Droplet movement control using fuzzy logic controller and image processing along with pin valves inside microfluidic device

Most of the research studies nowadays are trying to bring automation to biomedical engineering and Lab on a Chip which is fast growing interdisciplinary field and has attracted researchers from various fields. The objective of this paper is to present an overall system to control droplet movement inside microfluidic channel using fuzzy logic controller, image processing algorithm, and microvalves installed within microfluidic channel. A state space model has been derived from circuit analogy approach to describe the microfluidic network. Furthermore, a COMSOL-based study is primed for device structure by means of droplet generation and controlling the droplet through fitted valves. Moreover, an image processing algorithm based on active contours has been proposed in this research to track the movement of the droplet through the channel. This droplet controlling method is utterly based on fuzzy controller as well as camera images to move the droplet at desired position by controlling flow rates inside the fluidic channel using valves installed inside the microfluidic device. The results indicate that the fuzzy logic controller performs much better in terms of stability and faster response as compared to conventional proportional–integral–derivative controller.

[1]  Xize Niu,et al.  Electro-coalescence of digitally controlled droplets. , 2009, Analytical chemistry.

[2]  David Zhang,et al.  Reinitialization-Free Level Set Evolution via Reaction Diffusion , 2011, IEEE Transactions on Image Processing.

[3]  Xuefei Sun,et al.  Controlled dispensing and mixing of pico- to nanoliter volumes using on-demand droplet-based microfluidics , 2013, Microfluidics and nanofluidics.

[4]  Stephan Herminghaus,et al.  Controlled electrocoalescence in microfluidics: Targeting a single lamella , 2006 .

[5]  Chih-Yu Wang,et al.  Synthesis of uniform poly(d,l‐lactide) and poly(d,l‐lactide‐co‐glycolide) microspheres using a microfluidic chip for comparison , 2014, Electrophoresis.

[6]  James S. Duncan,et al.  Game-Theoretic Integration for Image Segmentation , 1999, IEEE Trans. Pattern Anal. Mach. Intell..

[7]  Alexandru Mihai Grumezescu,et al.  One-step synthesis of platinum nanoparticles loaded in alginate bubbles , 2014, Nanoscale Research Letters.

[8]  S. Cho,et al.  Creating, transporting, cutting, and merging liquid droplets by electrowetting-based actuation for digital microfluidic circuits , 2003 .

[9]  Anthony J. Yezzi,et al.  A Fully Global Approach to Image Segmentation via Coupled Curve Evolution Equations , 2002, J. Vis. Commun. Image Represent..

[10]  Nicolas Bremond,et al.  Decompressing emulsion droplets favors coalescence. , 2008, Physical review letters.

[11]  K. Jensen,et al.  Cells on chips , 2006, Nature.

[12]  D. Weitz,et al.  Electric control of droplets in microfluidic devices. , 2006, Angewandte Chemie.

[13]  Jean-Pierre Delville,et al.  An optical toolbox for total control of droplet microfluidics. , 2007, Lab on a chip.

[14]  Chang-Yu Chen,et al.  Sperm quality assessment via separation and sedimentation in a microfluidic device. , 2013, The Analyst.

[15]  Sosaku Ichikawa,et al.  Industrial lab-on-a-chip: design, applications and scale-up for drug discovery and delivery. , 2013, Advanced drug delivery reviews.

[16]  G. Whitesides,et al.  Applications of microfluidics in chemical biology. , 2006, Current opinion in chemical biology.

[17]  A. deMello.  Control and detection of chemical reactions in microfluidic systems , 2006, Nature.

[18]  M. Heckele,et al.  Review on micro molding of thermoplastic polymers , 2004 .

[19]  Michele Zagnoni,et al.  On-chip electrocoalescence of microdroplets as a function of voltage, frequency and droplet size. , 2009, Lab on a chip.

[20]  Andrew D Griffiths,et al.  Microfluidic production of droplet pairs. , 2008, Langmuir : the ACS journal of surfaces and colloids.

[21]  P. Abgrall,et al.  Lab-on-chip technologies: making a microfluidic network and coupling it into a complete microsystem—a review , 2007 .

[22]  Alexandru Mihai Grumezescu,et al.  A Microfluidic Chip Using Phenol Formaldehyde Resin for Uniform-Sized Polycaprolactone and Chitosan Microparticle Generation , 2013, Molecules.

[23]  Chih-Hui Yang,et al.  A Facile Fabrication of Alginate Microbubbles Using a Gas Foaming Reaction , 2013, Molecules.

[24]  Avishay Bransky,et al.  Coalescence-assisted generation of single nanoliter droplets with predefined composition. , 2011, Lab on a chip.

[25]  Wilhelm T S Huck,et al.  Surface-induced droplet fusion in microfluidic devices. , 2007, Lab on a chip.

[26]  Oliver J. Dressler,et al.  Droplet-Based Microfluidics , 2014, Journal of biomolecular screening.

[27]  Yi-Yao Hsu,et al.  Microfluidic synthesis of tail‐shaped alginate microparticles using slow sedimentation , 2013, Electrophoresis.