Efficient immobilisation of industrial biocatalysts: criteria and constraints for the selection of organic polymeric carriers and immobilisation methods.

Efficient immobilisation protocols are the result of perfect matching of factors depending on the enzyme, the process and the support for immobilisation. Physical-chemical phenomena, such as partition, solvation and diffusion, strongly affect the efficiency of the biocatalyst in each specific reaction system. Therefore, tailored solutions must be developed for each specific process of interest. Indeed, direct investigation of what occurs at the molecular level in a reaction catalysed by an immobilised enzyme is a quite formidable task and observed differences in the performance of immobilised biocatalysts must be interpreted very carefully. In any study dealing with enzyme immobilisation the prerequisite is the rigorous planning and reporting of experiments, being aware of the complexity of these multi-phase systems.

[1]  Lucia Gardossi,et al.  Understanding enzyme immobilisation. , 2009, Chemical Society reviews.

[2]  L. Betancor,et al.  Bioinspired enzyme encapsulation for biocatalysis. , 2008, Trends in biotechnology.

[3]  A. Pierre,et al.  The sol-gel encapsulation of enzymes , 2004 .

[4]  Torben Vedel Borchert,et al.  Industrial enzyme applications. , 2002, Current opinion in biotechnology.

[5]  Ole Kirk,et al.  Industrial lipase immobilization , 2003 .

[6]  A. Lali,et al.  Immobilization of lipases for non-aqueous synthesis , 2006 .

[7]  Ephraim Katchalski-Katzir,et al.  Eupergit® C, a carrier for immobilization of enzymes of industrial potential , 2000 .

[8]  R. Sheldon.  Characteristic features and biotechnological applications of cross-linked enzyme aggregates (CLEAs) , 2011, Applied Microbiology and Biotechnology.

[9]  Silica-immobilized His6-tagged enzyme: alanine racemase in hydrophobic solvent. , 2008, Biotechnology and bioengineering.

[10]  O. Thum,et al.  Enzyme Stabilization by Deposition of Silicone Coatings , 2009 .

[11]  P. Halling,et al.  Proteomic methods applied to the analysis of immobilized biocatalysts , 2006, Biotechnology and bioengineering.

[12]  L. Goldstein.  Microenvironmental effects on enzyme catalysis. A kinetic study of polyanionic and polycationic derivatives of chymotrypsin. , 1972, Biochemistry.

[13]  J. Tramper,et al.  A multicomponent reaction–diffusion model of a heterogeneously distributed immobilized enzyme , 2006, Applied Microbiology and Biotechnology.

[14]  Vincenza Calabrò,et al.  Endo‐ and exo‐inulinases: Enzyme‐substrate interaction and rational immobilization , 2009, Biotechnology progress.

[15]  C. Ebert,et al.  Activity of covalently immobilised PGA in water miscible solvents at controlled aw , 2001 .

[16]  J. Porath.  Immobilized metal ion affinity chromatography. , 1992, Protein expression and purification.

[17]  A. Salis,et al.  Characterisation of Accurel MP1004 polypropylene powder and its use as a support for lipase immobilisation , 2003 .

[18]  Alessandra Basso,et al.  Computational methods to rationalize experimental strategies in biocatalysis. , 2006, Trends in biotechnology.

[19]  Roger A. Sheldon,et al.  Enzyme Immobilization: The Quest for Optimum Performance , 2007 .

[20]  V. Báles,et al.  Biochemical engineering of biocatalysts immobilized on cellulosic materials. , 1993, Enzyme and microbial technology.

[21]  G. Antranikian,et al.  Practical application of different enzymes immobilized on sepabeads , 2008, Bioprocess and biosystems engineering.

[22]  O. Abián,et al.  Epoxy-amino groups: a new tool for improved immobilization of proteins by the epoxy method. , 2003, Biomacromolecules.

[23]  C. Ebert,et al.  High isolated yields in thermodynamically controlled peptide synthesis in toluene catalysed by thermolysin adsorbed on Celite R-640 , 2000 .

[24]  S. Charaniya,et al.  A model system for increasing the intensity of whole‐cell biocatalysis: Investigation of the rate of oxidation of D‐sorbitol to L‐sorbose by thin bi‐layer latex coatings of non‐growing Gluconobacter oxydans , 2006, Biotechnology and bioengineering.

[25]  W. Tischer,et al.  Immobilized enzymes: crystals or carriers? , 1999, Trends in biotechnology.

[26]  R. Sheldon,et al.  Immobilization of Penicillin G Acylase: The Key to Optimum Performance , 2005 .

[27]  Owen P. Ward,et al.  Whole cell biocatalysis in nonconventional media , 1993, Journal of Industrial Microbiology.

[28]  T. Tosa,et al.  Studies on continuous enzyme reactions. I. Screening of carriers for preparation of water-insoluble aminoacylase. , 1966, Enzymologia.

[29]  Lucia Gardossi,et al.  Guidelines for reporting of biocatalytic reactions. , 2010, Trends in biotechnology.

[30]  I. Takata,et al.  Screening of matrix suitable for immobilization of microbial cells , 1977 .

[31]  V. Ferrario,et al.  Conformational Changes of Lipases in Aqueous Media: A Comparative Computational Study and Experimental Implications , 2011 .

[32]  P. Halling,et al.  Thermodynamic predictions for biocatalysis in nonconventional media: theory, tests, and recommendations for experimental design and analysis. , 1994, Enzyme and microbial technology.

[33]  S. Karboune,et al.  Immobilization of epoxide hydrolase from Aspergillus niger onto DEAE-cellulose: enzymatic properties and application for the enantioselective resolution of a racemic epoxide , 2005 .

[34]  Ole Kirk,et al.  Lipases from Candida antarctica: Unique Biocatalysts from a Unique Origin , 2002 .

[35]  Abdul Hameed,et al.  Industrial applications of microbial lipases , 2006 .

[36]  M. Alzohairy,et al.  Recent Advances and Applications of Immobilized Enzyme Technologies: A Review , 2010 .

[37]  R. Fernández-Lafuente,et al.  Additional stabilization of penicillin G acylase-agarose derivatives by controlled chemical modification with formaldehyde. , 1992, Enzyme and microbial technology.

[38]  Alessandra Basso,et al.  In Silico Analysis of Enzyme Surface and Glycosylation Effect as a Tool for Efficient Covalent Immobilisation of CalB and PGA on Sepabeads , 2007 .

[39]  Ying Mei,et al.  Imaging the distribution and secondary structure of immobilized enzymes using infrared microspectroscopy. , 2003, Biomacromolecules.

[40]  V. Nagarajan,et al.  Biocatalysis: applications and potentials for the chemical industry. , 2002, Trends in biotechnology.

[41]  Fructose Production by Inulinase Covalently Immobilized on Sepabeads in Batch and Fluidized Bed Bioreactor , 2010, International journal of molecular sciences.