Flow Cytometry: A Practical Approach

Introduction to the principles of flow cytometry An introduction to fluorescence technology Preparing suspensions of single cells Flow sorting Immunofluorescence of surface markers Measurement of cytoplasmic and nuclear antigens Analysis of DNA - general methods Analysis of DNA-measurement of cell kinetics by the bromodeoxyuridine/anti-bromodeoxyuridine method Analysis of cell proliferation using the bromodeoxyuridine-Hoechst ethidium bromide method Chromosome analysis and sorting Intracellular ionized calcium, magnesium, membrane potential and pH Flow cytometry of RNA content Measurement of dynamic cellular events Further applications to cell biology.