The nonstructural component of the Abelson murine leukemia virus polyprotein P120 is encoded by newly acquired genetic sequences

The Abelson leukemia virus (AbLV) polyprotein P120 is compared to translational products representing the entire Moloney murine leukemia virus (MuLV) genome on the basis of [35S]methionine tryptic peptide composition. Three methionine-containing tryptic peptides present in Moloney Pr65gag are each shown to be present in both Pr75gag and in Pr180gag-pol. Of these, one peptide, corresponding to Moloney MuLV p12, but neither of two p30-specific peptides are present in AbLV P120. Among the 12 remaining methionine-containing peptides present in AbLV P120, many, if not all, are unique to AbLV P120 and not shared by either Moloney MuLV Pr180gag-pol or Pr82gag.

[1]  J. Stephenson,et al.  Abelson murine leukemia virus-infected cell lines defective in transformation. , 1979, Virology.

[2]  J. Kopchick,et al.  Tryptic peptide analysis of gag and gag-pol gene products of Rauscher murine leukemia virus , 1979, Journal of virology.

[3]  R. K. Reynolds,et al.  Translation of type C viral RNAs in Xenopus laevis oocytes: evidence that the 120,000-molecular-weight polyprotein expressed in Abelson leukemia virus-transformed cells is virus coded , 1978, Journal of virology.

[4]  H. Varmus,et al.  Evidence that the transforming gene of avian sarcoma virus encodes a protein kinase associated with a phosphoprotein , 1978, Cell.

[5]  J. Stephenson,et al.  Murine leukemia virus (T-8)-transformed cells: identification of a precursor polyprotein containing gag gene-coded proteins (p15 and p12) and a nonstructural component , 1978, Journal of virology.

[6]  J. Stephenson,et al.  Cells nonproductively transformed by Abelson murine leukemia virus express a high molecular weight polyprotein containing structural and nonstructural components. , 1978, Proceedings of the National Academy of Sciences of the United States of America.

[7]  D. Baltimore,et al.  Identification of an Abelson murine leukemia virus-encoded protein present in transformed fibroblast and lymphoid cells. , 1978, Proceedings of the National Academy of Sciences of the United States of America.

[8]  R. Erikson,et al.  Protein kinase activity associated with the avian sarcoma virus src gene product. , 1978, Proceedings of the National Academy of Sciences of the United States of America.

[9]  R. Weinberg,et al.  Translation of MuLV and MSV RNAs in nuclease-treated reticulocyte extracts: Enhancement of the gag-pol polypeptide with yeast suppressor tRNA , 1978, Cell.

[10]  D. Baltimore,et al.  In vitro transformation of lymphoid cells by Abelson murine leukemia virus. , 1975, Proceedings of the National Academy of Sciences of the United States of America.

[11]  W. Bonner,et al.  A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels. , 1974, European journal of biochemistry.

[12]  U. K. Laemmli,et al.  Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4 , 1970, Nature.

[13]  H. Abelson,et al.  Lymphosarcoma: virus-induced thymic-independent disease in mice. , 1970, Cancer research.