Prevalence of Virulence Genes and Cytolethal Distending Toxin Production in Campylobacter jejuni Isolates from Diarrheal Patients in Bangladesh

ABSTRACT From 300 stool samples, 58 Campylobacter strains were isolated by standard microbiological and biochemical methods. Of these, 40 strains were identified as Campylobacter jejuni and 5 as Campylobacter coli. The presence of flaA (100%), cadF (100%), racR (100%), dnaJ (100%), pldA (100%), ciaB (95%), virB11 (0%), ceuE (82.5%), cdtA (97.5%), cdtB (97.5%), cdtC (97.5%), and wlaN (7.5%) genes was detected in C. jejuni by PCR. All C. jejuni strains but one produced cytolethal distending toxin in a HeLa cell assay.

[1]  A. Lastovica,et al.  Clinical Significance of Campylobacter and Related Species Other Than Campylobacter jejuni and Campylobacter coli , 2008 .

[2]  A. van Belkum,et al.  Lack of Association between the Presence of the pVir Plasmid and Bloody Diarrhea in Campylobacter jejuni Enteritis , 2006, Journal of Clinical Microbiology.

[3]  W. Müller,et al.  PCR detection of virulence-associated genes in Campylobacter jejuni strains with differential ability to invade Caco-2 cells and to colonize the chick gut. , 2006, Veterinary microbiology.

[4]  K. Itoh,et al.  Prevalence of 11 pathogenic genes of Campylobacter jejuni by PCR in strains isolated from humans, poultry meat and broiler and bovine faeces. , 2003, Journal of medical microbiology.

[5]  D. Sack,et al.  Phenotypic and Genotypic Characterization of Serologically Atypical Strains of Shigella flexneri Type 4 Isolated in Dhaka, Bangladesh , 2002, Journal of Clinical Microbiology.

[6]  M. Konkel,et al.  The pathogenesis of Campylobacter jejuni-mediated enteritis. , 2001, Current issues in intestinal microbiology.

[7]  J. Galán,et al.  CdtA, CdtB, and CdtC Form a Tripartite Complex That Is Required for Cytolethal Distending Toxin Activity , 2001, Infection and Immunity.

[8]  T. E. Hickey,et al.  Campylobacter jejuni Cytolethal Distending Toxin Mediates Release of Interleukin-8 from Intestinal Epithelial Cells , 2000, Infection and Immunity.

[9]  P. Hitchen,et al.  Phase variation of a β‐1,3 galactosyltransferase involved in generation of the ganglioside GM1‐like lipo‐oligosaccharide of Campylobacter jejuni , 2000, Molecular microbiology.

[10]  R. Alm,et al.  Involvement of a Plasmid in Virulence of Campylobacter jejuni 81-176 , 2000, Infection and Immunity.

[11]  C. Buswell,et al.  Characterisation of cytolethal distending toxin (CDT) mutants of Campylobacter jejuni. , 2000, Journal of medical microbiology.

[12]  Robert V. Tauxe,et al.  Epidemiology of Campylobacter jejuni infections in the United States and other industrialized nations , 2000 .

[13]  A. Faruque,et al.  Case-Control Study of Enteropathogens Associated with Childhood Diarrhea in Dhaka, Bangladesh , 1999, Journal of Clinical Microbiology.

[14]  M. Konkel,et al.  Identification of the enteropathogens Campylobacter jejuni and Campylobacter coli based on the cadF virulence gene and its product. , 1999, Journal of clinical microbiology.

[15]  D. Linton,et al.  PCR detection, identification to species level, and fingerprinting of Campylobacter jejuni and Campylobacter coli direct from diarrheic samples , 1997, Journal of clinical microbiology.

[16]  V. Aragon,et al.  Effect of cytolethal distending toxin on F-actin assembly and cell division in Chinese hamster ovary cells , 1997, Infection and immunity.

[17]  M. Collins,et al.  Specific identification of the enteropathogens Campylobacter jejuni and Campylobacter coli by using a PCR test based on the ceuE gene encoding a putative virulence determinant , 1997, Journal of clinical microbiology.

[18]  Y. Takeda,et al.  Examination of diarrheagenicity of cytolethal distending toxin: suckling mouse response to the products of the cdtABC genes of Shigella dysenteriae , 1997, Infection and immunity.

[19]  A. Faruque,et al.  Controlled study of cytolethal distending toxin-producing Escherichia coli infections in Bangladeshi children , 1996, Journal of clinical microbiology.

[20]  S. Park,et al.  Molecular characterization of a Campylobacter jejuni lipoprotein with homology to periplasmic siderophore-binding proteins , 1995, Journal of bacteriology.

[21]  D. Mahalanabis,et al.  Infectious agents causing acute watery diarrhoea in infants and young children in Bangladesh and their public health implications. , 1994, Journal of tropical pediatrics.

[22]  E. Pesci,et al.  Escherichia Coli Cytolethal Distending Toxin Cloning, Sequencing, and Expression of The , 2022 .

[23]  F. Huq,et al.  Effect of Campylobacter jejuni extracts and culture supernatants on cell culture. , 1989, The Journal of tropical medicine and hygiene.

[24]  H. Lior,et al.  A new heat-labile cytolethal distending toxin (CLDT) produced by Campylobacter spp. , 1988, Microbial pathogenesis.

[25]  W. Gransden,et al.  Gastroenteritis and encephalopathy associated with a strain of Escherichia coli 055:K59:H4 that produced a cytolethal distending toxin. , 1987, The Pediatric infectious disease journal.

[26]  B. Stoll,et al.  Surveillance of patients attending a diarrhoeal disease hospital in Bangladesh , 1982, British medical journal.

[27]  M. Blaser,et al.  Campylobacter enteritis: clinical and epidemiologic features. , 1979, Annals of internal medicine.