Effects of pH and phosphate on the production of struvite by Myxococcus xanthus

We studied the influence of pH and the phosphate content of the culture medium on the precipitation of struvite by Myxococcus xanthus, a bacterium that undergoes autolysis at the end of its exponential growth phase in liquid cultures. The best results were obtained with pH values between 7.2 and 8.0 and with a phosphate concentration of 10 mM. Our studies reveal for the first time that the precipitation of struvite always begins at the onset of autolysis and that culture conditions favoring the early occurrence of autolysis also enhance struvite production.

[1]  E. Montoya,et al.  Struvite production by Myxococcus coralloides D , 1993 .

[2]  B. Nelson,et al.  In vitro production of struvite by Bacillus pumilus , 1991 .

[3]  J. Nickel,et al.  Influence of chondroitin sulfate, heparin sulfate, and citrate on Proteus mirabilis-induced struvite crystallization in vitro. , 1990, The Journal of urology.

[4]  T. Beveridge,et al.  Metal Ions and Bacteria , 1989 .

[5]  Stephen Mann,et al.  Molecular recognition in biomineralization , 1988, Nature.

[6]  H. Hedelin,et al.  Urease-induced crystallization in synthetic urine. , 1985, The Journal of urology.

[7]  J. Brorson,et al.  Ureaplasma urealyticum-induced crystallization of magnesium ammonium phosphate and calcium phosphates in synthetic urine. , 1984, The Journal of urology.

[8]  J. Fowler Bacteriology of branched renal calculi and accompanying urinary tract infection. , 1984, The Journal of urology.

[9]  E. Montoya,et al.  Autolysis in Myxococcus coralloides D , 1983 .

[10]  R. Murray,et al.  Diagenesis of Metals Chemically Complexed to Bacteria: Laboratory Formation of Metal Phosphates, Sulfides, and Organic Condensates in Artificial Sediments , 1983, Applied and environmental microbiology.

[11]  H. Lowenstam,et al.  Minerals formed by organisms. , 1981, Science.

[12]  M. Dworkin,et al.  Morphogenesis and developmental interactions in myxobacteria. , 1975, Science.

[13]  E. J. Westbury Some observations on the quantitative analysis of over 1000 urinary calculi. , 1974, British journal of urology.

[14]  T. Stamey,et al.  Surgical, bacteriological, and biochemical management of "infection stones". , 1971, JAMA.

[15]  M. Mccallum,et al.  The precipitation of calcium carbonate from seawater by bacteria isolated from Bahama bank sediments. , 1970, The Journal of applied bacteriology.

[16]  N. Heatley,et al.  The occurrence of struvite (magnesium ammonium phosphate hexahydrate) in microbial cultures. , 1963, Journal of general microbiology.

[17]  I. F. Huddleson,et al.  Magnesium Ammonium Phosphate Crystals in Aerobic Cultures of Brucella Abortus and Brucella Melitensis , 1927 .

[18]  M. Rivadeneyra,et al.  Struvite formation by and : the influence of agitation , 1990 .

[19]  M. Rivadeneyra,et al.  The influence of pH on struvite formation by bacteria , 1989 .

[20]  T J Beveridge,et al.  Role of cellular design in bacterial metal accumulation and mineralization. , 1989, Annual review of microbiology.

[21]  M. Rivadeneyra,et al.  Influence of magnesium source and magnesium and phosphate concentration on bacterial struvite formation , 1989 .

[22]  J. Nickel,et al.  The ecology and pathogenicity of urease-producing bacteria in the urinary tract. , 1988, Critical reviews in microbiology.

[23]  S. Mann The study of biominerals by high resolution transmission electron microscopy. , 1986, Scanning electron microscopy.

[24]  Y. Berland,et al.  [Areas of magnesium ammonium phosphate nucleation in sterile, alkaline or acid urine]. , 1984, Nephrologie.

[25]  S. Weiner,et al.  Mineralization by Organisms and the Evolution of Biomineralization , 1983 .

[26]  L. Margulis,et al.  Microbial Systematics and a Gaian View of the Sediments , 1983 .

[27]  M. Rivadeneyra,et al.  Bacterial formation of struvite , 1983 .

[28]  S. Mann Mineralization in biological systems , 1983 .

[29]  H. Shinano,et al.  Effect of Magnesium Ion Concentration on the Types of Crystals Deposited by Marine Bacteria in Sea Water , 1975 .