Motility enhancement of bacteria actuated microstructures using selective bacteria adhesion.

Microrobots developed by the technological advances are useful for application in various fields. Nevertheless, they have limitations with respect to their actuator and motility. Our experiments aim to determine whether a bioactuator using the flagellated bacteria Serratia marcescens would enhance the motility of microrobots. In this study, we investigate that the flagellated bacteria Serratia marcescens could be utilized as actuators for SU-8 microstructures by bovine serum albumin-selective patterning. Firstly, we analyze the adherence of the bacteria to the SU-8 micro cube by selective patterning using 5% BSA. The results show that number of attached-bacteria in the uncoated side of the selectively- coated micro cube with BSA increased by 200% compared with that in all sides of the non treated micro cube. Secondly, the selectively BSA coated micro cube had 210% higher motility than the uncoated micro cube. The results revealed that the bacteria patterned to a specific site using 5% BSA significantly increase the motility of the bacteria actuated microstructure.

[1]  Won-Gun Koh,et al.  Micropatterning of proteins on the surface of three-dimensional poly(ethylene glycol) hydrogel microstructures. , 2008, Analytica chimica acta.

[2]  Sylvain Martel,et al.  Flagellated Magnetotactic Bacteria as Controlled MRI-trackable Propulsion and Steering Systems for Medical Nanorobots Operating in the Human Microvasculature , 2009, Int. J. Robotics Res..

[3]  Michael V. Pishko,et al.  Biomems Materials and Fabrication Technology: Control of Mammalian Cell and Bacteria Adhesion on Substrates Micropatterned with Poly(ethylene Glycol) Hydrogels , 2022 .

[4]  Hugh F. Durrant-Whyte,et al.  Mobile robot localization by tracking geometric beacons , 1991, IEEE Trans. Robotics Autom..

[5]  Metin Sitti,et al.  Miniature devices: Voyage of the microrobots , 2009, Nature.

[6]  M. J. Kim,et al.  Control of microfabricated structures powered by flagellated bacteria using phototaxis , 2007 .

[7]  Metin Sitti,et al.  Effect of quantity and configuration of attached bacteria on bacterial propulsion of microbeads , 2008 .

[8]  Ali Khademhosseini,et al.  Direct Patterning of Protein‐ and Cell‐Resistant Polymeric Monolayers and Microstructures , 2003 .

[9]  Sukho Park,et al.  Two-dimensional locomotion of a microrobot with a novel stationary electromagnetic actuation system , 2009 .

[10]  D. Hansford,et al.  Fabrication of polymer microstructures for MEMS: sacrificial layer micromolding and patterned substrate micromolding , 2007, Biomedical microdevices.

[11]  D. Weibel,et al.  Bacterial Swarming: A Model System for Studying Dynamic Self-assembly. , 2009, Soft matter.

[12]  J. Hugenholtz,et al.  Fluorescent Method for Monitoring Cheese Starter Permeabilization and Lysis , 2001, Applied and Environmental Microbiology.

[13]  Lixin Dong,et al.  Artificial bacterial flagella: Fabrication and magnetic control , 2009 .

[14]  H. Berg,et al.  Moving fluid with bacterial carpets. , 2004, Biophysical journal.

[15]  D. Taghirad.  Ieee Transactions on Robotics and Automation 1 Robust Torque Control of Harmonic Drive Systems , 1997 .

[16]  C. Laflamme,et al.  Assessment of bacterial endospore viability with fluorescent dyes , 2004, Journal of applied microbiology.

[17]  A. Wolff,et al.  Micro patterning of cell and protein non-adhesive plasma polymerized coatings for biochip applications. , 2004, Lab on a chip.

[18]  Yoshiyuki Sowa,et al.  Bacterial flagellar motor , 2004, Quarterly Reviews of Biophysics.

[19]  Katsumi Imada,et al.  Molecular motors of the bacterial flagella. , 2008, Current opinion in structural biology.

[20]  Yuehuei H. An,et al.  Handbook of Bacterial adhesion : principles, methods, and applications , 2000 .

[21]  C. Lewis,et al.  Use of bacteria in anti-cancer therapies. , 2006, BioEssays : news and reviews in molecular, cellular and developmental biology.

[22]  Min Jun Kim,et al.  A novel method of microfabrication and manipulation of bacterial teamsters in low Reynolds number fluidic environments , 2008 .

[23]  Jung-Joon Min,et al.  Quantitative bioluminescence imaging of tumor-targeting bacteria in living animals , 2008, Nature Protocols.

[24]  Y. S. Kim,et al.  Micropatterning of bacteria on two-dimensional lattice protein surface observed by atomic force microscopy. , 2008, Ultramicroscopy.

[25]  Metin Sitti,et al.  Modeling and Experimental Characterization of an Untethered Magnetic Micro-Robot , 2009, Int. J. Robotics Res..