Detection of drug‐responsive B lymphocytes and antidrug IgG in patients with β‐lactam hypersensitivity

Delayed‐type β‐lactam hypersensitivity develops in subset of patients. The cellular immunological processes that underlie the drug‐specific response have been described; however, little is known about involvement of the humoral immune system. Thus, the aim of this study was to utilize piperacillin hypersensitivity as an exemplar to (i) develop cell culture methods for the detection of drug‐specific B‐cell responses, (ii) characterize drug‐specific IgG subtypes and (iii) assess reactivity of IgG antibodies against proteins modified to different levels with piperacillin haptens.

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