique for high-accuracy 3D machine vision metrology using off-the-shelf TV cameras and lenses , 1987, IEEE J. Robotics Autom..

[10]  David J. Brady,et al.  Multiscale gigapixel photography , 2012, Nature.

[11]  Marc Levoy,et al.  Using plane + parallax for calibrating dense camera arrays , 2004, Proceedings of the 2004 IEEE Computer Society Conference on Computer Vision and Pattern Recognition, 2004. CVPR 2004..

[12]  Qionghai Dai,et al.  Multiscale gigapixel video: A cross resolution image matching and warping approach , 2017, 2017 IEEE International Conference on Computational Photography (ICCP).

[13]  Christian Matthäus,et al.  A compact microscope setup for multimodal nonlinear imaging in clinics and its application to disease diagnostics. , 2013, The Analyst.

[14]  S. Stigler.  Gauss and the Invention of Least Squares , 1981 .

[15]  D. G. Simpson,et al.  Introduction to Rousseeuw (1984) Least Median of Squares Regression , 1997 .

[16]  R. Horstmeyer,et al.  Wide-field, high-resolution Fourier ptychographic microscopy , 2013, Nature Photonics.

[17]  Fumiaki Tomita,et al.  Plane-based calibration algorithm for multi-camera systems via factorization of homography matrices , 2003, Proceedings Ninth IEEE International Conference on Computer Vision.