COMPARATIVE EFFICIENCY IN DIFFERENT METHODS OF EXTRACTING NEMATODE FROM SOIL AND PLANT MATERIAL

Nematodes cause lot of damage to crops, in order to identify and control them the proper quantification of its population in the soiland/or plant should be known through the use of different extraction methods. These extraction methods were used and compared for suitability in terms of the life stages of nematodes extracted from soil and plant material. Seinhorst method is found to be t he most appropriate technique for extraction of cyst nematode, while the Automatic Zonal Centrifugation was the most suitable in extracting mostly all stages of the nematodes from both soil and plant materials. Different techniques have its own advantages and disadvantages in which the choice of the desired technique depends mainly on the target stage of the nematode that an individual will be working with or to the purpose of extraction.