The experiment was carried out in Longgang,Kaiyang county of Guizhou province.The major cuticular components from cured leaves from three plant positions of four flue-cured tobacco varieties grown in Guizhou were analyzed after extracted with methylene chloride.The result showed that the major cuticular components of cured leaf were nicotine,neophytadiene,trichome exudates and alkanes,the amount of which varied among various varieties.The order of total cuticular components,neophytadiene and trichome exudates for four varieties,from low to high,was Nanjiang 3 to K326 to Guiyan 201 to Yunyan 85.The average content of rentinol was roughly equal to the total amount of all other terpenes,the average content of cembratriene-diol was more than that of cembratriene-ol,the level of α-cembratriene-diol was more than twice as that of β-cembratriene-diol.The average levels of alkanes from middle position leaves of all varieties were the lowest,further research is needed to explore the causes.