Beyond filtered backprojection: A reconstruction software package for ion beam microtomography data

A new version of the TomoRebuild data reduction software package is presented, for the reconstruction of scanning transmission ion microscopy tomography (STIMT) and particle induced X-ray emission tomography (PIXET) images. First, we present a state of the art of the reconstruction codes available for ion beam microtomography. The algorithm proposed here brings several advantages. It is a portable, multi-platform code, designed in C++ with well-separated classes for easier use and evolution. Data reduction is separated in different steps and the intermediate results may be checked if necessary. Although no additional graphic library or numerical tool is required to run the program as a command line, a user friendly interface was designed in Java, as an ImageJ plugin. All experimental and reconstruction parameters may be entered either through this plugin or directly in text format files. A simple standard format is proposed for the input of experimental data. Optional graphic applications using the ROOT interface may be used separately to display and fit energy spectra. Regarding the reconstruction process, the filtered backprojection (FBP) algorithm, already present in the previous version of the code, was optimized so that it is about 10 times as fast. In addition, Maximum Likelihood Expectation Maximization (MLEM) and its accelerated version Ordered Subsets Expectation Maximization (OSEM) algorithms were implemented. A detailed user guide in English is available. A reconstruction example of experimental data from a biological sample is given. It shows the capability of the code to reduce noise in the sinograms and to deal with incomplete data, which puts a new perspective on tomography using low number of projections or limited angle.

[1]  Tilman Butz,et al.  Limited angle STIM and PIXE tomography of single cells , 2010 .

[2]  Tilo Reinert,et al.  First results on ion micro-tomography at LIPSION , 2010 .

[3]  P. Barberet,et al.  Technical developments for computed tomography on the CENBG nanobeam line , 2011 .

[4]  H. Seznec,et al.  Reconstruction of 3D ion beam micro-tomography data for applications in Cell Biology , 2009 .

[5]  I. D. Proctor,et al.  Ion microbeam tomography , 1989 .

[6]  Arlyn J. Antolak,et al.  PIXE tomography of samples with inhomogeneous elemental composition , 1994 .

[7]  V. Lučić,et al.  Cryo‐electron tomography: methodology, developments and biological applications , 2011, Journal of microscopy.

[8]  R. W. Smith,et al.  TomoRebuild: a new data reduction software package for scanning transmission ion microscopy tomography , 2005 .

[9]  Nicholas M. Spyrou,et al.  3D quantitative elemental mapping using simultaneous proton induced X-ray emission tomography and scanning transmission ion microscopy tomography , 2007 .

[10]  李幼升,et al.  Ph , 1989 .

[11]  George K. Loudos An efficient analytical calculation of probability matrix in 2D SPECT , 2008, Comput. Medical Imaging Graph..

[12]  H. W. Lefevre,et al.  PIXE-STIM microtomography: Zinc and manganese concentrations in a scorpion stinger , 1992 .

[13]  A. Antolak,et al.  Some practical considerations for ion microtomography , 1990 .

[14]  B. Fischer,et al.  Microtomography by heavy ions , 1990 .

[15]  Grant T. Gullberg,et al.  Users Manual: Donner Algorithms for Reconstruction Tomography , 1977 .

[16]  T. Andrea,et al.  The improved STIM tomography set-up at LIPSION: Three-dimensional reconstruction of biological samples , 2009 .

[18]  Ph. Moretto,et al.  3D imaging of microscopic structures using a proton beam , 2005, IEEE Transactions on Nuclear Science.

[19]  B. L. Henke,et al.  X-Ray Interactions: Photoabsorption, Scattering, Transmission, and Reflection at E = 50-30,000 eV, Z = 1-92 , 1993 .

[20]  D. T. Nguyen Développement d'algorithmes de reconstruction tomographique pour l'analyse PIXE d'échantillons biologiques , 2008 .

[21]  Soo Chin Liew,et al.  A PIXE micro-tomography experiment using MLEM algorithm , 1997 .

[22]  Keith A. Nugent,et al.  Submicron STIM tomography reconstruction techniques , 1991 .

[23]  H. Malcolm Hudson,et al.  Accelerated image reconstruction using ordered subsets of projection data , 1994, IEEE Trans. Medical Imaging.

[24]  G.J.F. Legge,et al.  An accurate reconstruction algorithm for tomography experiments that involve complex probe - sample interactions , 1997 .