Old Methods for New Ideas: Genetic Dissection of the Determinants of Gene Expression Levels

There is increasing interest in understanding the molecular basis of complex traits. Initially, the genetic dissection of quantitative traits involved measurements of gross phenotypes. Subsequently, specific physiological and developmental components of individual traits have been dissected. Most recently, the underlying mechanisms of inheritance have been studied through various approaches that are supported by modern technological and methodological advances, namely quantitative trait locus/loci (QTL) analysis (Mackay, 2001; Mauricio, 2001; Doerge, 2002) and mutant analysis (Rossant and Spence, 1998; Hughes et al., 2000) in genetics; genome sequencing (Jang et al., 1999; The Arabidopsis Genome Initiative, 2000; Mouse Genome Sequencing Consortium, 2002) and gene expression analysis (Duggan et al., 1999; Lipshutz et al., 1999) in genomics; and protein structure analysis (Service, 1999) and protein assay (Kodadek, 2001; MacBeath, 2002)

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