Influence of enzyme concentration and thickness of the enzyme layer on the calibration curve of the continuously measuring glucose optode.

Since the first construction of enzyme electrodes by Updike and Hicks (1967) and Clark and Sachs (1968) bioelectrodes have been used in physiology, for example for the determination of glucose in blood and serum. One of the main advantages is that measurements can be made continuously for longer times. In these applications an enzyme reaction is coupled to the electrode process. A glucose electrode consists of a membrane of immobilized glucose oxidase, E. C. 1.1.3.4.. The enzyme catalyzes the oxidation of glucose to gluconic acid by dissolved oxygen from blood or serum. The difference in Po2 generated by the enzyme layer is a measure for glucose concentrations. The Clark electrode uses the reaction product H 2O2.