Hyperbaric oxygenation on adhesions prevention after laparotomy in rats.

PURPOSE To evaluate the effects of hyperbaric oxygenation on prevention of adhesions in the abdominal cavity after laparotomy. METHODS Fifty four rats underwent laparotomy; stitches were made in the four quadrant parietal peritoneum and abdominal cavity closure. Animals were divided into three groups: 1 - control; 2 - subjected to high pressures and oxygenation; 3 - subjected to 100% hyperbaric oxygenation. The animals in groups 2 and 3 were daily submitted to oxygenation hyperbaric chamber after surgery. On the seventh day another laparotomy, registration of procedure, assessment of adhesions and biopsies of the peritoneum were held. Professionals analyzed the videos and the biopsies. RESULTS Peritoneal cavity adhesions occurred in animals of three groups with no difference between them. In Group 3, the adhesions presented more fragile and vascular proliferation more pronounced, and there was no difference in comparison with the first and second groups. However, there was no significant difference in the evaluation of these parameters between the animals in groups 1 and 2. CONCLUSIONS Postoperative hyperbaric oxygenation in rats submitted to laparotomy did not alter the frequency, but reduced the density of adhesions in the peritoneal cavity and promoted vascular proliferation. The change in atmospheric pressure alone had no influence on the results.

[1]  L. Nair.  Hyperbaric Medicine. , 2015, Connecticut medicine.

[2]  T. Kooistra,et al.  Pathogenesis of postoperative adhesion formation , 2011, The British journal of surgery.

[3]  B. Miedema,et al.  In search of the best peritoneal adhesion model: comparison of different techniques in a rat model. , 2011, The Journal of surgical research.

[4]  R. Schonman,et al.  Postoperative inflammation in the abdominal cavity increases adhesion formation in a laparoscopic mouse model. , 2011, Fertility and sterility.

[5]  K. Inaba,et al.  Prevention of postoperative peritoneal adhesions: a review of the literature. , 2011, American journal of surgery.

[6]  A. Barbul,et al.  Intra-abdominal healing: gastrointestinal tract and adhesions. , 2010, The Surgical clinics of North America.

[7]  G. Maddern,et al.  Abdominal Adhesion Prevention: Still a Sticky Subject , 2010, Digestive Surgery.

[8]  R. M. Kamel.  Prevention of postoperative peritoneal adhesions. , 2010, European journal of obstetrics, gynecology, and reproductive biology.

[9]  T. Ozgurtas,et al.  Synergistic effects of hyperbaric oxygen and granulocytecolony stimulating factor on postoperative adhesion formation in a rat cecal abrasion model. , 2010, Bratislavske lekarske listy.

[10]  G. Leung,et al.  Adjuvant therapy for the reduction of postoperative intra-abdominal adhesion formation. , 2009, Asian journal of surgery.

[11]  Ö. Ekinci,et al.  Reduction of Postsurgical Adhesions in a Rat Model: A Comparative Study , 2009, Clinics.

[12]  G. Saed,et al.  Postoperative Adhesions: From Formation to Prevention , 2008, Seminars in reproductive medicine.

[13]  E. Gouvêa,et al.  Comparative study between parietal peritoneum suture and nonsuture in midline laparotomies in rats. , 2008, Acta cirurgica brasileira.

[14]  Marcelo Corrales,et al.  Preventing intraperitoneal adhesions with vitamin E and sodium hyaluronate/carboxymethylcellulose: a comparative study in rats. , 2008, Acta cirurgica brasileira.

[15]  J. Bazin,et al.  Effects of supplemental perioperative oxygen on post-operative abdominal wound adhesions in a mouse laparotomy model with controlled respiratory support. , 2007, Human reproduction.

[16]  Zacarias Alves de Souza Filho,et al.  Abdominal wall healing in reoperated rats. , 2007, Acta cirurgica brasileira.

[17]  A. Diogo Filho,et al.  [Peritoneostomy with latex coated polypropylene: experimental study in rats]. , 2006, Acta cirurgica brasileira.

[18]  N. Al-Waili,et al.  Effects of Hyperbaric Oxygen on Inflammatory Response to Wound and Trauma: Possible Mechanism of Action , 2006, TheScientificWorldJournal.

[19]  T. Ozgurtas,et al.  Preoperative and Postoperative Administration of Hyperbaric Oxygen Improves Biochemical and Mechanical Parameters on Ischemic and Normal Colonic Anastomoses , 2006, Journal of investigative surgery : the official journal of the Academy of Surgical Research.

[20]  T. A. Nunes,et al.  [Hyperbaric oxygen therapy in rats submitted to hepatic veins ligation: mortality valuation and histological study of liver and spleen]. , 2006, Acta cirurgica brasileira.

[21]  U. Kayisli,et al.  Inhibition of chemokines prevents intraperitoneal adhesions in mice , 2005 .

[22]  H. Ellis.  Intraabdominal and postoperative peritoneal adhesions. , 2005, Journal of the American College of Surgeons.

[23]  S. Bozkurt,et al.  Intraperitoneal Tenoxicam to Prevent Abdominal Adhesion Formation in a Rat Peritonitis Model , 2005, Surgery Today.

[24]  J. Becker,et al.  Intra-abdominal adhesion prevention: are we getting any closer? , 2004, Annals of surgery.

[25]  Finn Gottrup,et al.  Oxygen in Wound Healing and Infection , 2004, World journal of surgery.

[26]  P. Koninckx,et al.  Reactive oxygen species and adhesion formation: clinical implications in adhesion prevention. , 2003, Human reproduction.

[27]  W L Ledger,et al.  Peritoneal healing and adhesion formation/reformation. , 2001, Human reproduction update.

[28]  A. Johns.  Evidence-based prevention of post-operative adhesions. , 2001, Human reproduction update.

[29]  P. Novak,et al.  Peritoneal mesothelial hypoxia during pneumoperitoneum is a cofactor in adhesion formation in a laparoscopic mouse model. , 2001, Fertility and sterility.

[30]  M. Biondo-Simões,et al.  Effect of streptokinase in the prevention of intra-abdominal adhesions in the rat , 1997 .

[31]  C. Ohmann,et al.  Preventing recurrent postoperative adhesions: an experimental study in rats. , 1989, European surgical research. Europaische chirurgische Forschung. Recherches chirurgicales europeennes.

[32]  E. Wallach,et al.  Prevention and management of peritoneal adhesions. , 1984, Fertility and sterility.

[33]  K. Kalambaheti,et al.  PREVENTION OF ADHESIONS WITH POLYVINYLPYRROLIDONE. PRELIMINARY REPORT. , 1964, Archives of surgery.