Lactate and pH change in close correlation in the extracellular space of the rat brain during cortical spreading depression

pH sensitive microcelectrodes were used in combination with microdialysis (MD) technique to measure extracellular pH (pHe) and extracellular lactate (lace) within the cortex of rat brains during cortical spreading depression (SD). SD was induced by local K(+)-application and identified by DC recordings. It was accompanied by an extracellular acidification of 0.34 +/- 0.06 pH units and by a 2.8 +/- 0.80 fold increase of lace; the recovery of pHe took place within three phases, that of lace within 2 phases. The recovery of both parameters was complete about 45 min after the onset of SD. We conclude that the changes of lace and pHe are closely related. This indicates both lactate and protons to be transported in parallel.

[1]  L. Symon,et al.  A rapid redistribution of hydrogen ions is associated with depolarization and repolarization subsequent to cerebral ischemia reperfusion. , 1990, Journal of neurophysiology.

[2]  A. Hansen,et al.  Effect of anoxia on ion distribution in the brain. , 1985, Physiological reviews.

[3]  S. Mukerji,et al.  Simulation of aspects of ischemia in cell culture: Changes in lactate compartmentation , 1990, Glia.

[4]  R D Piper,et al.  Cortical blood flow changes during spreading depression in cats. , 1991, The American journal of physiology.

[5]  W. Paschen,et al.  Regional changes of blood flow, glucose, and ATP content determined on brain sections during a single passage of spreading depression in rat brain cortex , 1984, Experimental Neurology.

[6]  S. Mukerji,et al.  Lactate release from cultured astrocytes and neurons: A comparison , 1988, Glia.

[7]  J. Lear,et al.  Autoradiographic Measurement of Cerebral Lactate Transport Rate Constants in Normal and Activated Conditions , 1991, Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism.

[8]  C. Nicholson,et al.  Alkaline and acid transients in cerebellar microenvironment. , 1983, Journal of neurophysiology.

[9]  J. Křivánek.  SOME METABOLIC CHANGES ACCOMPANYING LEÃO'S SPREADING CORTICAL DEPRESSION IN THE RAT , 1961, Journal of neurochemistry.

[10]  W. Schlue,et al.  The regulation of intracellular pH by identified glial cells and neurones in the central nervous system of the leech. , 1987, The Journal of physiology.

[11]  M. Lauritzen,et al.  Headache Persistent oligemia of rat cerebral cortex in the wake of spreading depression Ann. Neurol., 12 (1982) 469–474 , 1983, Pain.

[12]  O. Paulson,et al.  Journal of Cerebral Blood Flow and Metabolism Kinetic Analysis of the Human Blood-brain Barrier Transport of Lactate and Its Influence by Hypercapnia Materials and Methods Patients Blood-brain Barrier Permeability Measurements , 2022 .

[13]  C. Nicholson,et al.  Extracellular ionic variations during spreading depression , 1978, Neuroscience.

[14]  M. H. Jacobs.  SOME ASPECTS OF CELL PERMEABILITY TO WEAK ELECTROLYTES , 1940 .

[15]  W. Paschen,et al.  Lactate and pH in the Brain: Association and Dissociation in Different Pathophysiological States , 1987, Journal of neurochemistry.

[16]  A. Hansen,et al.  Extracellular pH Changes during Spreading Depression and Cerebral Ischemia: Mechanisms of Brain pH Regulation , 1984, Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism.

[17]  M. Lauritzen,et al.  Cerebral blood flow in migraine and cortical spreading depression. , 1987, Acta neurologica Scandinavica. Supplementum.

[18]  M. Lauritzen,et al.  Cortical Spreading Depression is Associated with Arachidonic Acid Accumulation and Preservation of Energy Charge , 1990, Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism.

[19]  J. Korf,et al.  In vivo Identification and Quantitative Evaluation of Carrier-Mediated Transport of Lactate at the Cellular Level in the Striatum of Conscious, Freely Moving Rats , 1988, Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism.

[20]  B. Siesjö,et al.  Brain energy metabolism , 1978 .

[21]  C. Nicholson,et al.  Anion permeability in spreading depression investigated with ion-sensitive microelectrodes , 1979, Brain Research.

[22]  H. Weiss,et al.  Cerebral Blood Flow and Oxygen Consumption in Cortical Spreading Depression , 1991, Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism.

[23]  D. Scheller,et al.  The internal reference technique in microdialysis: A practical approach to monitoring dialysis efficiency and to calculating tissue concentration from dialysate samples , 1991, Journal of Neuroscience Methods.

[24]  Albert Gjedde,et al.  Persistent oligemia of rat cerebral cortex in the wake of spreading depression , 1982, Annals of neurology.

[25]  O. Burešová,et al.  The mechanism and applications of Leão's spreading depression of electroencephalographic activity , 1974 .

[26]  A. A. Leão,et al.  SPREADING DEPRESSION OF ACTIVITY IN THE CEREBRAL CORTEX , 1944 .