Evaluation of Antioxidant Activity of Natural and Synthetic Compounds Against LDL Oxidation Using CNT Electrode

In this paper, a novel method for evaluating antioxidant activity against low density lipoprotein (LDL) oxidation (anti-LDLox) using a carbon nanotube (CNT) based electrode was investigated. Although ORAC and DPPH are known methods to evaluate antioxidant activity, they do not directly reflect the capacities of anti-LDLox. A method has been reported for measuring oxidized LDL using a CNT electrode. We evaluated anti-LDLox by measuring the amount of oxidized LDL using the electrode after incubation of LDL with antioxidants during copper-mediated oxidation. Thiobarbituric acid reactive substances (TBARS) were also determined for comparison with the CNT electrode. There was a correlation of the CNT electrode with the TBARS and the ORAC, whereas no correlation was found between the CNT electrode and the DPPH. We demonstrate here that a CNT electrode method has a potential for the determination of the antioxidant activity of various natural and synthetic compounds by detecting oxidized LDL.

[1]  R. Stocker,et al.  Vitamin E in human low-density lipoprotein. When and how this antioxidant becomes a pro-oxidant. , 1992, The Biochemical journal.

[2]  B. D. Malhotra,et al.  Polyaniline-carbon nanotube composite film for cholesterol biosensor. , 2008, Analytical biochemistry.

[3]  L. Cisneros-Zevallos,et al.  Comparison of ABTS, DPPH, FRAP, and ORAC assays for estimating antioxidant activity from guava fruit extracts , 2006 .

[4]  T. Sakurai,et al.  Quantitative determination of phosphatidylcholine hydroperoxides during copper oxidation of LDL and HDL by liquid chromatography/mass spectrometry , 2012, Analytical and Bioanalytical Chemistry.

[5]  Yuji Takahashi,et al.  Novel monoclonal antibody recognizing triglyceride-rich oxidized LDLs associated with severe liver disease and small oxidized LDLs in normal subjects , 2012, Annals of clinical biochemistry.

[6]  大川 博,et al.  Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction , 1979 .

[7]  A. Tuantranont,et al.  Fast cholesterol detection using flow injection microfluidic device with functionalized carbon nanotubes based electrochemical sensor. , 2010, Biosensors & bioelectronics.

[8]  J. Coombes,et al.  Astaxanthin: A Potential Therapeutic Agent in Cardiovascular Disease , 2011, Marine drugs.

[9]  H. Chiba,et al.  Improved HPLC assay for lipid peroxides in human plasma using the internal standard of hydroperoxide , 2005, Lipids.

[10]  J. Blumberg,et al.  Extraction methods determine the antioxidant capacity and induction of quinone reductase by soy products in vitro. , 2009, Food chemistry.

[11]  T. Sakurai,et al.  Detection of oxidized LDL using a carbon nanotube electrode , 2012 .

[12]  Koichi Shimizu,et al.  Fraction estimation of small, dense LDL using autocorrelation function of dynamic light scattering. , 2010, Optics express.

[13]  S. Yous,et al.  Antioxidant activity of benzoxazolinonic and benzothiazolinonic derivatives in the LDL oxidation model. , 2009, Bioorganic & medicinal chemistry.

[14]  G. Mazza,et al.  Assessing antioxidant and prooxidant activities of phenolic compounds. , 2000, Journal of agricultural and food chemistry.

[15]  J L Witztum,et al.  Beyond cholesterol. Modifications of low-density lipoprotein that increase its atherogenicity. , 1989, The New England journal of medicine.

[16]  G. Bellomo,et al.  When and why a water-soluble antioxidant becomes pro-oxidant during copper-induced low-density lipoprotein oxidation: a study using uric acid. , 1999, The Biochemical journal.

[17]  J. F. Stevens,et al.  Vitamins C and E: beneficial effects from a mechanistic perspective. , 2011, Free radical biology & medicine.

[18]  T. Sakurai,et al.  Evaluation of various electrode materials for detection of oxidized low-density lipoproteins. , 2013, Journal of biomedical nanotechnology.

[19]  Sandro Carrara,et al.  Screen-printed electrodes based on carbon nanotubes and cytochrome P450scc for highly sensitive cholesterol biosensors. , 2008, Biosensors & bioelectronics.

[20]  H. Alessio,et al.  Oxygen-radical absorbance capacity assay for antioxidants. , 1993, Free radical biology & medicine.

[21]  T. Osawa,et al.  Immunochemical detection of carboxymethylated Apo B-100 in copper-oxidized LDL. , 1996, Biochemical and biophysical research communications.

[22]  G. Desideri,et al.  Flavonoids: Antioxidants Against Atherosclerosis , 2010, Nutrients.

[23]  Tetsuya Seki,et al.  Serotonin derivatives, major safflower (Carthamus tinctorius L.) seed antioxidants, inhibit low-density lipoprotein (LDL) oxidation and atherosclerosis in apolipoprotein E-deficient mice. , 2006, Journal of agricultural and food chemistry.

[24]  N. Porter.  Mechanisms for the autoxidation of polyunsaturated lipids , 1986 .